In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23127 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. |
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Oliveira, Nathalia SilvaAndrade, Cecilia V.Grinsztejn, BeatrizFriedman, Ruth K.Cunha, Cynthia B.Veloso, Valdiléa G.Coutinho, Jose R.Chicarino-Oliveira, Janice M.Ferreira, Dennis de C.Pereira, Luiza O. R.Amaro-Filho, Sergio M.Moreira, Miguel A.Nuovo, Gerard J.Nicol, Alcina F.2017-11-13T14:43:39Z2017-11-13T14:43:39Z2016OLIVEIRA, Nathalia Silva etal. In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients. International Journal of Clinical and Experimental Pathology, v. 9, n. 2, p. 1520-1532, 2016.1936-2625https://www.arca.fiocruz.br/handle/icict/23127enge-Century PublishingHPV/HIV-1Neoplasia Intraepitelial AnalResposta ImuneGenótipoHPV/HIV-1Anal Intraepithelial NeoplasiaImmune ResponseGenotypePapillomaviridaeHIV-1In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ. Brasil.Universidade Estácio de Sá. Departamento de Endodontia. Programa de pós graduação em odontologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Instituto Nacional do Cancer. Divisão de Genética. Rio de Janeiro, RJ. Brasil.Comprehensive Cancer Center, Ohio State University, Columbus, Ohio, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Human papillomavirus (HPV) is the main etiologic agent of lower genital tract cancers. The natural history of HPV infection and the immune response to HIV/HPV co-infection, particularly in the anal mucosa, is poorly understood. The aim was evaluate the in situ immune response in anorectal biopsies from HIV-infected patients. A total of 114 biopsies were analyzed by Tissue Micro-Array: 15 were from HIV-negative individuals with normal squamous epithelium and 99 from HIV-positive individuals (21 normal squamous epithelium, 39 with anal intra-epithelial lesion grade 1 and 39 with anal intra-epithelial lesion grade 2/3). PCR and sequencing were used to identify HPV DNA. Staining for CD4, CD8, Foxp3+, T-bet and IL-10 were analyzed via immunohistochemistry. HIV-positive patients with AIN 2/3 showed a lower number of CD4+ cells (< 50 cells/mm3) compared to HIV negative subjects (P = 0.01). HIV infected individuals showed a higher expression of FoxP3+ and IL-10 that correlated with the severity of the lesion (P = 0.002). A positive coefficient correlation was found between FoxP3+ and IL-10 (r = 0.34; P = 0.027). HPV DNA was detected in 93.4% (101/107) of the samples and the most common types were HPV 16 (26.9%), followed by HPV 6 (15.7%), HPV 59 (13%) and HPV 18 (10.2%). Our results showed a strong association between the increased T-reg cells and IL-10 expression in HIV-positive patients with AIN 2/3. HPV 16 was the most prevalent type. Our study suggests that the immune regulatory in situ profile may favor HPV persistence in HIV-positive individuals. Further in situ studies should be done in order to elucidate the development of anal cancer in HPV/HIV co-infected individuals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83080https://www.arca.fiocruz.br/bitstream/icict/23127/1/license.txtc0ab280de860857c9554497e24a9070dMD51ORIGINALOliveira_Nathalia_S_etal_IOC_2017.pdfOliveira_Nathalia_S_etal_IOC_2017.pdfapplication/pdf1341261https://www.arca.fiocruz.br/bitstream/icict/23127/2/Oliveira_Nathalia_S_etal_IOC_2017.pdf188a212005045eeaaae4280385a30d59MD52TEXTOliveira_Nathalia_S_etal_IOC_2017.pdf.txtOliveira_Nathalia_S_etal_IOC_2017.pdf.txtExtracted texttext/plain41920https://www.arca.fiocruz.br/bitstream/icict/23127/3/Oliveira_Nathalia_S_etal_IOC_2017.pdf.txtbb0af9441f23a6eb7738697527ddb9ffMD53icict/231272023-03-29 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| dc.title.pt_BR.fl_str_mv |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| title |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| spellingShingle |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients Oliveira, Nathalia Silva HPV/HIV-1 Neoplasia Intraepitelial Anal Resposta Imune Genótipo HPV/HIV-1 Anal Intraepithelial Neoplasia Immune Response Genotype Papillomaviridae HIV-1 |
| title_short |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| title_full |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| title_fullStr |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| title_full_unstemmed |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| title_sort |
In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients |
| author |
Oliveira, Nathalia Silva |
| author_facet |
Oliveira, Nathalia Silva Andrade, Cecilia V. Grinsztejn, Beatriz Friedman, Ruth K. Cunha, Cynthia B. Veloso, Valdiléa G. Coutinho, Jose R. Chicarino-Oliveira, Janice M. Ferreira, Dennis de C. Pereira, Luiza O. R. Amaro-Filho, Sergio M. Moreira, Miguel A. Nuovo, Gerard J. Nicol, Alcina F. |
| author_role |
author |
| author2 |
Andrade, Cecilia V. Grinsztejn, Beatriz Friedman, Ruth K. Cunha, Cynthia B. Veloso, Valdiléa G. Coutinho, Jose R. Chicarino-Oliveira, Janice M. Ferreira, Dennis de C. Pereira, Luiza O. R. Amaro-Filho, Sergio M. Moreira, Miguel A. Nuovo, Gerard J. Nicol, Alcina F. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Nathalia Silva Andrade, Cecilia V. Grinsztejn, Beatriz Friedman, Ruth K. Cunha, Cynthia B. Veloso, Valdiléa G. Coutinho, Jose R. Chicarino-Oliveira, Janice M. Ferreira, Dennis de C. Pereira, Luiza O. R. Amaro-Filho, Sergio M. Moreira, Miguel A. Nuovo, Gerard J. Nicol, Alcina F. |
| dc.subject.other.pt_BR.fl_str_mv |
HPV/HIV-1 Neoplasia Intraepitelial Anal Resposta Imune Genótipo |
| topic |
HPV/HIV-1 Neoplasia Intraepitelial Anal Resposta Imune Genótipo HPV/HIV-1 Anal Intraepithelial Neoplasia Immune Response Genotype Papillomaviridae HIV-1 |
| dc.subject.en.pt_BR.fl_str_mv |
HPV/HIV-1 Anal Intraepithelial Neoplasia Immune Response Genotype |
| dc.subject.decs.pt_BR.fl_str_mv |
Papillomaviridae HIV-1 |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. |
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2016 |
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2016 |
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2017-11-13T14:43:39Z |
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2017-11-13T14:43:39Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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OLIVEIRA, Nathalia Silva etal. In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients. International Journal of Clinical and Experimental Pathology, v. 9, n. 2, p. 1520-1532, 2016. |
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https://www.arca.fiocruz.br/handle/icict/23127 |
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1936-2625 |
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OLIVEIRA, Nathalia Silva etal. In situ FoxP3+ and IL-10 over-expression is associated with high grade anal lesions in HIV infected patients. International Journal of Clinical and Experimental Pathology, v. 9, n. 2, p. 1520-1532, 2016. 1936-2625 |
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e-Century Publishing |
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e-Century Publishing |
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