A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52053 |
Resumo: | Universidade Estadual de Santa Cruz (UESC) |
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Santos, Edvan do CarmoMelo, Gabriela Imbassahy ValentimSantana, Paulo Vinícius BispoQuadros, Idaiara Graziele SilvaYahouédéhou, Sètondji Cocou Modeste AlexandreGuarda, Caroline Conceição daSantiago, Rayra PereiraFiuza, Luciana MagalhãesCarvalho, Suéllen PinheiroAdorno, Elisângela VitóriaKaneto, Carla MartinsFonseca, Teresa Cristina CardosoGoncalves, Marilda SouzaAleluia, Milena Magalhães2022-04-06T12:38:19Z2022-04-06T12:38:19Z2022SANTOS, Edvan do Carmo et. al. A description of the hemolytic component in sickle leg ulcer: The role of circulating miR-199a-5p, miR-144, and miR-126. Biomolecules, v. 12, n. 2, p. 1-10, 2022.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/5205310.3390/biom12020317Universidade Estadual de Santa Cruz (UESC)Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Laboratório de Patologia Aplicada e Genética. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Laboratório de Patologia Aplicada e Genética. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Laboratório de Patologia Aplicada e Genética. Ilhéus, BA, Brasil.Centro de Referência a Doença Falciforme de Itabuna. Itabuna, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Pesquisa em Anemias. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Pesquisa em Anemias. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Pesquisa em Anemias. Salvador, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Laboratório de Patologia Aplicada e Genética. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências da Saúde. Ilhéus, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Laboratório de Pesquisa em Anemias. Salvador, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Laboratório de Patologia Aplicada e Genética. Ilhéus, BA, Brasil.Sickle leg ulcers (SLU) are malleoli lesions with exuberant hemolytic pathophysiology. The microRNAs are potential genetic biomarkers for several pathologies. Thereby, we aimed to assess the expression of circulating miR-199a-5p, miR-144, and miR-126 in association with hemolytic biomarkers in SLU. This cross-sectional study included 69 patients with sickle cell disease, 52 patients without SLU (SLU-) and 17 patients with active SLU or previous history (SLU+). The results demonstrated elevated expression of circulating miR-199a-5p and miR-144 in SLU+ patients while miR-126 expression was reduced. Circulating miR-199a-5p and miR-144 were associated with hemolytic biomarkers such as LDH, indirect bilirubin, AST, GGT, iron, ferritin, RBC, hemoglobin, and NOm, in addition to association with impaired clinical profile of SLU. Furthermore, in silico analyses indicated interactions of miR-199a-5p with HIF1A, Ets-1, and TGFB2 genes, which are associated with vasculopathy and reduced NO. In contrast, miR-126 was associated with an attenuating clinical profile of SLU, in addition to not characterizing hemolysis. In summary, this study demonstrates, for the first time, that hemolytic mechanism in SLU can be characterized by circulating miR-199a-5p and miR-144. The circulating miR-126 may play a protective role in SLU. Thus, these microRNAs can support to establish prognosis and therapeutic strategy in SLU.engMDPIAnemia falciformeÚlcera da pernaMicroRNAsHemóliseBiomarcadoresAnemia, sickle cellLeg ulcerMicroRNAsHemolysisBiomarkersAnemia de células falciformesÚlcera de la piernaMicroARNsHemólisisBiomarcadoresDrépanocytoseUlcère de la jambemicroARNHémolyseMarqueurs biologiquesA description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52053/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos , Edvan do C. - A Description of the Hemolytic.pdfSantos , Edvan do C. - A Description of the Hemolytic.pdfapplication/pdf1375629https://www.arca.fiocruz.br/bitstream/icict/52053/2/Santos%20%2c%20Edvan%20do%20C.%20-%20A%20Description%20of%20the%20Hemolytic.pdfe96ac61cab672e27e55fe228817043e6MD52icict/520532023-03-15 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| dc.title.pt_BR.fl_str_mv |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| title |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| spellingShingle |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 Santos, Edvan do Carmo Anemia falciforme Úlcera da perna MicroRNAs Hemólise Biomarcadores Anemia, sickle cell Leg ulcer MicroRNAs Hemolysis Biomarkers Anemia de células falciformes Úlcera de la pierna MicroARNs Hemólisis Biomarcadores Drépanocytose Ulcère de la jambe microARN Hémolyse Marqueurs biologiques |
| title_short |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| title_full |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| title_fullStr |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| title_full_unstemmed |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| title_sort |
A description of the hemolytic component in sickle leg ulcer: the role of circulating miR-199a-5p, miR-144, and miR-126 |
| author |
Santos, Edvan do Carmo |
| author_facet |
Santos, Edvan do Carmo Melo, Gabriela Imbassahy Valentim Santana, Paulo Vinícius Bispo Quadros, Idaiara Graziele Silva Yahouédéhou, Sètondji Cocou Modeste Alexandre Guarda, Caroline Conceição da Santiago, Rayra Pereira Fiuza, Luciana Magalhães Carvalho, Suéllen Pinheiro Adorno, Elisângela Vitória Kaneto, Carla Martins Fonseca, Teresa Cristina Cardoso Goncalves, Marilda Souza Aleluia, Milena Magalhães |
| author_role |
author |
| author2 |
Melo, Gabriela Imbassahy Valentim Santana, Paulo Vinícius Bispo Quadros, Idaiara Graziele Silva Yahouédéhou, Sètondji Cocou Modeste Alexandre Guarda, Caroline Conceição da Santiago, Rayra Pereira Fiuza, Luciana Magalhães Carvalho, Suéllen Pinheiro Adorno, Elisângela Vitória Kaneto, Carla Martins Fonseca, Teresa Cristina Cardoso Goncalves, Marilda Souza Aleluia, Milena Magalhães |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Edvan do Carmo Melo, Gabriela Imbassahy Valentim Santana, Paulo Vinícius Bispo Quadros, Idaiara Graziele Silva Yahouédéhou, Sètondji Cocou Modeste Alexandre Guarda, Caroline Conceição da Santiago, Rayra Pereira Fiuza, Luciana Magalhães Carvalho, Suéllen Pinheiro Adorno, Elisângela Vitória Kaneto, Carla Martins Fonseca, Teresa Cristina Cardoso Goncalves, Marilda Souza Aleluia, Milena Magalhães |
| dc.subject.other.pt_BR.fl_str_mv |
Anemia falciforme Úlcera da perna MicroRNAs Hemólise Biomarcadores |
| topic |
Anemia falciforme Úlcera da perna MicroRNAs Hemólise Biomarcadores Anemia, sickle cell Leg ulcer MicroRNAs Hemolysis Biomarkers Anemia de células falciformes Úlcera de la pierna MicroARNs Hemólisis Biomarcadores Drépanocytose Ulcère de la jambe microARN Hémolyse Marqueurs biologiques |
| dc.subject.en.pt_BR.fl_str_mv |
Anemia, sickle cell Leg ulcer MicroRNAs Hemolysis Biomarkers |
| dc.subject.es.pt_BR.fl_str_mv |
Anemia de células falciformes Úlcera de la pierna MicroARNs Hemólisis Biomarcadores |
| dc.subject.fr.pt_BR.fl_str_mv |
Drépanocytose Ulcère de la jambe microARN Hémolyse Marqueurs biologiques |
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Universidade Estadual de Santa Cruz (UESC) |
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2022 |
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2022-04-06T12:38:19Z |
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2022-04-06T12:38:19Z |
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2022 |
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SANTOS, Edvan do Carmo et. al. A description of the hemolytic component in sickle leg ulcer: The role of circulating miR-199a-5p, miR-144, and miR-126. Biomolecules, v. 12, n. 2, p. 1-10, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52053 |
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2218-273X |
| dc.identifier.doi.none.fl_str_mv |
10.3390/biom12020317 |
| identifier_str_mv |
SANTOS, Edvan do Carmo et. al. A description of the hemolytic component in sickle leg ulcer: The role of circulating miR-199a-5p, miR-144, and miR-126. Biomolecules, v. 12, n. 2, p. 1-10, 2022. 2218-273X 10.3390/biom12020317 |
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