The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed

Bibliographic Details
Main Author: Freitas, Rafael Maciel de
Publication Date: 2013
Other Authors: Sylvestre, Gabriel, Gandini, Mariana, Koella, Jacob C.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/12623
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
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spelling Freitas, Rafael Maciel deSylvestre, GabrielGandini, MarianaKoella, Jacob C.2016-01-26T15:04:13Z2016-01-26T15:04:13Z2013FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1262310.1371/journal.pone.0065252engPublic Library of ScienceThe Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Université de Neuchätel. Institut de Biologie. Switzerland.Background: Dengue virus (DENV) is transmitted by Aedes aegypti, a species that lives in close association with human dwellings. The behavior of DENV-infected mosquitoes needs further investigation, especially regarding the potential influence of DENV on mosquito biting motivation and avidity. Methodology/Principal findings: We orally challenged 4–5 day-old Ae. aegypti females with a low passage DENV serotype - 2 (DENV-2) to test whether the virus influences motivation to feed (the likelihood that a mosquito obtains a blood-meal and the size of its blood meal) and avidity (the likelihood to re-feed after an interrupted first blood-meal). To assay motivation, we offered mosquitoes an anesthetized mouse for 2, 3, 4 or 5 minutes 7 or 14 days after the initial blood meals and measured the time they started feeding. 60.5% of the unexposed mosquitoes fed on the mouse, but only 40.5% of the positive ones did. Exposed but negative mosquitoes behaved similarly to unexposed ones (55.0% feeding). Thus DENV-2 infection decreased the mosquitoes’ motivation to feed. To assay avidity, we offered the same mosquitoes a mouse two hours after the first round of feeding, and we measured the time at which they started probing. The exposed (positive or negative) mosquitoes were more likely to re-feed than the unexposed ones and, in particular, the size of the previous bloodmeal that kept mosquitoes from re-feeding was larger in the exposed than in the unexposed mosquitoes. Thus, DENV-2 infection increased mosquito avidity. Conclusions/Significance: DENV-2 significantly decreased the mosquitoes’ motivation to feed, but increased their avidity (even after taking account the amount of blood previously imbibed). As these are important components of transmission, we expect that the changes of the blood-feeding behaviour impact the vectorial capacity Ae. aegypti for dengue.DengueVirus Serotype-2Aedes aegyptiAedesDengueVírus da Dengueinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12623/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariana2_gandini_etal_IOC_2013.pdfmariana2_gandini_etal_IOC_2013.pdfapplication/pdf364173https://www.arca.fiocruz.br/bitstream/icict/12623/2/mariana2_gandini_etal_IOC_2013.pdfea2cb4379c6859d6547f6b4257669d30MD52TEXTmariana2_gandini_etal_IOC_2013.pdf.txtmariana2_gandini_etal_IOC_2013.pdf.txtExtracted texttext/plain30285https://www.arca.fiocruz.br/bitstream/icict/12623/3/mariana2_gandini_etal_IOC_2013.pdf.txtb4e002f0e6bb11273fa63d70f97cd196MD53icict/126232023-09-05 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dc.title.pt_BR.fl_str_mv The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
title The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
spellingShingle The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
Freitas, Rafael Maciel de
Dengue
Virus Serotype-2
Aedes aegypti
Aedes
Dengue
Vírus da Dengue
title_short The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
title_full The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
title_fullStr The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
title_full_unstemmed The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
title_sort The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
author Freitas, Rafael Maciel de
author_facet Freitas, Rafael Maciel de
Sylvestre, Gabriel
Gandini, Mariana
Koella, Jacob C.
author_role author
author2 Sylvestre, Gabriel
Gandini, Mariana
Koella, Jacob C.
author2_role author
author
author
dc.contributor.author.fl_str_mv Freitas, Rafael Maciel de
Sylvestre, Gabriel
Gandini, Mariana
Koella, Jacob C.
dc.subject.en.pt_BR.fl_str_mv Dengue
Virus Serotype-2
Aedes aegypti
topic Dengue
Virus Serotype-2
Aedes aegypti
Aedes
Dengue
Vírus da Dengue
dc.subject.decs.pt_BR.fl_str_mv Aedes
Dengue
Vírus da Dengue
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-01-26T15:04:13Z
dc.date.available.fl_str_mv 2016-01-26T15:04:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12623
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0065252
identifier_str_mv FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013.
1932-6203
10.1371/journal.pone.0065252
url https://www.arca.fiocruz.br/handle/icict/12623
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12623/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12623/2/mariana2_gandini_etal_IOC_2013.pdf
https://www.arca.fiocruz.br/bitstream/icict/12623/3/mariana2_gandini_etal_IOC_2013.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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