Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/49425 |
Resumo: | Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil. |
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Silva, Vanessa S.Skueresky, NatáliaLopes, FernandoKoch, Tabata K.Ott, Paulo HenriqueSiciliano, SalvatoreBarreto, André S.Secchi, Eduardo R.Meirelles, Ana Carolina O. deCarvalho, Vitor LuzBorges, João C. G.Danilewicz, DanielFarro, Ana Paula C.Barbosa, Lupércio A.Martins Jr., S. JoséDomit, CamilaSerrano, InêsSilva, TiagoTrinca, CristineMamontel, MiriamEmin-Lima, Neusa RenataVliati, Victor HugoEizirik, EduardoOliveira, Larissa Rosa de2021-10-18T14:41:19Z2021-10-18T14:41:19Z2021SILVA, Vanessa S. et al. Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil. Mammal Research, On Line, p. 1-21, Feb. 2021.2199-2401https://www.arca.fiocruz.br/handle/icict/4942510.1007/s13364-021-00555-wengSpringerBaleiasGolfinhosEncalheMorfológicoIdentificaçãoMolecularWhalesDolphinsStrandingMorphologicalIdentificationMolecularIntegrating morphology and DNA barcoding to assess cetacean diversity in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil.Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil.Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil.Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil.Universidade Estadual do Rio Grande do Sul. Porto Alegre, RS, Brasil / Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul (GEMARS). Tôrres, RS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade do Vale do Itajaí. Itajaí, SC, Brasil.Universidade Federal do Rio Grande. Rio Grande, RS, Brasil.Associação de Pesquisa e Preservação de Ecossistemas Aquáticos. Caucaia, CE, Brasil.Associação de Pesquisa e Preservação de Ecossistemas Aquáticos. Caucaia, CE, Brasil.Fundação Mamíferos Aquáticos. São Cristóvão, SE, Brasil.Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul. Tôrres, RS, Brasil,Universidade Federal do Espírito Santo. Departamento de Ciências Agrárias e Biológicas. São Matheus, ES, Brasil.Organização Consciência Ambiental. Guarapari, ES, Brasil.Instituto Chico Mendes de Conservação da Biodiversidade/Projeto Golfinho Rotador. Fernando de Noronha, PE, Brasil.Universidade Federal do Paraná. Centro de Estudos do Mar. Pontal do Paraná, PR, Brasil.Instituto Chico Mendes de Conservação da Biodiversidade. Estrada Estadual Bocaina, SP, Brasil.Pontifícia Universidade Católica do Rio Grande do Sul. Porto Alegre, RS, Brasil.Pontifícia Universidade Católica do Rio Grande do Sul. Porto Alegre, RS, Brasil.Instituto de Desenvolvimento Sustentável Mamirauá. Tefé, AM, Brasil.Museu Paraense Emílio Goeldi, Grupo de Estudos de Mamíferos Aquáticos da Amazônia. Belém, PA, Brasil.Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil.Pontifícia Universidade Católica do Rio Grande do Sul. Porto Alegre, RS, Brasil.Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil / Grupo de Estudos de Mamíferos Aquáticos do Rio Grande do Sul. Tôrres, RS, Brasil.Stranded cetaceans (whales, dolphins, and porpoises) are frequently used to obtain data on species occurrence and demographic trends. Accurate species-level identification of these individuals is crucial, but often challenging or impossible when relying solely on morphological features (e.g., for highly decayed specimens). To aid in the development of a reliable molecular assay for cetacean DNA-based identification, we tested the efficacy of the standardized DNA barcode segment of the coxI gene in identifying cetaceans occurring off the Brazilian coast and in its continental waters. We generated coxI sequences from 150 specimens (collected by 16 Brazilian institutions), most of which included voucher material (skulls, skeletons and/or images) deposited in scientific collections. This allowed a direct comparison between their morphological and molecular identification. CoxI sequences correctly identified ~93% of the samples, comprising 33 species (70% of the 47 cetaceans reported for Brazilian waters). Two species (Berardius arnuxii and Phocoena dioptrica) were sequenced for coxI for the first time. For only two dolphins (Stenella coeruleoalba and S. clymene) and a right whale (Eubalaena australis), coxI failed to identify the species due to overlapping distributions of intra- vs. interspecific divergences. Only one right whale species occurs in the southern hemisphere, facilitating identification in this case. Stenella dolphins present extensive sympatry and potential inter-species hybridization, suggesting that nuclear markers may be required for their reliable identification. These results indicate that DNA barcoding can reliably identify most stranded cetaceans and highlight the importance of voucher materials to validate the construction of a reliable DNA-based identification system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/49425/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSalvatore_Siciliano_IOC_etal_2021.pdfSalvatore_Siciliano_IOC_etal_2021.pdfapplication/pdf4530187https://www.arca.fiocruz.br/bitstream/icict/49425/2/Salvatore_Siciliano_IOC_etal_2021.pdfa5d7cd3d01dced2b66aa7e447e231565MD52icict/494252021-10-20 10:09:46.114oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-10-20T13:09:46Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.pt_BR.fl_str_mv |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| title |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| spellingShingle |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil Silva, Vanessa S. Baleias Golfinhos Encalhe Morfológico Identificação Molecular Whales Dolphins Stranding Morphological Identification Molecular |
| title_short |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| title_full |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| title_fullStr |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| title_full_unstemmed |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| title_sort |
Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil |
| author |
Silva, Vanessa S. |
| author_facet |
Silva, Vanessa S. Skueresky, Natália Lopes, Fernando Koch, Tabata K. Ott, Paulo Henrique Siciliano, Salvatore Barreto, André S. Secchi, Eduardo R. Meirelles, Ana Carolina O. de Carvalho, Vitor Luz Borges, João C. G. Danilewicz, Daniel Farro, Ana Paula C. Barbosa, Lupércio A. Martins Jr., S. José Domit, Camila Serrano, Inês Silva, Tiago Trinca, Cristine Mamontel, Miriam Emin-Lima, Neusa Renata Vliati, Victor Hugo Eizirik, Eduardo Oliveira, Larissa Rosa de |
| author_role |
author |
| author2 |
Skueresky, Natália Lopes, Fernando Koch, Tabata K. Ott, Paulo Henrique Siciliano, Salvatore Barreto, André S. Secchi, Eduardo R. Meirelles, Ana Carolina O. de Carvalho, Vitor Luz Borges, João C. G. Danilewicz, Daniel Farro, Ana Paula C. Barbosa, Lupércio A. Martins Jr., S. José Domit, Camila Serrano, Inês Silva, Tiago Trinca, Cristine Mamontel, Miriam Emin-Lima, Neusa Renata Vliati, Victor Hugo Eizirik, Eduardo Oliveira, Larissa Rosa de |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Vanessa S. Skueresky, Natália Lopes, Fernando Koch, Tabata K. Ott, Paulo Henrique Siciliano, Salvatore Barreto, André S. Secchi, Eduardo R. Meirelles, Ana Carolina O. de Carvalho, Vitor Luz Borges, João C. G. Danilewicz, Daniel Farro, Ana Paula C. Barbosa, Lupércio A. Martins Jr., S. José Domit, Camila Serrano, Inês Silva, Tiago Trinca, Cristine Mamontel, Miriam Emin-Lima, Neusa Renata Vliati, Victor Hugo Eizirik, Eduardo Oliveira, Larissa Rosa de |
| dc.subject.other.pt_BR.fl_str_mv |
Baleias Golfinhos Encalhe Morfológico Identificação Molecular |
| topic |
Baleias Golfinhos Encalhe Morfológico Identificação Molecular Whales Dolphins Stranding Morphological Identification Molecular |
| dc.subject.en.pt_BR.fl_str_mv |
Whales Dolphins Stranding Morphological Identification Molecular |
| description |
Universidade do Vale do Rio dos Sinos. São Leopoldo, RS, Brasil. |
| publishDate |
2021 |
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2021-10-18T14:41:19Z |
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2021-10-18T14:41:19Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SILVA, Vanessa S. et al. Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil. Mammal Research, On Line, p. 1-21, Feb. 2021. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/49425 |
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2199-2401 |
| dc.identifier.doi.none.fl_str_mv |
10.1007/s13364-021-00555-w |
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SILVA, Vanessa S. et al. Integrating morphology and DNA barcoding to assess cetacean diversity in Brazil. Mammal Research, On Line, p. 1-21, Feb. 2021. 2199-2401 10.1007/s13364-021-00555-w |
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Springer |
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Springer |
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