Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46673 |
Resumo: | Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) – Protocol Number: 1094/2013-FAPEAM. • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). |
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Cozendey, Fernanda AnselmoSoumanou, Abdou GafarFerreira, Cleidiane de AguiarSobrinha, Flora Maia VigaCastro, Ana Caroline SantosBrito, Rafael OliveiraMota, Adolfo José daGonçalves, Marilda de SouzaMoura Neto, José Pereira de2021-04-14T11:21:33Z2021-04-14T11:21:33Z2021COZENDEY, Fernanda Anselmo et al. Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil. Mediterranean Journal of Hematology and Infectious Diseases, 2021.2035-3006https://www.arca.fiocruz.br/handle/icict/4667310.4084/MJHID.2021.001Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) – Protocol Number: 1094/2013-FAPEAM. • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).Universidade do Estado do Amazonas. Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil / Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Secretaria do Estado do Amazonas. Manaus, AM, Brasil.Secretaria do Estado do Amazonas. Manaus, AM, Brasil.Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Universidade do Estado do Amazonas. Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas. Manaus, AM, Brasil / Universidade Federal do Amazonas. Faculdade de Ciências Farmacêuticas. Manaus, AM, Brasil.Alpha Thalassemia (α-thal) is a heterogeneous group of hereditary alterations caused by deletions that affect alpha regulatory genes, and the 3.7Kb deletion is the most frequent worldwide. The prevalence ranges from 20% and 35% in Brazil, depending mainly on race, predominant in Afro-descendants. Purpose: The aim was to determine α-thal -α3.7Kb and -α4.2Kb deletions, estimating their frequency in individuals from six regions of Amazonas State. Methods: Volunteers age between 18-59 years old of both genders participated in the study. Blood was collected from March 2014 to September 2017 at the health centers of each participant city. α-thal3.7Kb was performed by GAP-PCR, while α-thal4.2Kb by Multiplex-PCR. The total samples collected from each city were: Manaus (capital), 356 (19.7%); Iranduba 232 (12.8%); Manacapuru, 287 (15.9%); Presidente Figueiredo, 370 (20.5%); Itacoatiara, 301 (16.6%); and Coari, 263 (14.5%). Results: The average age among males was 35.3±14.8, while for females, it was 36.7±14.9 years old. Microcytosis (MCV <80fL) was found in 158 individuals (8,46%) and α-thal diagnosed in 143 individuals (7.9%), and all of these individuals carried the 3.7Kb deletion 5.95% in heterozygous and 1.95% in homozygous. α-thal4.2kb was not found in any volunteer. The association analyses to the α-thal3.7kb genotypes were statistically significant for all hematological parameters (p<.001), except serum iron and serum ferritin analyses. Conclusion: This study highlights α-thal 3.7kb deletion as an important public health problem, especially in a population not yet characterized about this disease. Thus, epidemiological studies using molecular tools become relevant in regions where the disease is underestimated, contributing to a better understanding of thalassemia incidence and iron deficiency anemias incidence of the participating cities. We reinforce that future molecular studies in North Region from Brazil can be utilized to describe other genetic anemias as structural hemoglobinopathies that have already proven to be highly prevalent in Brazil.engPAGEpressAlpha TalassemiaPrevalênciaGrupos de Populações ContinentaisBrasilAlpha ThalassemiaIron TreatmentAmazonasBrazilDetection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/46673/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALAnselmo, Fernanda Cozendy, Detection of Common....pdfAnselmo, Fernanda Cozendy, Detection of Common....pdfapplication/pdf831260https://www.arca.fiocruz.br/bitstream/icict/46673/2/Anselmo%2c%20Fernanda%20Cozendy%2c%20Detection%20of%20Common....pdfe4d9e9f25f19ff6e9dd8777f5435228eMD52TEXTAnselmo, Fernanda Cozendy, Detection of Common....pdf.txtAnselmo, Fernanda Cozendy, Detection of Common....pdf.txtExtracted texttext/plain33218https://www.arca.fiocruz.br/bitstream/icict/46673/3/Anselmo%2c%20Fernanda%20Cozendy%2c%20Detection%20of%20Common....pdf.txt82772c2a98ea449a8f4aac359b984a52MD53icict/466732023-03-15 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| dc.title.pt_BR.fl_str_mv |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| title |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| spellingShingle |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil Cozendey, Fernanda Anselmo Alpha Talassemia Prevalência Grupos de Populações Continentais Brasil Alpha Thalassemia Iron Treatment Amazonas Brazil |
| title_short |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| title_full |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| title_fullStr |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| title_full_unstemmed |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| title_sort |
Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil |
| author |
Cozendey, Fernanda Anselmo |
| author_facet |
Cozendey, Fernanda Anselmo Soumanou, Abdou Gafar Ferreira, Cleidiane de Aguiar Sobrinha, Flora Maia Viga Castro, Ana Caroline Santos Brito, Rafael Oliveira Mota, Adolfo José da Gonçalves, Marilda de Souza Moura Neto, José Pereira de |
| author_role |
author |
| author2 |
Soumanou, Abdou Gafar Ferreira, Cleidiane de Aguiar Sobrinha, Flora Maia Viga Castro, Ana Caroline Santos Brito, Rafael Oliveira Mota, Adolfo José da Gonçalves, Marilda de Souza Moura Neto, José Pereira de |
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author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cozendey, Fernanda Anselmo Soumanou, Abdou Gafar Ferreira, Cleidiane de Aguiar Sobrinha, Flora Maia Viga Castro, Ana Caroline Santos Brito, Rafael Oliveira Mota, Adolfo José da Gonçalves, Marilda de Souza Moura Neto, José Pereira de |
| dc.subject.other.pt_BR.fl_str_mv |
Alpha Talassemia Prevalência Grupos de Populações Continentais Brasil |
| topic |
Alpha Talassemia Prevalência Grupos de Populações Continentais Brasil Alpha Thalassemia Iron Treatment Amazonas Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Alpha Thalassemia Iron Treatment Amazonas Brazil |
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Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) – Protocol Number: 1094/2013-FAPEAM. • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). |
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2021 |
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2021-04-14T11:21:33Z |
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2021-04-14T11:21:33Z |
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2021 |
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COZENDEY, Fernanda Anselmo et al. Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil. Mediterranean Journal of Hematology and Infectious Diseases, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46673 |
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2035-3006 |
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10.4084/MJHID.2021.001 |
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COZENDEY, Fernanda Anselmo et al. Detection of Common Deletional of α-Thalassemia 3.7 Kb from Metropolitan Region of Manaus, Amazonas, Brazil. Mediterranean Journal of Hematology and Infectious Diseases, 2021. 2035-3006 10.4084/MJHID.2021.001 |
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