Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28775 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
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Oliveira, Warllem JunioMagalhaes, Fernanda do CarmoElias, Andressa Mariana SaldanhaCastro, Vanessa Normandio deFavero, VivianLindholz, Catieli GobettiOliveira, Aureo AlmeidaBarbosa, Fernando SergioGil, FredericoGomes, Maria AparecidaTeixeira, Carlos GraeffEnk, Martin JohannesCoelho, Paulo Marcos ZechCarneiro, MariangelaCorrea, Deborah Aparecida NegraoGeiger, Stefan Michael2018-09-13T19:04:22Z2018-09-13T19:04:22Z2018OLIVEIRA, Warllem Junio et al. Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test. PLoS Negl Trop Dis., v. 12, n. 2, e0006232, 20181935-2735https://www.arca.fiocruz.br/handle/icict/2877510.1371/journal.pntd.0006232engPublic Library of ScienceEsquistossomoseSchistosomiasisEvaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine testinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilPontifıcia Universidade Catolica de Rio Grande do Sul. Grupo de Parasitologia Medica. Porto Alegre, RS, BrasilPontifıcia Universidade Catolica de Rio Grande do Sul. Grupo de Parasitologia Medica. Porto Alegre, RS, BrasilFundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilPontifıcia Universidade Catolica de Rio Grande do Sul. Grupo de Parasitologia Medica. Porto Alegre, RS, BrasilMinisterio da Saude. Instituto Evandro Chagas. Secretaria de Vigilancia em Saude. Belem, PA, BrasilFundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilBACKGROUND: In some tropical countries, such as Brazil, schistosomiasis control programs have led to a significant reduction in the prevalence and parasite burden of endemic populations. In this setting, the Kato-Katz technique, as the standard diagnostic method for the diagnosis of Schistosoma mansoni infections, which involves the analysis of two slides from one fecal sample, loses its sensitivity. As a result, a significant number of infected individuals are not detected. The objective of this study was to perform extensive parasitological testing of up to three fecal samples and include a rapid urine test (POC-CCA) in a moderate prevalence area in Northern Minas Gerais, Brazil, and evaluate the performance of each test separately and in combination. METHODS AND FINDINGS: A total of 254 individuals were examined with variants of the standard Kato-Katz technique (up to18 Kato-Katz slides prepared from three fecal samples), a modified Helmintex (30 g of feces), the saline gradient (500 mg of feces), and the POC-CCA methods. We established a reference standard taking into consideration all the positive results in any of the parasitological exams. Evaluation of the parasite burden by two Kato-Katz slides confirmed that most of the individuals harbored a light infection. When additional slides and different parasitological methods were included, the estimated prevalence rose 2.3 times, from 20.4% to 45.9%. The best sensitivity was obtained with the Helmintex method (84%). All parasitological methods readily detected a high or moderate intensity of infection; however, all lost their high sensitivity in the case of low or very low intensity infections. The overall sensitivity of POC-CCA (64.9%) was similar to the six Kato-Katz slides from three fecal samples. However, POC-CCA showed low concordance (κ = 0.34) when compared with the reference standard. CONCLUSIONS: The recommended Kato-Katz method largely underestimated the prevalence of S. mansoni infection. Because the best performance was achieved with a modified Helmintex method, this technique might serve as a more precise reference standard. An extended number of Kato-Katz slides in combination with other parasitological methods or with POC-CCA was able to detect more than 80% of egg-positive individuals; however, the rapid urine test (POC-CCA) produced a considerable percentage of false positive results.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/28775/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALevaluation methods.pdfevaluation methods.pdfapplication/pdf907517https://www.arca.fiocruz.br/bitstream/icict/28775/2/evaluation%20methods.pdf20d4abbe56d69e0760e17ede6e9e91edMD52TEXTevaluation methods.pdf.txtevaluation methods.pdf.txtExtracted texttext/plain84670https://www.arca.fiocruz.br/bitstream/icict/28775/3/evaluation%20methods.pdf.txt955f30ced60bed4af047e224265fdb95MD53icict/287752019-06-19 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| dc.title.pt_BR.fl_str_mv |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| title |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| spellingShingle |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test Oliveira, Warllem Junio Esquistossomose Schistosomiasis |
| title_short |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| title_full |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| title_fullStr |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| title_full_unstemmed |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| title_sort |
Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test |
| author |
Oliveira, Warllem Junio |
| author_facet |
Oliveira, Warllem Junio Magalhaes, Fernanda do Carmo Elias, Andressa Mariana Saldanha Castro, Vanessa Normandio de Favero, Vivian Lindholz, Catieli Gobetti Oliveira, Aureo Almeida Barbosa, Fernando Sergio Gil, Frederico Gomes, Maria Aparecida Teixeira, Carlos Graeff Enk, Martin Johannes Coelho, Paulo Marcos Zech Carneiro, Mariangela Correa, Deborah Aparecida Negrao Geiger, Stefan Michael |
| author_role |
author |
| author2 |
Magalhaes, Fernanda do Carmo Elias, Andressa Mariana Saldanha Castro, Vanessa Normandio de Favero, Vivian Lindholz, Catieli Gobetti Oliveira, Aureo Almeida Barbosa, Fernando Sergio Gil, Frederico Gomes, Maria Aparecida Teixeira, Carlos Graeff Enk, Martin Johannes Coelho, Paulo Marcos Zech Carneiro, Mariangela Correa, Deborah Aparecida Negrao Geiger, Stefan Michael |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Warllem Junio Magalhaes, Fernanda do Carmo Elias, Andressa Mariana Saldanha Castro, Vanessa Normandio de Favero, Vivian Lindholz, Catieli Gobetti Oliveira, Aureo Almeida Barbosa, Fernando Sergio Gil, Frederico Gomes, Maria Aparecida Teixeira, Carlos Graeff Enk, Martin Johannes Coelho, Paulo Marcos Zech Carneiro, Mariangela Correa, Deborah Aparecida Negrao Geiger, Stefan Michael |
| dc.subject.other.pt_BR.fl_str_mv |
Esquistossomose |
| topic |
Esquistossomose Schistosomiasis |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosomiasis |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil |
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2018 |
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2018-09-13T19:04:22Z |
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2018-09-13T19:04:22Z |
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2018 |
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info:eu-repo/semantics/article |
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OLIVEIRA, Warllem Junio et al. Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test. PLoS Negl Trop Dis., v. 12, n. 2, e0006232, 2018 |
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https://www.arca.fiocruz.br/handle/icict/28775 |
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1935-2735 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0006232 |
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OLIVEIRA, Warllem Junio et al. Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test. PLoS Negl Trop Dis., v. 12, n. 2, e0006232, 2018 1935-2735 10.1371/journal.pntd.0006232 |
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Public Library of Science |
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