Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon

Detalhes bibliográficos
Autor(a) principal: Chaves, Lana Bitencourt
Data de Publicação: 2017
Outros Autores: Silva, Daiana de Souza Perce da, Silva, Rodrigo Nunes Rodrigues da, Silva, João Hermínio Martins da, Cassiano, Gustavo Capatti, Machado, Ricardo Luiz Dantas, Riccio, Lilian Rose Pratt, Banic, Dalma Maria, Lima Junior, Josué da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23276
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Chaves, Lana BitencourtSilva, Daiana de Souza Perce daSilva, Rodrigo Nunes Rodrigues daSilva, João Hermínio Martins daCassiano, Gustavo CapattiMachado, Ricardo Luiz DantasRiccio, Lilian Rose PrattBanic, Dalma MariaLima Junior, Josué da Costa2017-11-21T10:18:00Z2017-11-21T10:18:00Z2017CHAVES, Lana Bitencourt et al. Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 11, n. 2, p. 1-19, Feb. 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2327610.1371/journal.pntd.00053441935-2735engPublic Library of SciencePlasmodium vivaxEsporozoítosAmazônia BrasileiraEpitopos de Linfócito BOokinetesMaláriaPlasmodium vivaxSporozoitesBrazilian Amazonpotential B-cell epitopesookinetesMalariaPlasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Ceará. Grupo de Modelagem Computacional. Fortaleza, CE, Brasil.Universidade Estadual de Campinas. Departamento de Genética, Evolução e Bioagentes. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil.Instituto Evandro Chagas. Laboratório de Imunogenética da Malária. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.The Plasmodium vivax Cell-traversal protein for ookinetes and sporozoites (PvCelTOS) plays an important role in the traversal of host cells. Although essential to PvCelTOS progress as a vaccine candidate, its genetic diversity remains uncharted. Therefore, we investigated the PvCelTOS genetic polymorphism in 119 field isolates from five different regions of Brazilian Amazon (Manaus, Novo Repartimento, Porto Velho, Plácido de Castro and Oiapoque). Moreover, we also evaluated the potential impact of non-synonymous mutations found in the predicted structure and epitopes of PvCelTOS. The field isolates showed high similarity (99.3% of bp) with the reference Sal-1 strain, presenting only four Single-Nucleotide Polymorphisms (SNP) at positions 24A, 28A, 109A and 352C. The frequency of synonymous C109A (82%) was higher than all others (p<0.0001). However, the non-synonymous G28A and G352C were observed in 9.2% and 11.7% isolates. The great majority of the isolates (79.8%) revealed complete amino acid sequence homology with Sal-1, 10.9% presented complete homology with Brazil I and two undescribed PvCelTOS sequences were observed in 9.2% field isolates. Concerning the prediction analysis, the N-terminal substitution (Gly10Ser) was predicted to be within a B-cell epitope (PvCelTOS Accession Nos. AB194053.1) and exposed at the protein surface, while the Val118Leu substitution was not a predicted epitope. Therefore, our data suggest that although G28A SNP might interfere in potential B-cell epitopes at PvCelTOS N-terminal region the gene sequence is highly conserved among the isolates from different geographic regions, which is an important feature to be taken into account when evaluating its potential as a vaccine candidate.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23276/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjosue_limajr_etal_IOC_2017.pdfjosue_limajr_etal_IOC_2017.pdfapplication/pdf1617736https://www.arca.fiocruz.br/bitstream/icict/23276/2/josue_limajr_etal_IOC_2017.pdfd19303558cd2922f63a2881e45025860MD52TEXTjosue_limajr_etal_IOC_2017.pdf.txtjosue_limajr_etal_IOC_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
title Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
spellingShingle Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
Chaves, Lana Bitencourt
Plasmodium vivax
Esporozoítos
Amazônia Brasileira
Epitopos de Linfócito B
Ookinetes
Malária
Plasmodium vivax
Sporozoites
Brazilian Amazon
potential B-cell epitopes
ookinetes
Malaria
title_short Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
title_full Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
title_fullStr Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
title_full_unstemmed Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
title_sort Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon
author Chaves, Lana Bitencourt
author_facet Chaves, Lana Bitencourt
Silva, Daiana de Souza Perce da
Silva, Rodrigo Nunes Rodrigues da
Silva, João Hermínio Martins da
Cassiano, Gustavo Capatti
Machado, Ricardo Luiz Dantas
Riccio, Lilian Rose Pratt
Banic, Dalma Maria
Lima Junior, Josué da Costa
author_role author
author2 Silva, Daiana de Souza Perce da
Silva, Rodrigo Nunes Rodrigues da
Silva, João Hermínio Martins da
Cassiano, Gustavo Capatti
Machado, Ricardo Luiz Dantas
Riccio, Lilian Rose Pratt
Banic, Dalma Maria
Lima Junior, Josué da Costa
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chaves, Lana Bitencourt
Silva, Daiana de Souza Perce da
Silva, Rodrigo Nunes Rodrigues da
Silva, João Hermínio Martins da
Cassiano, Gustavo Capatti
Machado, Ricardo Luiz Dantas
Riccio, Lilian Rose Pratt
Banic, Dalma Maria
Lima Junior, Josué da Costa
dc.subject.other.pt_BR.fl_str_mv Plasmodium vivax
Esporozoítos
Amazônia Brasileira
Epitopos de Linfócito B
Ookinetes
Malária
topic Plasmodium vivax
Esporozoítos
Amazônia Brasileira
Epitopos de Linfócito B
Ookinetes
Malária
Plasmodium vivax
Sporozoites
Brazilian Amazon
potential B-cell epitopes
ookinetes
Malaria
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Sporozoites
Brazilian Amazon
potential B-cell epitopes
ookinetes
Malaria
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-21T10:18:00Z
dc.date.available.fl_str_mv 2017-11-21T10:18:00Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAVES, Lana Bitencourt et al. Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 11, n. 2, p. 1-19, Feb. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23276
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0005344
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv CHAVES, Lana Bitencourt et al. Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (PvCelTOS) gene sequence and potential epitopes are highly conserved among isolates from different regions of Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 11, n. 2, p. 1-19, Feb. 2017.
1935-2727
10.1371/journal.pntd.0005344
1935-2735
url https://www.arca.fiocruz.br/handle/icict/23276
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/23276/2/josue_limajr_etal_IOC_2017.pdf
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