Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type

Detalhes bibliográficos
Autor(a) principal: Martins, Gustavo Ferreira
Data de Publicação: 2011
Outros Autores: Serrão, José Eduardo, Ortigão, José Marcelo Ramalho, Pimenta, Paulo Filemon Paolucci
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8937
Resumo: Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, Brazil
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spelling Martins, Gustavo FerreiraSerrão, José EduardoOrtigão, José Marcelo RamalhoPimenta, Paulo Filemon Paolucci2014-11-21T14:54:12Z2014-11-21T14:54:12Z2011MARTINS, Gustavo Ferreira et al. Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type. Microsc Res Techniq, v. 74 n.11 p. 1032-1039, 2011.1059-910Xhttps://www.arca.fiocruz.br/handle/icict/893710.1002/jemt.20990.engWiley-LissHistochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet typeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, BrazilUniversidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, BrazilKansas State University. Department of Entomology. Manhattan, KS, USAFundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilAedes aegypti is the principal vector of dengue world wide and a major vector of urban yellow fever. Despite its epidemiological importance, not much is known regarding cellular and structural changes in the fat body in this mosquito. Here, we applied light and transmission electron microscopies in order to investigate structural changes in the fat body of three groups of A. aegypti females: newly emerged, 18-day-old sugar-fed, and 18-day-old blood-fed. The fat body consists of a layer of cells attached to the abdomen integument, formed by trophocytes and oenocytes. Trophocytes are strongly positive for carbohydrates, while oenocytes are strongly positive for proteins and lipids. Ultrastructural analyses of trophocytes from newly emerged and 18-day-old blood-fed indicate that these cells are rich in glycogen and free ribosomes. Many lipid droplets and protein granules, which are broken down after the blood meal, are also detected. In 18-day-old sugar-fed, trophocytes display a disorganized cytoplasm filled with lipid droplets, and reduced numbers of free ribosomes, glycogen, rough endoplasmic reticulum (RER) and mitochondria. Following a blood meal, the RER and mitochondria display enlarged sizes, suggestive of increased activity. In regards to oenocytes, these cells display an electron-dense cytoplasm and plasma membrane infoldings facing the hemolymph. As the A. aegypti female ages, trophocyte and oenocyte cell nuclei become larger but decrease in diameter after blood feeding. Our findings suggest that the trophocytes and oenocytes remodeling is likely involved in functional changes of fat body that take place during aging and following a blood meal in A. aegypti females.Aedes aegyptitrophocytesoenocytesmorphologyfat bodymosquitoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8937/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALHistochemical and ultrastructural studies of the mosquito Aedes aegypti fat body.pdfHistochemical and ultrastructural studies of the mosquito Aedes aegypti fat body.pdfapplication/pdf1072218https://www.arca.fiocruz.br/bitstream/icict/8937/2/Histochemical%20and%20ultrastructural%20studies%20of%20the%20mosquito%20Aedes%20aegypti%20fat%20body.pdf41d19d083611a6e34c32fa468e2fcd7fMD52TEXTHistochemical and ultrastructural studies of the mosquito Aedes aegypti fat body.pdf.txtHistochemical and ultrastructural studies of the mosquito Aedes aegypti fat body.pdf.txtExtracted texttext/plain36786https://www.arca.fiocruz.br/bitstream/icict/8937/3/Histochemical%20and%20ultrastructural%20studies%20of%20the%20mosquito%20Aedes%20aegypti%20fat%20body.pdf.txt8c62b6323e871dc7bf5e72041eeb64beMD53icict/89372019-06-19 10:11:50.22oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:11:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
title Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
spellingShingle Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
Martins, Gustavo Ferreira
Aedes aegypti
trophocytes
oenocytes
morphology
fat body
mosquito
title_short Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
title_full Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
title_fullStr Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
title_full_unstemmed Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
title_sort Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type
author Martins, Gustavo Ferreira
author_facet Martins, Gustavo Ferreira
Serrão, José Eduardo
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
author_role author
author2 Serrão, José Eduardo
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
author2_role author
author
author
dc.contributor.author.fl_str_mv Martins, Gustavo Ferreira
Serrão, José Eduardo
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
trophocytes
oenocytes
morphology
fat body
mosquito
topic Aedes aegypti
trophocytes
oenocytes
morphology
fat body
mosquito
description Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-21T14:54:12Z
dc.date.available.fl_str_mv 2014-11-21T14:54:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Gustavo Ferreira et al. Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type. Microsc Res Techniq, v. 74 n.11 p. 1032-1039, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8937
dc.identifier.issn.none.fl_str_mv 1059-910X
dc.identifier.doi.none.fl_str_mv 10.1002/jemt.20990.
identifier_str_mv MARTINS, Gustavo Ferreira et al. Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: Effects of aging and diet type. Microsc Res Techniq, v. 74 n.11 p. 1032-1039, 2011.
1059-910X
10.1002/jemt.20990.
url https://www.arca.fiocruz.br/handle/icict/8937
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-Liss
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collection Repositório Institucional da FIOCRUZ (ARCA)
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