Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum

Detalhes bibliográficos
Autor(a) principal: Muniz, Mauro de Medeiros
Data de Publicação: 2010
Outros Autores: Tavares, Patrícia Morais e Silva, Meyer, Wieland, Nosanchuk, Joshua Daniel, Zancope-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32206
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Muniz, Mauro de MedeirosTavares, Patrícia Morais e SilvaMeyer, WielandNosanchuk, Joshua DanielZancope-Oliveira, Rosely Maria2019-03-21T23:12:43Z2019-03-21T23:12:43Z2010MUNIZ, Mauro de Medeiros et al. Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum. Applied and Environmental Microbiology, v. 76, n. 13, p. 4438-4447, July 2010.0099-2240https://www.arca.fiocruz.br/handle/icict/3220610.1128/AEM.02004-091098-5336engAmerican Society for MicrobiologyComparison of different DNA-based methods for molecular typing of Histoplasma capsulatuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. New South Wales, Australia / Westmead Hospital. New South Wales, Australia.Albert Einstein College of Medicine. Microbiology and Immunology. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with different methods using the same set of isolates. To explore the diversity of H. capsulatum in Brazil and to determine correlations between the results of three different molecular typing techniques, we examined 51 environmental, animal, and human isolates by M13 PCR fingerprinting, PCR-restriction fragment length polymorphism (RFLP) analysis of the internal transcribed region 1 (ITS1)-5.8S-ITS2 region of the rDNA locus, and DNA sequencing and phylogenetic analysis of parts of four protein-encoding genes, the Arf (ADP ribosylation factor), H-anti (H antigen precursor), Ole (delta-9 fatty acid desaturase), and Tub1 (alpha-tubulin) genes. Each method identified three major genetic clusters, and there was a high level of concordance between the results of the typing techniques. The M13 PCR fingerprinting and PCR-RFLP analyses produced very similar results and separated the H. capsulatum isolates included in this study into three major groups. An additional approach used was comparison of our Brazilian ITS1-5.8S-ITS2 sequences with the sequences deposited previously in NCBI data banks. Our analyses suggest that H. capsulatum can be divided into different molecular types that are dispersed around the world. Our results indicate that the three methods used in this study are reliable and reproducible and that they have similar sensitivities. However, M13 PCR fingerprinting has some advantages over the other two methods as it is faster, cheaper, and more user friendly, which especially increases its utility for molecular typing of Histoplasma in situations where laboratory facilities are relatively limited.Histoplasma capsulatumMolecular typing techniquesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32206/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Muniz_Mauro_etal_INI_2010.pdfve_Muniz_Mauro_etal_INI_2010.pdfapplication/pdf1298614https://www.arca.fiocruz.br/bitstream/icict/32206/2/ve_Muniz_Mauro_etal_INI_2010.pdfe9db0f1cbe38ec8d24467f8116ecda64MD52TEXTve_Muniz_Mauro_etal_INI_2010.pdf.txtve_Muniz_Mauro_etal_INI_2010.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
title Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
spellingShingle Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
Muniz, Mauro de Medeiros
Histoplasma capsulatum
Molecular typing techniques
title_short Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
title_full Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
title_fullStr Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
title_full_unstemmed Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
title_sort Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum
author Muniz, Mauro de Medeiros
author_facet Muniz, Mauro de Medeiros
Tavares, Patrícia Morais e Silva
Meyer, Wieland
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
author_role author
author2 Tavares, Patrícia Morais e Silva
Meyer, Wieland
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Muniz, Mauro de Medeiros
Tavares, Patrícia Morais e Silva
Meyer, Wieland
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
Molecular typing techniques
topic Histoplasma capsulatum
Molecular typing techniques
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-03-21T23:12:43Z
dc.date.available.fl_str_mv 2019-03-21T23:12:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MUNIZ, Mauro de Medeiros et al. Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum. Applied and Environmental Microbiology, v. 76, n. 13, p. 4438-4447, July 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32206
dc.identifier.issn.pt_BR.fl_str_mv 0099-2240
dc.identifier.doi.none.fl_str_mv 10.1128/AEM.02004-09
dc.identifier.eissn.none.fl_str_mv 1098-5336
identifier_str_mv MUNIZ, Mauro de Medeiros et al. Comparison of different DNA-based methods for molecular typing of Histoplasma capsulatum. Applied and Environmental Microbiology, v. 76, n. 13, p. 4438-4447, July 2010.
0099-2240
10.1128/AEM.02004-09
1098-5336
url https://www.arca.fiocruz.br/handle/icict/32206
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32206/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32206/2/ve_Muniz_Mauro_etal_INI_2010.pdf
https://www.arca.fiocruz.br/bitstream/icict/32206/3/ve_Muniz_Mauro_etal_INI_2010.pdf.txt
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