Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats

Detalhes bibliográficos
Autor(a) principal: Barbosa Junior, Aryon de Almeida
Data de Publicação: 1992
Outros Autores: Zhou, Hui, Hiiltenschmidt, Dieter, Totovic, Vladimir, Jurilj, Nenad, Pfeifer, Ulrich
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26820
Resumo: Deutsche Forschungsgemeinschaft (Pf 95/2-1).
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spelling Barbosa Junior, Aryon de AlmeidaZhou, HuiHiiltenschmidt, DieterTotovic, VladimirJurilj, NenadPfeifer, Ulrich2018-06-12T12:42:07Z2018-06-12T12:42:07Z1992BARBOSA JUNIOR, A. A. et al. Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats. Virchows Archiv B Cell Pathology, v. 61, p. 359-366, 1992.0340-6075https://www.arca.fiocruz.br/handle/icict/26820Deutsche Forschungsgemeinschaft (Pf 95/2-1).Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Bonn. Department of Pathology. Federal Republic of Germany, GermanyUniversity of Bonn. Department of Pathology. Federal Republic of Germany, GermanyUniversity of Bonn. Department of Pathology. Federal Republic of Germany, GermanyUniversity of Zagreb. Stomatological Faculty. Department of Biology. YugoslaviaUniversity of Bonn. Department of Pathology. Federal Republic of Germany, GermanyTo examine the significance of anti-catabolism in renal hypertrophy, cellular autophagy was investigated by electron microscopic morphometry in proximal tubular cells (PTCs) of the outer cortex of the rat kidney after the induction of diabetes mellitus by streptozotocin (STZ) and after unilateral nephrectomy. Adult male Sprague-Dawley rats were divided into three groups and killed by retrograde perfusion fixation, 1, 2 and 3 days after the induction of diabetes (group D; n = 24), after unilateral nephrectomy (group N; n = 24) and after combined treatment (group DN; n=24). Untreated, agematched litter mates served as controls (group C; n = 24). By comparison with these controls, the left kidney to initial body weight ratio was increased by 8, 23, and 15% in group D animals, by 8, 23, and 24% in group N animals, and by 10, 21, and 25% in group DN animals at the first, second and third day, respectively. Quantitative evaluation of large test areas showed that the volume and numerical densities of autophagic vacuoles (AVs) in PTCs were significantly lower in these hypertrophed kidneys than in the controls. The average reduction in AV volume density was about 65% in group D animals, about 50% in group N animals and about 75% in group DN animals. These data show that autophagic degradation of cytoplasmic components in PTCs is inhibited in renal hypertrophy independently of the growth stimulus, i.e. uninephrectomy or diabetes. Since insulin per se inhibits cellular autophagy in PTCs, the expected effect of insulin dificiency seems to be counteracted by as yet undefined stimuli that may be related to metabolic work loadengSpringer VerlagAutofagia CelularMorfometria UltraestruturalTúbulos ProximalHipertrofia AdaptativaUninephrectomy-Diabetes ExperimentalCellular autophagyUltrastructural morphometryProximal tubulesAdaptative hypertrophyUninephrectomy-Experimental diabetesInhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26820/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBarbosa JA Inhibition of cellular autophagy in 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dc.title.pt_BR.fl_str_mv Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
title Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
spellingShingle Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
Barbosa Junior, Aryon de Almeida
Autofagia Celular
Morfometria Ultraestrutural
Túbulos Proximal
Hipertrofia Adaptativa
Uninephrectomy-Diabetes Experimental
Cellular autophagy
Ultrastructural morphometry
Proximal tubules
Adaptative hypertrophy
Uninephrectomy-Experimental diabetes
title_short Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
title_full Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
title_fullStr Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
title_full_unstemmed Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
title_sort Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats
author Barbosa Junior, Aryon de Almeida
author_facet Barbosa Junior, Aryon de Almeida
Zhou, Hui
Hiiltenschmidt, Dieter
Totovic, Vladimir
Jurilj, Nenad
Pfeifer, Ulrich
author_role author
author2 Zhou, Hui
Hiiltenschmidt, Dieter
Totovic, Vladimir
Jurilj, Nenad
Pfeifer, Ulrich
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa Junior, Aryon de Almeida
Zhou, Hui
Hiiltenschmidt, Dieter
Totovic, Vladimir
Jurilj, Nenad
Pfeifer, Ulrich
dc.subject.other.pt_BR.fl_str_mv Autofagia Celular
Morfometria Ultraestrutural
Túbulos Proximal
Hipertrofia Adaptativa
Uninephrectomy-Diabetes Experimental
topic Autofagia Celular
Morfometria Ultraestrutural
Túbulos Proximal
Hipertrofia Adaptativa
Uninephrectomy-Diabetes Experimental
Cellular autophagy
Ultrastructural morphometry
Proximal tubules
Adaptative hypertrophy
Uninephrectomy-Experimental diabetes
dc.subject.en.pt_BR.fl_str_mv Cellular autophagy
Ultrastructural morphometry
Proximal tubules
Adaptative hypertrophy
Uninephrectomy-Experimental diabetes
description Deutsche Forschungsgemeinschaft (Pf 95/2-1).
publishDate 1992
dc.date.issued.fl_str_mv 1992
dc.date.accessioned.fl_str_mv 2018-06-12T12:42:07Z
dc.date.available.fl_str_mv 2018-06-12T12:42:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA JUNIOR, A. A. et al. Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats. Virchows Archiv B Cell Pathology, v. 61, p. 359-366, 1992.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26820
dc.identifier.issn.pt_BR.fl_str_mv 0340-6075
identifier_str_mv BARBOSA JUNIOR, A. A. et al. Inhibition of cellular autophagy in proximal tubular cells of the kidney in streptozotocin-diabetic and uninephrectomized rats. Virchows Archiv B Cell Pathology, v. 61, p. 359-366, 1992.
0340-6075
url https://www.arca.fiocruz.br/handle/icict/26820
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26820/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26820/2/Barbosa%20JA%20Inhibition%20of%20cellular%20autophagy%20in%20proximal.....pdf
https://www.arca.fiocruz.br/bitstream/icict/26820/3/Barbosa%20JA%20Inhibition%20of%20cellular%20autophagy%20in%20proximal.....pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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