Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population

Detalhes bibliográficos
Autor(a) principal: Jesus, Camila P. de
Data de Publicação: 2020
Outros Autores: Dias, Fernando B. S., Villela, Daniel M. A., Freitas, Rafael Maciel de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42554
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
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spelling Jesus, Camila P. deDias, Fernando B. S.Villela, Daniel M. A.Freitas, Rafael Maciel de2020-08-05T18:42:19Z2020-08-05T18:42:19Z2020JESUS, Camila P. de et al. Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population. Insects, v. 11, n. 92, 12p, 2020.2075-4450https://www.arca.fiocruz.br/handle/icict/4255410.3390/insects11020092engMDPIAedes aegyptiWolbachiaOvitrapFrequência de amostragemBG-SentinelAedes aegyptiOvitrapWolbachiaBG-SentinelSamplingOvitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Populationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Gabinete da Presidência. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Deployment of Aedes aegypti mosquitoes carrying the endosymbiont bacterium Wolbachia has been identified as a promising strategy to reduce dengue, chikungunya, and Zika transmission. We investigated whether sampling larvae from ovitraps can provide reliable estimates on Wolbachia frequency during releases, as compared to the expensive adult-based BG-Sentinel. We conducted pilot releases in a semi-field system (SFS) divided into six cages of 21 m2, each with five ovitraps. Five treatments were chosen to represent different points of a hypothetical invasion curve: 10%, 25%, 50%, 75%, and 90% of Wolbachia frequency. Collected eggs were counted and hatched, and the individuals from a net sample of 27% of larvae per treatment were screened for Wolbachia presence by RT-qPCR. Ovitrap positioning had no effect on egg hatching rate. Treatment strongly affected the number of eggs collected and also the hatching rate, especially when Wolbachia was at a 10% frequency. A second observation was done during the release of Wolbachia in Rio under a population replacement approach when bacterium frequency was estimated using 30 BG-Sentinel traps and 45 ovitraps simultaneously. By individually screening 35% (N = 3904) of larvae collected by RT-qPCR, we were able to produce a similar invasion curve to the one observed when all adults were individually screened. If sampling is reduced to 20%, monitoring Wolbachia frequency with 45 ovitraps would be roughly half the cost of screening all adult mosquitoes captured by 30 BG-Sentinels. Our findings support the scale-up of Wolbachia releases, especially in areas with limited resources to afford massive trapping with BG-Sentinel traps.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42554/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdfCamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdfapplication/pdf2110962https://www.arca.fiocruz.br/bitstream/icict/42554/2/CamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdffeac6021991cb9a532c3f54fc3700552MD52TEXTCamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdf.txtCamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdf.txtExtracted texttext/plain49991https://www.arca.fiocruz.br/bitstream/icict/42554/3/CamilaPJesus_RafaelMFreitas_etal_IOC_2020.pdf.txt7865780cd1ea14410c025e0ed269e279MD53icict/425542020-08-06 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dc.title.pt_BR.fl_str_mv Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
title Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
spellingShingle Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
Jesus, Camila P. de
Aedes aegypti
Wolbachia
Ovitrap
Frequência de amostragem
BG-Sentinel
Aedes aegypti
Ovitrap
Wolbachia
BG-Sentinel
Sampling
title_short Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
title_full Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
title_fullStr Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
title_full_unstemmed Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
title_sort Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population
author Jesus, Camila P. de
author_facet Jesus, Camila P. de
Dias, Fernando B. S.
Villela, Daniel M. A.
Freitas, Rafael Maciel de
author_role author
author2 Dias, Fernando B. S.
Villela, Daniel M. A.
Freitas, Rafael Maciel de
author2_role author
author
author
dc.contributor.author.fl_str_mv Jesus, Camila P. de
Dias, Fernando B. S.
Villela, Daniel M. A.
Freitas, Rafael Maciel de
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Wolbachia
Ovitrap
Frequência de amostragem
BG-Sentinel
topic Aedes aegypti
Wolbachia
Ovitrap
Frequência de amostragem
BG-Sentinel
Aedes aegypti
Ovitrap
Wolbachia
BG-Sentinel
Sampling
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Ovitrap
Wolbachia
BG-Sentinel
Sampling
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-05T18:42:19Z
dc.date.available.fl_str_mv 2020-08-05T18:42:19Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv JESUS, Camila P. de et al. Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population. Insects, v. 11, n. 92, 12p, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42554
dc.identifier.issn.pt_BR.fl_str_mv 2075-4450
dc.identifier.doi.none.fl_str_mv 10.3390/insects11020092
identifier_str_mv JESUS, Camila P. de et al. Ovitraps Provide a Reliable Estimate of Wolbachia Frequency during wMelBr Strain Deployment in a Geographically Isolated Aedes aegypti Population. Insects, v. 11, n. 92, 12p, 2020.
2075-4450
10.3390/insects11020092
url https://www.arca.fiocruz.br/handle/icict/42554
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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