Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.

Detalhes bibliográficos
Autor(a) principal: Souza, Aracele Maria
Data de Publicação: 2015
Outros Autores: Araújo, Flavia Carolina Faustino de, Fontes, Cor de Jesus Fernandes., Carvalho, Luzia Helena de, Brito, Cristiana Ferreira Alves de, Sousa, Taís Nóbrega de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12904
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
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spelling Souza, Aracele MariaAraújo, Flavia Carolina Faustino deFontes, Cor de Jesus Fernandes.Carvalho, Luzia Helena deBrito, Cristiana Ferreira Alves deSousa, Taís Nóbrega de2016-02-29T17:50:22Z2016-02-29T17:50:22Z2015SOUZA, Aracele Maria de et al. Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology. Malar J. , vol. 14, p. 330, 2015.1475-2875https://www.arca.fiocruz.br/handle/icict/1290410.1186/s12936-015-0846-5engBioMed CentralMultiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Mato Grosso. Hospital Julio Muller. Cuiabá, MT, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilBACKGROUND: Plasmodium vivax infections commonly contain multiple genetically distinct parasite clones. The detection of multiple-clone infections depends on several factors, such as the accuracy of the genotyping method, and the type and number of the molecular markers analysed. Characterizing the multiplicity of infection has broad implications that range from population genetic studies of the parasite to malaria treatment and control. This study compared and evaluated the efficiency of neutral and non-neutral markers that are widely used in studies of molecular epidemiology to detect the multiplicity of P. vivax infection. METHODS: The performance of six markers was evaluated using 11 mixtures of DNA with well-defined proportions of two different parasite genotypes for each marker. These mixtures were generated by mixing cloned PCR products or patient-derived genomic DNA. In addition, 51 samples of natural infections from the Brazil were genotyped for all markers. The PCR-capillary electrophoresis-based method was used to permit direct comparisons among the markers. The criteria for differentiating minor peaks from artifacts were also evaluated. RESULTS: The analysis of DNA mixtures showed that the tandem repeat MN21 and the polymorphic blocks 2 (msp1B2) and 10 (msp1B10) of merozoite surface protein-1 allowed for the estimation of the expected ratio of both alleles in the majority of preparations. Nevertheless, msp1B2 was not able to detect the majority of multiple-clone infections in field samples; it identified only 6 % of these infections. The merozoite surface protein-3 alpha and microsatellites (PvMS6 and PvMS7) did not accurately estimate the relative clonal proportions in artificial mixtures, but the microsatellites performed well in detecting natural multiple-clone infections. Notably, the use of a less stringent criterion to score rare alleles significantly increased the sensitivity of the detection of multi-clonal infections. CONCLUSIONS: Depending on the type of marker used, a considerable amplification bias was observed, which may have serious implications for the characterization of the complexity of a P. vivax infection. Based on the performance of markers in artificial mixtures of DNA and natural infections, a minimum panel of four genetic markers (PvMS6, PvMS7, MN21, and msp1B10) was defined, and these markers are highly informative regarding the genetic variability of P. vivax populations.Plasmodium vivaxP. vivax infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12904/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMultiple-clone infections of Plasmodium.pdfMultiple-clone infections of Plasmodium.pdfapplication/pdf5467763https://www.arca.fiocruz.br/bitstream/icict/12904/2/Multiple-clone%20infections%20of%20Plasmodium.pdfb4719a5dd04db8f670d04a87ecc9303fMD52TEXTMultiple-clone infections of Plasmodium.pdf.txtMultiple-clone infections of Plasmodium.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
title Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
spellingShingle Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
Souza, Aracele Maria
Plasmodium vivax
P. vivax infection
title_short Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
title_full Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
title_fullStr Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
title_full_unstemmed Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
title_sort Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology.
author Souza, Aracele Maria
author_facet Souza, Aracele Maria
Araújo, Flavia Carolina Faustino de
Fontes, Cor de Jesus Fernandes.
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
author_role author
author2 Araújo, Flavia Carolina Faustino de
Fontes, Cor de Jesus Fernandes.
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Aracele Maria
Araújo, Flavia Carolina Faustino de
Fontes, Cor de Jesus Fernandes.
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
P. vivax infection
topic Plasmodium vivax
P. vivax infection
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-02-29T17:50:22Z
dc.date.available.fl_str_mv 2016-02-29T17:50:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Aracele Maria de et al. Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology. Malar J. , vol. 14, p. 330, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12904
dc.identifier.issn.none.fl_str_mv 1475-2875
dc.identifier.doi.none.fl_str_mv 10.1186/s12936-015-0846-5
identifier_str_mv SOUZA, Aracele Maria de et al. Multiple-clone infections of Plasmodium vivax: definition of a panel of markers for molecular epidemiology. Malar J. , vol. 14, p. 330, 2015.
1475-2875
10.1186/s12936-015-0846-5
url https://www.arca.fiocruz.br/handle/icict/12904
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12904/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/12904/2/Multiple-clone%20infections%20of%20Plasmodium.pdf
https://www.arca.fiocruz.br/bitstream/icict/12904/3/Multiple-clone%20infections%20of%20Plasmodium.pdf.txt
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