Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16492 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
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Souza, Raquel SantosDiaz-Albiter, Hector ManuelDillon, Vivian MaureenDillon, Rod J.Genta, Fernando Ariel2016-12-08T16:47:07Z2016-12-08T16:47:07Z2016SOUZA, Raquel Santos; et al. Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations. Plos One, v.11, n.3, e0151403, 16p, Mar. 20161932-6203https://www.arca.fiocruz.br/handle/icict/1649210.1371/journal.pone.0151403engPublic Library of ScienceAedesLarvas de mosquitoProteínas de Saccharomyces cerevisiaeConsiderações fisiológicas e bioquímicasAedes aegyptiMosquito LarvaeYeastsBeta-1,3- glucanasesSaccharomyces cerevisiaePhysiological and Biochemical ConsiderationsDigestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.University of Liverpool. Biosciences Building. Institute of Integrative Biology. Liverpool, United Kingdom.Lancaster University. Furness Building. Division of Biomedical and Life Sciences. Bailrigg, Lancaster, United Kingdom / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Aedes aegypti larvae ingest several kinds of microorganisms. In spite of studies regarding mosquito digestion, little is known about the nutritional utilization of ingested cells by larvae. We investigated the effects of using yeasts as the sole nutrient source for A. aegypti larvae. We also assessed the role of beta-1,3-glucanases in digestion of live yeast cells. Beta-1,3-glucanases are enzymes which hydrolyze the cell wall beta-1,3-glucan polyssacharide. Larvae were fed with cat food (controls), live or autoclaved Saccharomyces cerevisiae cells and larval weight, time for pupation and adult emergence, larval and pupal mortality were measured. The presence of S. cerevisiae cells inside the larval gut was demonstrated by light microscopy. Beta-1,3-glucanase was measured in dissected larval samples. Viability assays were performed with live yeast cells and larval gut homogenates, with or without addition of competing beta-1,3-glucan. A. aegypti larvae fed with yeast cells were heavier at the 4th instar and showed complete development with normal mortality rates. Yeast cells were efficiently ingested by larvae and quickly killed (10% death in 2 h, 100% in 48 h). Larvae showed beta-1,3-glucanase in head, gut and rest of body. Gut beta-1,3-glucanase was not derived from ingested yeast cells. Gut and rest of body activity was not affected by the yeast diet, but head homogenates showed a lower activity in animals fed with autoclaved S. cerevisiae cells. The enzymatic lysis of live S. cerevisiae cells was demonstrated using gut homogenates, and this activity was abolished when excess beta-1,3-glucan was added to assays. These results show that live yeast cells are efficiently ingested and hydrolyzed by A. aegypti larvae, which are able to fully-develop on a diet based exclusively on these organisms. Beta-1,3-glucanase seems to be essential for yeast lytic activity of A. aegypti larvae, which possess significant amounts of these enzyme in all parts investigated.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16492/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfernando2_genta_etal_IOC_2016.pdffernando2_genta_etal_IOC_2016.pdfapplication/pdf645229https://www.arca.fiocruz.br/bitstream/icict/16492/2/fernando2_genta_etal_IOC_2016.pdf9e2ac179b2b84df8a327a34f1a90bab6MD52TEXTfernando2_genta_etal_IOC_2016.pdf.txtfernando2_genta_etal_IOC_2016.pdf.txtExtracted texttext/plain49545https://www.arca.fiocruz.br/bitstream/icict/16492/3/fernando2_genta_etal_IOC_2016.pdf.txt8e5eb991f9061735bd5cc00449ad51e3MD53icict/164922023-09-05 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| dc.title.pt_BR.fl_str_mv |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| title |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| spellingShingle |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations Souza, Raquel Santos Aedes Larvas de mosquito Proteínas de Saccharomyces cerevisiae Considerações fisiológicas e bioquímicas Aedes aegypti Mosquito Larvae Yeasts Beta-1,3- glucanases Saccharomyces cerevisiae Physiological and Biochemical Considerations |
| title_short |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| title_full |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| title_fullStr |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| title_full_unstemmed |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| title_sort |
Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations |
| author |
Souza, Raquel Santos |
| author_facet |
Souza, Raquel Santos Diaz-Albiter, Hector Manuel Dillon, Vivian Maureen Dillon, Rod J. Genta, Fernando Ariel |
| author_role |
author |
| author2 |
Diaz-Albiter, Hector Manuel Dillon, Vivian Maureen Dillon, Rod J. Genta, Fernando Ariel |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Raquel Santos Diaz-Albiter, Hector Manuel Dillon, Vivian Maureen Dillon, Rod J. Genta, Fernando Ariel |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes Larvas de mosquito Proteínas de Saccharomyces cerevisiae Considerações fisiológicas e bioquímicas |
| topic |
Aedes Larvas de mosquito Proteínas de Saccharomyces cerevisiae Considerações fisiológicas e bioquímicas Aedes aegypti Mosquito Larvae Yeasts Beta-1,3- glucanases Saccharomyces cerevisiae Physiological and Biochemical Considerations |
| dc.subject.en.pt_BR.fl_str_mv |
Aedes aegypti Mosquito Larvae Yeasts Beta-1,3- glucanases Saccharomyces cerevisiae Physiological and Biochemical Considerations |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2016 |
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2016-12-08T16:47:07Z |
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2016-12-08T16:47:07Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SOUZA, Raquel Santos; et al. Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations. Plos One, v.11, n.3, e0151403, 16p, Mar. 2016 |
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https://www.arca.fiocruz.br/handle/icict/16492 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0151403 |
| identifier_str_mv |
SOUZA, Raquel Santos; et al. Digestion of Yeasts and Beta-1,3-Glucanases in Mosquito Larvae: Physiological and Biochemical Considerations. Plos One, v.11, n.3, e0151403, 16p, Mar. 2016 1932-6203 10.1371/journal.pone.0151403 |
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https://www.arca.fiocruz.br/handle/icict/16492 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Public Library of Science |
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Public Library of Science |
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