Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis
| Autor(a) principal: | |
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| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30461 |
Resumo: | 2020-01-01 |
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Ker, Henrique GamaVital, Wendel CouraSoares, Rodrigo Dian de Oliveira AguiarRoatt, Bruno MendesMoreira, Nádia das DoresCarneiro, Cláudia MartinsMachado, Evandro Marques de MenezesCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisGiunchetti, Rodolfo CordeiroAraújo, Márcio Sobreira SilvaCoelho, Eduardo Antonio FerrazLemos, Denise da SilveiraReis, Alexandre Barbosa2018-12-10T12:04:19Z2018-12-10T12:04:19Z2013KER, Henrique Gama et al. Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis. Clin Vaccine Immunol., v. 20, n. 12, p. 1792-1798, 2013.1556-6811https://www.arca.fiocruz.br/handle/icict/3046110.1128/CVI.00575-13porAmerican Society for MicrobiologyLeishmaniose visceral caninacanine visceral leishmaniasisEvaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Universidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Pós-Graduação em Infectologia e Medicina Tropical. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Renè Rachou Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Laboratório de Biotecnologia Aplicada ao Estudo das Leishmanioses. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunoparasitologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Programa de Pós Graduação em Ciências Farmacêuticas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilDiagnosing canine visceral leishmaniasis (CVL) is a critical challenge since conventional immunoserological tests still present some deficiencies. The current study evaluated a prototype flow cytometry serology test, using antigens and fluorescent antibodies that had been stored for 1 year at 4°C, on a broad range of serum samples. Noninfected control dogs and Leishmania infantum-infected dogs were tested, and the prototype test showed excellent performance in differentiating these groups with high sensitivity, specificity, positive and negative predictive values, and accuracy (100% in all analyses). When the CVL group was evaluated according to the dogs' clinical status, the prototype test showed outstanding accuracy in all groups with positive serology (asymptomatic II, oligosymptomatic, and symptomatic). However, in dogs which had positive results by PCR-restriction fragment length polymorphism (RFLP) but negative results by conventional serology (asymptomatic I), serological reactivity was not observed. Additionally, sera from 40 dogs immunized with different vaccines (Leishmune, Leish-Tec, or LBSap) did not present serological reactivity in the prototype test. Eighty-eight dogs infected with other pathogens (Trypanosoma cruzi, Leishmania braziliensis, Ehrlichia canis, and Babesia canis) were used to determine cross-reactivity and specificity, and the prototype test performed well, particularly in dogs infected with B. canis and E. canis (100% and 93.3% specificities, respectively). In conclusion, our data reinforce the potential of the prototype test for use as a commercial kit and highlight its outstanding performance even after storage for 1 year at 4°C. Moreover, the prototype test efficiently provided accurate CVL serodiagnosis with an absence of false-positive results in vaccinated dogs and minor cross-reactivity against other canine pathogens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30461/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALEvaluation of a prototype flow cytometry test for.pdfEvaluation of a prototype flow cytometry test for.pdfapplication/pdf4309660https://www.arca.fiocruz.br/bitstream/icict/30461/2/Evaluation%20of%20a%20prototype%20flow%20cytometry%20test%20for.pdf8ce96d26a0200aa912b78e33852d6d61MD52TEXTEvaluation of a prototype flow cytometry test for.pdf.txtEvaluation of a prototype flow cytometry test for.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| title |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| spellingShingle |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis Ker, Henrique Gama Leishmaniose visceral canina canine visceral leishmaniasis |
| title_short |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| title_full |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| title_fullStr |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| title_full_unstemmed |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| title_sort |
Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis |
| author |
Ker, Henrique Gama |
| author_facet |
Ker, Henrique Gama Vital, Wendel Coura Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Carneiro, Cláudia Martins Machado, Evandro Marques de Menezes Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Araújo, Márcio Sobreira Silva Coelho, Eduardo Antonio Ferraz Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| author_role |
author |
| author2 |
Vital, Wendel Coura Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Carneiro, Cláudia Martins Machado, Evandro Marques de Menezes Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Araújo, Márcio Sobreira Silva Coelho, Eduardo Antonio Ferraz Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ker, Henrique Gama Vital, Wendel Coura Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Carneiro, Cláudia Martins Machado, Evandro Marques de Menezes Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Araújo, Márcio Sobreira Silva Coelho, Eduardo Antonio Ferraz Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose visceral canina |
| topic |
Leishmaniose visceral canina canine visceral leishmaniasis |
| dc.subject.en.pt_BR.fl_str_mv |
canine visceral leishmaniasis |
| description |
2020-01-01 |
| publishDate |
2013 |
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2013 |
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2018-12-10T12:04:19Z |
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2018-12-10T12:04:19Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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KER, Henrique Gama et al. Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis. Clin Vaccine Immunol., v. 20, n. 12, p. 1792-1798, 2013. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/30461 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1556-6811 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/CVI.00575-13 |
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KER, Henrique Gama et al. Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis. Clin Vaccine Immunol., v. 20, n. 12, p. 1792-1798, 2013. 1556-6811 10.1128/CVI.00575-13 |
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American Society for Microbiology |
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American Society for Microbiology |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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