Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics

Detalhes bibliográficos
Autor(a) principal: Ali, Nasir
Data de Publicação: 2017
Outros Autores: Rampazzo, Rita de Cássia Pontello, Costa, Alexandre Dias Tavares, Krieger, Marco Aurélio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20952
https://doi.org/10.1155/2017/9306564
Resumo: Universidade Federal do Paraná. Departamento de Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
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spelling Ali, NasirRampazzo, Rita de Cássia PontelloCosta, Alexandre Dias TavaresKrieger, Marco Aurélio2017-09-12T12:26:13Z2017-09-12T12:26:13Z2017ALI, Nasir et al. Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics. BioMed Research International, v. 2017, p. 1-13, 2017.2314-6141https://www.arca.fiocruz.br/handle/icict/20952https://doi.org/10.1155/2017/9306564porHindawi Publishing CorporationPOC-DxNucleic AcidsMolecular Sequence DataCell ExtractsÁcidos NucleicosDatos de Secuencia MolecularExtractos CelularesÁcidos NucleicosDados de Sequência MolecularExtratos CelularesCurrent Nucleic Acid extraction methods and their implications to point-of-care diagnosticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Nucleic acid extraction (NAE) plays a vital role in molecular biology as the primary step for many downstream applications. Many modifications have been introduced to the original 1869 method. Modern processes are categorized into chemical or mechanical, each with peculiarities that influence their use, especially in point-of-care diagnostics (POC-Dx). POC-Dx is a new approach aiming to replace sophisticated analytical machinery with microanalytical systems, able to be used near the patient, at the point of care or point of need. Although notable efforts have been made, a simple and effective extraction method is still a major challenge for widespread use of POC-Dx. In this review, we dissected the working principle of each of the most common NAE methods, overviewing their advantages and disadvantages, as well their potential for integration in POC-Dx systems. At present, it seems difficult, if not impossible, to establish a procedure which can be universally applied to POC-Dx. We also discuss the effects of the NAE chemicals upon the main plastic polymers used to mass produce POC-Dx systems. We end our review discussing the limitations and challenges that should guide the quest for an efficient extraction method that can be integrated in a POC-Dx system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20952/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALNasirCurrentNuc.pdfNasirCurrentNuc.pdfapplication/pdf1319786https://www.arca.fiocruz.br/bitstream/icict/20952/2/NasirCurrentNuc.pdfcc1a526713bc8ca6c156102cd76bed4eMD52TEXTNasirCurrentNuc.pdf.txtNasirCurrentNuc.pdf.txtExtracted texttext/plain70813https://www.arca.fiocruz.br/bitstream/icict/20952/3/NasirCurrentNuc.pdf.txt3c73e91e7db4d16c951faf594e1cf455MD53icict/209522018-08-15 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dc.title.pt_BR.fl_str_mv Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
title Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
spellingShingle Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
Ali, Nasir
POC-Dx
Nucleic Acids
Molecular Sequence Data
Cell Extracts
Ácidos Nucleicos
Datos de Secuencia Molecular
Extractos Celulares
Ácidos Nucleicos
Dados de Sequência Molecular
Extratos Celulares
title_short Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
title_full Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
title_fullStr Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
title_full_unstemmed Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
title_sort Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics
author Ali, Nasir
author_facet Ali, Nasir
Rampazzo, Rita de Cássia Pontello
Costa, Alexandre Dias Tavares
Krieger, Marco Aurélio
author_role author
author2 Rampazzo, Rita de Cássia Pontello
Costa, Alexandre Dias Tavares
Krieger, Marco Aurélio
author2_role author
author
author
dc.contributor.author.fl_str_mv Ali, Nasir
Rampazzo, Rita de Cássia Pontello
Costa, Alexandre Dias Tavares
Krieger, Marco Aurélio
dc.subject.other.pt_BR.fl_str_mv POC-Dx
topic POC-Dx
Nucleic Acids
Molecular Sequence Data
Cell Extracts
Ácidos Nucleicos
Datos de Secuencia Molecular
Extractos Celulares
Ácidos Nucleicos
Dados de Sequência Molecular
Extratos Celulares
dc.subject.en.pt_BR.fl_str_mv Nucleic Acids
Molecular Sequence Data
Cell Extracts
dc.subject.es.pt_BR.fl_str_mv Ácidos Nucleicos
Datos de Secuencia Molecular
Extractos Celulares
dc.subject.decs.pt_BR.fl_str_mv Ácidos Nucleicos
Dados de Sequência Molecular
Extratos Celulares
description Universidade Federal do Paraná. Departamento de Engenharia de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-09-12T12:26:13Z
dc.date.available.fl_str_mv 2017-09-12T12:26:13Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALI, Nasir et al. Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics. BioMed Research International, v. 2017, p. 1-13, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20952
dc.identifier.issn.pt_BR.fl_str_mv 2314-6141
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1155/2017/9306564
identifier_str_mv ALI, Nasir et al. Current Nucleic Acid extraction methods and their implications to point-of-care diagnostics. BioMed Research International, v. 2017, p. 1-13, 2017.
2314-6141
url https://www.arca.fiocruz.br/handle/icict/20952
https://doi.org/10.1155/2017/9306564
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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