Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil

Bibliographic Details
Main Author: Carramaschi, Isabel N.
Publication Date: 2021
Other Authors: Ferreira, Vítor dos S. B., Chagas, Thiago P.G., Corrêa, Lais L., Picão, Renata C, Queiroz, Margareth M. de C., Rangel, Karyne, Jardim, Rodrigo, Mota, Fabio F. da, Zahner, Viviane
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/47859
Summary: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
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spelling Carramaschi, Isabel N.Ferreira, Vítor dos S. B.Chagas, Thiago P.G.Corrêa, Lais L.Picão, Renata C,Queiroz, Margareth M. de C.Rangel, KaryneJardim, RodrigoMota, Fabio F. daZahner, Viviane2021-06-24T18:33:20Z2021-06-24T18:33:20Z2021CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021.2213-7165https://www.arca.fiocruz.br/handle/icict/4785910.1016/j.jgar.2020.11.021engScience DirectKlebsiella quasipneumoniaeMoscasSaúde PúblicaResistência a antibióticosKlebsiella quasipneumoniaeFliesPublic healthAntibiotic resistanceMultidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Computacional e Sistemas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.Objectives: Flies have been implicated in the dispersal of medically important bacteria including members of the genus Klebsiella between different environmental compartments. The aim of this study was to retrieve and characterize antibiotic-resistant bacteria from flies collected near to hospitals. Methods: Flies were collected in the vicinity of medical facilities and examined for bacteria demonstrating phenotypic resistance to ceftriaxone, followed by determination of phenotypic and genotypic resistance profiles. In addition, whole genome sequencing followed by phylogenetic analysis and resistance genotyping were performed with the multidrug-resistant (MDR) strain Lemef23, identified as Klebsiella quasipneumoniae subsp. similipneumoniae. Results: The strain Lemef23, classified by multiple locus sequence typing as novel ST 3397, harboured numerous resistance genes. The blaNDM was located on a Tn3000 element, a common genetic platform for the carriage of this gene in Brazil. Inference of phylogenetic orthology of strain Lemef23 and other clinical isolates suggested an anthropogenic origin. Conclusions: The findings of this study support the role of flies as vectors of MDR bacteria of clinical importance and provide the first record of blaNDM-1 and blaCTXM-15 in a Brazilian isolate of K. quasipneumoniae subsp. similipneumoniae, demonstrating the value of surveying insects as reservoirs of antibiotic resistance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47859/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALVivianeZaher_etal_IOC_2021.pdfVivianeZaher_etal_IOC_2021.pdfapplication/pdf755465https://www.arca.fiocruz.br/bitstream/icict/47859/2/VivianeZaher_etal_IOC_2021.pdf3e1f0912ea49f6fd00f1df8f5305a586MD52TEXTVivianeZaher_etal_IOC_2021.pdf.txtVivianeZaher_etal_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
title Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
spellingShingle Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
Carramaschi, Isabel N.
Klebsiella quasipneumoniae
Moscas
Saúde Pública
Resistência a antibióticos
Klebsiella quasipneumoniae
Flies
Public health
Antibiotic resistance
title_short Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
title_full Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
title_fullStr Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
title_full_unstemmed Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
title_sort Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil
author Carramaschi, Isabel N.
author_facet Carramaschi, Isabel N.
Ferreira, Vítor dos S. B.
Chagas, Thiago P.G.
Corrêa, Lais L.
Picão, Renata C,
Queiroz, Margareth M. de C.
Rangel, Karyne
Jardim, Rodrigo
Mota, Fabio F. da
Zahner, Viviane
author_role author
author2 Ferreira, Vítor dos S. B.
Chagas, Thiago P.G.
Corrêa, Lais L.
Picão, Renata C,
Queiroz, Margareth M. de C.
Rangel, Karyne
Jardim, Rodrigo
Mota, Fabio F. da
Zahner, Viviane
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carramaschi, Isabel N.
Ferreira, Vítor dos S. B.
Chagas, Thiago P.G.
Corrêa, Lais L.
Picão, Renata C,
Queiroz, Margareth M. de C.
Rangel, Karyne
Jardim, Rodrigo
Mota, Fabio F. da
Zahner, Viviane
dc.subject.other.pt_BR.fl_str_mv Klebsiella quasipneumoniae
Moscas
Saúde Pública
Resistência a antibióticos
topic Klebsiella quasipneumoniae
Moscas
Saúde Pública
Resistência a antibióticos
Klebsiella quasipneumoniae
Flies
Public health
Antibiotic resistance
dc.subject.en.pt_BR.fl_str_mv Klebsiella quasipneumoniae
Flies
Public health
Antibiotic resistance
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Entomologia Médica e Forense. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-24T18:33:20Z
dc.date.available.fl_str_mv 2021-06-24T18:33:20Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47859
dc.identifier.issn.pt_BR.fl_str_mv 2213-7165
dc.identifier.doi.none.fl_str_mv 10.1016/j.jgar.2020.11.021
identifier_str_mv CARRAMASCHI, Isabel N. et al. Multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae carrying blaNDM-blaCTX-M15 isolated from flies in Rio de Janeiro, Brazil. Journal of Global Antimicrobial Resistance, v. 24, p. 1-5, 2021.
2213-7165
10.1016/j.jgar.2020.11.021
url https://www.arca.fiocruz.br/handle/icict/47859
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Science Direct
publisher.none.fl_str_mv Science Direct
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/47859/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/47859/2/VivianeZaher_etal_IOC_2021.pdf
https://www.arca.fiocruz.br/bitstream/icict/47859/3/VivianeZaher_etal_IOC_2021.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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