Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy

Bibliographic Details
Main Author: Fernandes, Jill N.
Publication Date: 2018
Other Authors: Santos, Lilha M. B. dos, Carneiro, Thaís Chouin, Pavan, Márcio G., Garcia, Gabriela A., David, Mariana R., Beier, John C., Dowell, Floyd E., Freitas, Rafael Maciel de, Sikulu-Lord, Maggy T.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/27775
Summary: University of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.
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spelling Fernandes, Jill N.Santos, Lilha M. B. dosCarneiro, Thaís ChouinPavan, Márcio G.Garcia, Gabriela A.David, Mariana R.Beier, John C.Dowell, Floyd E.Freitas, Rafael Maciel deSikulu-Lord, Maggy T.2018-08-02T11:18:06Z2018-08-02T11:18:06Z2018FERNANDES, Jill N. et al. Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Sci. Adv., v.4, eaat0496, 6p, May 2018.2375-2548https://www.arca.fiocruz.br/handle/icict/27775engAmerican Association for the Advancement of ScienceZika VirusAedes AegyptiEspectroscopia de Luz Próxima ao Infravermelhodetecção não invasivaZika VirusAedes aegyptinoninvasive detectionnear-infrared spectroscopyEspectroscopia de Luz Próxima ao InfravermelhoRapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.University of Miami Miller School of Medicine. Department of Public Health Services. Miami, FL, USA.U.S. Department of Agriculture. Agricultural Research Service. Center for Grain and Animal Health Research. Manhatta, USA / University of Miami Miller School of Medicine. Department of Publica Health. Miami, FL, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.University of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, amajor challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection inmosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27775/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLilha_santos_etal_IOC_2018.pdfLilha_santos_etal_IOC_2018.pdfapplication/pdf554151https://www.arca.fiocruz.br/bitstream/icict/27775/2/Lilha_santos_etal_IOC_2018.pdf91c0c589349f5e2126364e72724881dfMD52TEXTLilha_santos_etal_IOC_2018.pdf.txtLilha_santos_etal_IOC_2018.pdf.txtExtracted texttext/plain40179https://www.arca.fiocruz.br/bitstream/icict/27775/3/Lilha_santos_etal_IOC_2018.pdf.txt232672b5afbd698517b2044c23978867MD53icict/277752018-08-15 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dc.title.pt_BR.fl_str_mv Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
title Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
spellingShingle Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
Fernandes, Jill N.
Zika Virus
Aedes Aegypti
Espectroscopia de Luz Próxima ao Infravermelho
detecção não invasiva
Zika Virus
Aedes aegypti
noninvasive detection
near-infrared spectroscopy
Espectroscopia de Luz Próxima ao Infravermelho
title_short Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
title_full Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
title_fullStr Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
title_full_unstemmed Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
title_sort Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy
author Fernandes, Jill N.
author_facet Fernandes, Jill N.
Santos, Lilha M. B. dos
Carneiro, Thaís Chouin
Pavan, Márcio G.
Garcia, Gabriela A.
David, Mariana R.
Beier, John C.
Dowell, Floyd E.
Freitas, Rafael Maciel de
Sikulu-Lord, Maggy T.
author_role author
author2 Santos, Lilha M. B. dos
Carneiro, Thaís Chouin
Pavan, Márcio G.
Garcia, Gabriela A.
David, Mariana R.
Beier, John C.
Dowell, Floyd E.
Freitas, Rafael Maciel de
Sikulu-Lord, Maggy T.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Jill N.
Santos, Lilha M. B. dos
Carneiro, Thaís Chouin
Pavan, Márcio G.
Garcia, Gabriela A.
David, Mariana R.
Beier, John C.
Dowell, Floyd E.
Freitas, Rafael Maciel de
Sikulu-Lord, Maggy T.
dc.subject.other.pt_BR.fl_str_mv Zika Virus
Aedes Aegypti
Espectroscopia de Luz Próxima ao Infravermelho
detecção não invasiva
topic Zika Virus
Aedes Aegypti
Espectroscopia de Luz Próxima ao Infravermelho
detecção não invasiva
Zika Virus
Aedes aegypti
noninvasive detection
near-infrared spectroscopy
Espectroscopia de Luz Próxima ao Infravermelho
dc.subject.en.pt_BR.fl_str_mv Zika Virus
Aedes aegypti
noninvasive detection
near-infrared spectroscopy
dc.subject.decs.pt_BR.fl_str_mv Espectroscopia de Luz Próxima ao Infravermelho
description University of Queensland. Queensland Alliance for Agriculture and Food Innovation. St. Lucia, Queensland, Australia.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-08-02T11:18:06Z
dc.date.available.fl_str_mv 2018-08-02T11:18:06Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, Jill N. et al. Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Sci. Adv., v.4, eaat0496, 6p, May 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27775
dc.identifier.issn.pt_BR.fl_str_mv 2375-2548
identifier_str_mv FERNANDES, Jill N. et al. Rapid, noninvasive detection of Zika virus in Aedes aegypti mosquitoes by near-infrared spectroscopy. Sci. Adv., v.4, eaat0496, 6p, May 2018.
2375-2548
url https://www.arca.fiocruz.br/handle/icict/27775
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Association for the Advancement of Science
publisher.none.fl_str_mv American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27775/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/27775/2/Lilha_santos_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/27775/3/Lilha_santos_etal_IOC_2018.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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