RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions

Detalhes bibliográficos
Autor(a) principal: Silva, Amanda Perse da
Data de Publicação: 2014
Outros Autores: Lopes, Juliana Freitas, Paula, Vanessa Salete de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10268
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Amanda Perse daLopes, Juliana FreitasPaula, Vanessa Salete de2015-05-08T14:08:46Z2015-05-08T14:08:46Z2014SILVA, Amanda Perse da; LOPES, Juliana Freitas; PAULA, Vanessa Salete de. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions. The Brazilian Journal of Infectious Diseases, v.18, n.4, p.441-444, 2014.1413-8670https://www.arca.fiocruz.br/handle/icict/1026810.1016/j.bjid.2014.01.011engElsevierHerpes simplesHerpes simplex virus type 1Real-time PCRsiRNARNA Interferente PequenoHerpes SimplesRNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.The aim of this study was to evaluate the use of RNA interference to inhibit herpes simplexvirus type-1 replication in vitro. For herpes simplex virus type-1 gene silencing, three dif-ferent small interfering RNAs (siRNAs) targeting the herpes simplex virus type-1 UL39 gene(sequence si-UL 39-1, si-UL 39-2, and si-UL 39-3) were used, which encode the large sub-unit of ribonucleotide reductase, an essential enzyme for DNA synthesis. Herpes simplexvirus type-1 was isolated from saliva samples and mucocutaneous lesions from infectedpatients. All mucocutaneous lesions’ samples were positive for herpes simplex virus type-1by real-time PCR and by virus isolation; all herpes simplex virus type-1 from saliva sampleswere positive by real-time PCR and 50% were positive by virus isolation. The levels of her-pes simplex virus type-1 DNA remaining after siRNA treatment were assessed by real-timePCR, whose results demonstrated that the effect of siRNAs on gene expression depends onsiRNA concentration. The three siRNA sequences used were able to inhibit viral replication,assessed by real-time PCR and plaque assays and among them, the sequence si-UL 39-1was the most effective. This sequence inhibited 99% of herpes simplex virus type-1 replica-tion. The results demonstrate that silencing herpes simplex virus type-1 UL39 expressionby siRNAs effectively inhibits herpes simplex virus type-1 replication, suggesting that siRNAbased antiviral strategy may be a potential therapeutic alternative.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10268/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALamanda_silvaetal_IOC_2014.pdfapplication/pdf370738https://www.arca.fiocruz.br/bitstream/icict/10268/2/amanda_silvaetal_IOC_2014.pdf5646700743385decd1ba92ea14ffe4a7MD52TEXTamanda_silvaetal_IOC_2014.pdf.txtamanda_silvaetal_IOC_2014.pdf.txtExtracted texttext/plain18551https://www.arca.fiocruz.br/bitstream/icict/10268/3/amanda_silvaetal_IOC_2014.pdf.txt7d852a273a4ebbcb860d6ca6b3e6ed91MD53icict/102682022-06-24 13:10:55.165oai:www.arca.fiocruz.br:icict/10268TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:55Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
title RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
spellingShingle RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
Silva, Amanda Perse da
Herpes simples
Herpes simplex virus type 1
Real-time PCR
siRNA
RNA Interferente Pequeno
Herpes Simples
title_short RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
title_full RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
title_fullStr RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
title_full_unstemmed RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
title_sort RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions
author Silva, Amanda Perse da
author_facet Silva, Amanda Perse da
Lopes, Juliana Freitas
Paula, Vanessa Salete de
author_role author
author2 Lopes, Juliana Freitas
Paula, Vanessa Salete de
author2_role author
author
dc.contributor.author.fl_str_mv Silva, Amanda Perse da
Lopes, Juliana Freitas
Paula, Vanessa Salete de
dc.subject.other.pt_BR.fl_str_mv Herpes simples
topic Herpes simples
Herpes simplex virus type 1
Real-time PCR
siRNA
RNA Interferente Pequeno
Herpes Simples
dc.subject.en.pt_BR.fl_str_mv Herpes simplex virus type 1
Real-time PCR
siRNA
dc.subject.decs.pt_BR.fl_str_mv RNA Interferente Pequeno
Herpes Simples
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-08T14:08:46Z
dc.date.available.fl_str_mv 2015-05-08T14:08:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Amanda Perse da; LOPES, Juliana Freitas; PAULA, Vanessa Salete de. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions. The Brazilian Journal of Infectious Diseases, v.18, n.4, p.441-444, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10268
dc.identifier.issn.pt_BR.fl_str_mv 1413-8670
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.bjid.2014.01.011
identifier_str_mv SILVA, Amanda Perse da; LOPES, Juliana Freitas; PAULA, Vanessa Salete de. RNA interference inhibits herpes simplex virus type 1 isolated from saliva samples and mucocutaneous lesions. The Brazilian Journal of Infectious Diseases, v.18, n.4, p.441-444, 2014.
1413-8670
10.1016/j.bjid.2014.01.011
url https://www.arca.fiocruz.br/handle/icict/10268
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10268/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10268/2/amanda_silvaetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10268/3/amanda_silvaetal_IOC_2014.pdf.txt
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