Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status

Detalhes bibliográficos
Autor(a) principal: Souza, Daniel Menezes
Data de Publicação: 2011
Outros Autores: Oliveira, Rodrigo Corrêa de, Sá, Renata Guerra, Giunchetti, Rodolfo Cordeiro, Carvalho, Andréa Teixeira de, Martins Filho, Olindo Assis, Oliveira, Guilherme Corrêa de, Reis, Alexandre Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8962
Resumo: O artigo encontra-se disponível em acesso aberto no site do Editor.
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spelling Souza, Daniel MenezesOliveira, Rodrigo Corrêa deSá, Renata GuerraGiunchetti, Rodolfo CordeiroCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisOliveira, Guilherme Corrêa deReis, Alexandre Barbosa2014-11-25T17:13:49Z2014-11-25T17:13:49Z2011SOUZA, Daniel Menezes et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status. Veterinary Parasitology, v. 177, n. 1/2, p. 39-49, 2011.0304-4017https://www.arca.fiocruz.br/handle/icict/896210.1016/j.vetpar.2010.11.025O artigo encontra-se disponível em acesso aberto no site do Editor.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, Minas Gerais, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, Minas Gerais, Brasil.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brasil.The immune response in the skin of dogs infected with Leishmania chagasi and its association with distinct levels of tissue parasitism and clinical progression of canine visceral leishmaniasis (CVL) are poorly understood and limited studies are available. A detailed analysis of the profiles of cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-12, IL-13, TGF-β1 and TNF-α) and transcription factors (T-bet, GATA-3 and FOXP3) in the skin of 35 naturally infected dogs was carried out using real-time PCR alongside determinations of skin parasite density and the clinical status of CVL. A mixed cytokine profile with high levels of expression of IFN-γ, TNF-α and IL-13 was determined in asymptomatic dogs. Additionally, the levels of transcription factors GATA-3 and FOXP3 were correlated with the asymptomatic disease. A mixed cytokine profile was also observed during active CVL. Moreover, high levels of IL-10 and TGF-β1, concomitant with the low expression of IL-12, may represent a key condition that allows persistence of parasite replication in the skin. The results obtained indicate that in asymptomatic disease or lower levels of skin parasite density, a mixed inflammatory, regulatory immune response profile may be of major relevance for both the maintenance of the clinical status of the dogs as well as for parasite persistence and replication at low levels.engElsevier Ltda.Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical statusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCanine visceral leishmaniasisCytokinesTranscription factorsReal-time PCRLeishmania chagasiParasitisminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8962/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALCytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.pdfCytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.pdfapplication/pdf703891https://www.arca.fiocruz.br/bitstream/icict/8962/2/Cytokine%20and%20transcription%20factor%20profiles%20in%20the%20skin%20of%20dogs%20naturally%20infected%20by%20Leishmania%20%28Leishmania%29%20chagasi%20presenting%20distinct%20cutaneous%20parasite%20density%20and%20clinical%20status.pdfe9ce6ec17d6aa000759ec90dafd1c48fMD52TEXTCytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status.pdf.txtCytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania 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dc.title.pt_BR.fl_str_mv Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
title Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
spellingShingle Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
Souza, Daniel Menezes
Canine visceral leishmaniasis
Cytokines
Transcription factors
Real-time PCR
Leishmania chagasi
Parasitism
title_short Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
title_full Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
title_fullStr Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
title_full_unstemmed Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
title_sort Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status
author Souza, Daniel Menezes
author_facet Souza, Daniel Menezes
Oliveira, Rodrigo Corrêa de
Sá, Renata Guerra
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
author_role author
author2 Oliveira, Rodrigo Corrêa de
Sá, Renata Guerra
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Daniel Menezes
Oliveira, Rodrigo Corrêa de
Sá, Renata Guerra
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Oliveira, Guilherme Corrêa de
Reis, Alexandre Barbosa
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Cytokines
Transcription factors
Real-time PCR
Leishmania chagasi
Parasitism
topic Canine visceral leishmaniasis
Cytokines
Transcription factors
Real-time PCR
Leishmania chagasi
Parasitism
description O artigo encontra-se disponível em acesso aberto no site do Editor.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-25T17:13:49Z
dc.date.available.fl_str_mv 2014-11-25T17:13:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Daniel Menezes et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status. Veterinary Parasitology, v. 177, n. 1/2, p. 39-49, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8962
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetpar.2010.11.025
identifier_str_mv SOUZA, Daniel Menezes et al. Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct skin parasite density and clinical status. Veterinary Parasitology, v. 177, n. 1/2, p. 39-49, 2011.
0304-4017
10.1016/j.vetpar.2010.11.025
url https://www.arca.fiocruz.br/handle/icict/8962
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltda.
publisher.none.fl_str_mv Elsevier Ltda.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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