Surfaceome of Leptospira spp.

Detalhes bibliográficos
Autor(a) principal: Cullen, Paul A
Data de Publicação: 2005
Outros Autores: Xu, Xiaoyi, Matsunaga, James, Sanchez, Yolanda, Ko, Albert Icksang, Haake, David A, Adler, Ben
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12130
Resumo: Australian Bacterial Pathogenesis Program and Victorian Bioinformatics Consortium. / Monash University. Department of Microbiology. Australia.
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spelling Cullen, Paul AXu, XiaoyiMatsunaga, JamesSanchez, YolandaKo, Albert IcksangHaake, David AAdler, Ben2015-11-06T12:15:16Z2015-11-06T12:15:16Z2005CULLEN, P. A. et al. Surfaceome of Leptospira spp. Infection and Immunity, v. 73, n. 8, p. 4853–4863, 2005.0019-9567https://www.arca.fiocruz.br/handle/icict/1213010.1128/IAI.73.8.4853–4863.2005engAmerican Society of MicrobiologyLeptospira sppLeptospiroseInfecçãoBactériaSurfaceome of Leptospira spp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAustralian Bacterial Pathogenesis Program and Victorian Bioinformatics Consortium. / Monash University. Department of Microbiology. Australia.Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, CaliforniaVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California / David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CaliforniaVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weil Medical College of Cornell University. Division of International Medicine and Infectious Diseases. New York, New YorkVeterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California / David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CaliforniaAustralian Bacterial Pathogenesis Program and Victorian Bioinformatics Consortium. / Monash University. Department of Microbiology. Australia.The identification of the subset of outer membrane proteins exposed on the surface of a bacterial cell (the surfaceome) is critical to understanding the interactions of bacteria with their environments and greatly narrows the search for protective antigens of extracellular pathogens. The surfaceome of Leptospira was investigated by biotin labeling of viable leptospires, affinity capture of the biotinylated proteins, two-dimensional gel electrophoresis, and mass spectrometry (MS). The leptospiral surfaceome was found to be predominantly made up of a small number of already characterized proteins, being in order of relative abundance on the cell surface: LipL32 > LipL21 > LipL41. Of these proteins, only LipL32 had not been previously identified as surface exposed. LipL32 surface exposure was subsequently verified by three independent approaches: surface immunofluorescence, whole-cell enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy. Three other proteins, Q8F8Q0 (a putative transmembrane outer membrane protein) and two proteins of 20 kDa and 55 kDa that could not be identified by MS, one of which demonstrated a high degree of labeling potentially representing an additional, as-yet-uncharacterized, surface-exposed protein. Minor labeling of p31LipL45, GroEL, and FlaB1 was also observed. Expression of the surfaceome constituents remained unchanged under a range of conditions investigated, including temperature and the presence of serum or urine. Immunization of mice with affinity-captured surface components stimulated the production of antibodies that bound surface proteins from heterologous leptospiral strains. The surfaceomics approach is particularly amenable to protein expression profiling using small amounts of sample (<107 cells) offering the potential to analyze bacterial surface expression during infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12130/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALCullen, PA Surfaceome of leptospira....pdfCullen, PA Surfaceome of leptospira....pdfapplication/pdf377430https://www.arca.fiocruz.br/bitstream/icict/12130/2/Cullen%2c%20PA%20Surfaceome%20of%20leptospira....pdf4b100bd35d34b0b4fcd06a466c05e3d8MD52TEXTCullen, PA Surfaceome of leptospira....pdf.txtCullen, PA Surfaceome of leptospira....pdf.txtExtracted texttext/plain54561https://www.arca.fiocruz.br/bitstream/icict/12130/3/Cullen%2c%20PA%20Surfaceome%20of%20leptospira....pdf.txt1f5aa3a142ec82266f9e845dba83537aMD53icict/121302023-03-15 14:33:21.736oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Surfaceome of Leptospira spp.
title Surfaceome of Leptospira spp.
spellingShingle Surfaceome of Leptospira spp.
Cullen, Paul A
Leptospira spp
Leptospirose
Infecção
Bactéria
title_short Surfaceome of Leptospira spp.
title_full Surfaceome of Leptospira spp.
title_fullStr Surfaceome of Leptospira spp.
title_full_unstemmed Surfaceome of Leptospira spp.
title_sort Surfaceome of Leptospira spp.
author Cullen, Paul A
author_facet Cullen, Paul A
Xu, Xiaoyi
Matsunaga, James
Sanchez, Yolanda
Ko, Albert Icksang
Haake, David A
Adler, Ben
author_role author
author2 Xu, Xiaoyi
Matsunaga, James
Sanchez, Yolanda
Ko, Albert Icksang
Haake, David A
Adler, Ben
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cullen, Paul A
Xu, Xiaoyi
Matsunaga, James
Sanchez, Yolanda
Ko, Albert Icksang
Haake, David A
Adler, Ben
dc.subject.other.pt_BR.fl_str_mv Leptospira spp
Leptospirose
Infecção
Bactéria
topic Leptospira spp
Leptospirose
Infecção
Bactéria
description Australian Bacterial Pathogenesis Program and Victorian Bioinformatics Consortium. / Monash University. Department of Microbiology. Australia.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2015-11-06T12:15:16Z
dc.date.available.fl_str_mv 2015-11-06T12:15:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CULLEN, P. A. et al. Surfaceome of Leptospira spp. Infection and Immunity, v. 73, n. 8, p. 4853–4863, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12130
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.73.8.4853–4863.2005
identifier_str_mv CULLEN, P. A. et al. Surfaceome of Leptospira spp. Infection and Immunity, v. 73, n. 8, p. 4853–4863, 2005.
0019-9567
10.1128/IAI.73.8.4853–4863.2005
url https://www.arca.fiocruz.br/handle/icict/12130
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv American Society of Microbiology
publisher.none.fl_str_mv American Society of Microbiology
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