DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).

Detalhes bibliográficos
Autor(a) principal: Araújo, Amanda Domingues de
Data de Publicação: 2023
Outros Autores: Carvalho, Omar Dos Santos, Gava, Sandra Grossi, Caldeira, Roberta Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59605
Resumo: Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
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spelling Araújo, Amanda Domingues deCarvalho, Omar Dos SantosGava, Sandra GrossiCaldeira, Roberta Lima2023-07-14T18:24:06Z2023-07-14T18:24:06Z2023ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.11677872235-2988https://www.arca.fiocruz.br/handle/icict/59605engFrontiers Media SADNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHelminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Introduction: The genus Biomphalaria in Brazil includes 11 species and one subspecies, three of which are intermediate hosts of Schistosoma mansoni. Due to the recent evolution of this group, some species are difficult to identify based on morphological characters, making the use of genetic markers necessary for species identification. This study aimed to evaluate the use of partial sequences of the cytochrome c oxidase I (coi) gene for the identification of Biomphalaria species using phylogenetic reconstruction and species delimitation algorithms. The study tested the use of DNA barcoding technique for species delimitation within the genus. Methods: DNA barcoding was performed by sequencing a partial region of the coi gene from specimens, and the sequences were analyzed using phylogenetic reconstruction and algorithms to delimit Operational Taxonomic Units (OTUs). Results: The study found that the use of the coi gene in the reconstruction of the phylogeny of the genus might be an alternative for understanding the evolution and dispersion of species. However, this marker alone is not enough to solve complex taxonomic problems within the genus. A total of 223 sequences were analyzed, 102 of which could be separated using the barcode gap, enabling the correct identification of seven taxa. Discussion: The study demonstrated that accurate mollusk identification is necessary for effective schistosomiasis control. The DNA barcoding methodology was found to be promising for accurate mollusk identification, which is crucial for concentrating schistosomiasis control efforts in places where it is needed.DNA barcodemollusksBiomphalariamedical malacology collectionmolecular identificationcytochrome c oxidase coiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59605/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda_ Planorbidae).pdfDNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda_ Planorbidae).pdfapplication/pdf6740304https://www.arca.fiocruz.br/bitstream/icict/59605/2/DNA%20barcoding%20as%20a%20valuable%20tool%20for%20delimiting%20mollusk%20species%20of%20the%20genus%20Biomphalaria%20Preston%2c%201910%20%28Gastropoda_%20Planorbidae%29.pdfd57f015373cd0df0c6a31f4a0bdcdcd5MD52icict/596052023-07-14 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dc.title.en_US.fl_str_mv DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
title DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
spellingShingle DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
Araújo, Amanda Domingues de
DNA barcode
mollusks
Biomphalaria
medical malacology collection
molecular identification
cytochrome c oxidase coi
title_short DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
title_full DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
title_fullStr DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
title_full_unstemmed DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
title_sort DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
author Araújo, Amanda Domingues de
author_facet Araújo, Amanda Domingues de
Carvalho, Omar Dos Santos
Gava, Sandra Grossi
Caldeira, Roberta Lima
author_role author
author2 Carvalho, Omar Dos Santos
Gava, Sandra Grossi
Caldeira, Roberta Lima
author2_role author
author
author
dc.contributor.author.fl_str_mv Araújo, Amanda Domingues de
Carvalho, Omar Dos Santos
Gava, Sandra Grossi
Caldeira, Roberta Lima
dc.subject.en.en_US.fl_str_mv DNA barcode
mollusks
Biomphalaria
medical malacology collection
molecular identification
cytochrome c oxidase coi
topic DNA barcode
mollusks
Biomphalaria
medical malacology collection
molecular identification
cytochrome c oxidase coi
description Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-07-14T18:24:06Z
dc.date.available.fl_str_mv 2023-07-14T18:24:06Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.1167787
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59605
dc.identifier.issn.en_US.fl_str_mv 2235-2988
identifier_str_mv ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.1167787
2235-2988
url https://www.arca.fiocruz.br/handle/icict/59605
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59605/1/license.txt
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