Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Maurício Martins
Data de Publicação: 2002
Outros Autores: Soares, Milena Botelho Pereira, Vasconcelos, José Ronnie Carvalho de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9915
Resumo: Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
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spelling Rodrigues, Maurício MartinsSoares, Milena Botelho PereiraVasconcelos, José Ronnie Carvalho de2015-04-08T13:50:04Z2015-04-08T13:50:04Z2002RODRIGUES, M. M.; SOARES, M. B. P.; VASCONCELOS, J. R. C. Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization. Journal of Interferon Cytokine Research, v. 22, n. 11, p. 1137-1141, 2002.1079-9907https://www.arca.fiocruz.br/handle/icict/9915engMary Ann Liebert, IncEndogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilIntramuscular (i.m.) administration of eukaryotic plasmid vectors containing foreign genes is a general immunization strategy capable of inducing protective type 1 immune responses against viral, bacterial, fungal, and parasitic infections. We have described that immunization with a plasmid containing a gene encoding a parasite antigen elicits specific type 1 protective immune responses against experimental infection with the human protozoan parasite Trypanosoma cruzi. However, we had evidence suggesting that DNA immunization concomitantly activated specific type 2 immune responses. To determine precisely the influence of the type 2 cytokine interleukin-4 (IL-4) during DNA immunization, we compared the immune responses of genetically modified IL-4-deficient or wild-type (wt) BALB/c mice. IL-4-deficient mice had a significantly lower ratio of specific serum IgG1/IgG2a, and on in vitro restimulation with antigen, their spleen cells secreted significantly higher amounts of interferon-g (IFN-g). In contrast, absence of IL-4 did not affect total serum antibody response, T cell proliferative responses, or activation of IFN-g-producing CD81 T cells. Our results suggested that in contrast to conventional adjuvants, such as alum and complete Freund’s adjuvant, specific IgG1 in DNA-immunized BALB/c mice was highly dependent on IL-4. To our knowledge, our study provides the first evidence that endogenous IL-4 selectively downregulates the type 1 CD41 T cell-mediated immune response induced by i.m. genetic immunization, a fact that may have implications for the design of certain DNA vaccines.Linfócitos T CD4-Positivos/imunologiaInterleucina-4/imunologiaVacinas de DNA/farmacologiaAnimaisLinfócitos T CD4-Positivos/efeitos de drogasImunidade Celular/efeitos de drogasImunoglobulina G/sangueInjeções IntramuscularesInterleucina-4/deficiênciaCamundongosCamundongos Endogâmicos BALB CCamundongos KnockoutBaço/imunologiaVacinas de DNA/administração & dosageminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9915/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALRodrigues M M Endogenous Interleukin-4....pdfRodrigues M M Endogenous Interleukin-4....pdfapplication/pdf139716https://www.arca.fiocruz.br/bitstream/icict/9915/2/Rodrigues%20M%20M%20Endogenous%20Interleukin-4....pdf8c4e86905945778eab099d02bd8b15dcMD52TEXTRodrigues M M Endogenous Interleukin-4....pdf.txtRodrigues M M Endogenous Interleukin-4....pdf.txtExtracted texttext/plain24159https://www.arca.fiocruz.br/bitstream/icict/9915/3/Rodrigues%20M%20M%20Endogenous%20Interleukin-4....pdf.txtf24addcfaf186048f7f37149e44ea74bMD53icict/99152023-03-15 14:33:21.019oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
title Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
spellingShingle Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
Rodrigues, Maurício Martins
Linfócitos T CD4-Positivos/imunologia
Interleucina-4/imunologia
Vacinas de DNA/farmacologia
Animais
Linfócitos T CD4-Positivos/efeitos de drogas
Imunidade Celular/efeitos de drogas
Imunoglobulina G/sangue
Injeções Intramusculares
Interleucina-4/deficiência
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Baço/imunologia
Vacinas de DNA/administração & dosagem
title_short Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
title_full Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
title_fullStr Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
title_full_unstemmed Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
title_sort Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization.
author Rodrigues, Maurício Martins
author_facet Rodrigues, Maurício Martins
Soares, Milena Botelho Pereira
Vasconcelos, José Ronnie Carvalho de
author_role author
author2 Soares, Milena Botelho Pereira
Vasconcelos, José Ronnie Carvalho de
author2_role author
author
dc.contributor.author.fl_str_mv Rodrigues, Maurício Martins
Soares, Milena Botelho Pereira
Vasconcelos, José Ronnie Carvalho de
dc.subject.decs.pt_BR.fl_str_mv Linfócitos T CD4-Positivos/imunologia
Interleucina-4/imunologia
Vacinas de DNA/farmacologia
Animais
Linfócitos T CD4-Positivos/efeitos de drogas
Imunidade Celular/efeitos de drogas
Imunoglobulina G/sangue
Injeções Intramusculares
Interleucina-4/deficiência
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Baço/imunologia
Vacinas de DNA/administração & dosagem
topic Linfócitos T CD4-Positivos/imunologia
Interleucina-4/imunologia
Vacinas de DNA/farmacologia
Animais
Linfócitos T CD4-Positivos/efeitos de drogas
Imunidade Celular/efeitos de drogas
Imunoglobulina G/sangue
Injeções Intramusculares
Interleucina-4/deficiência
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Baço/imunologia
Vacinas de DNA/administração & dosagem
description Universidade Federal de São Paulo. Escola Paulista de Medicina. 1Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2015-04-08T13:50:04Z
dc.date.available.fl_str_mv 2015-04-08T13:50:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUES, M. M.; SOARES, M. B. P.; VASCONCELOS, J. R. C. Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization. Journal of Interferon Cytokine Research, v. 22, n. 11, p. 1137-1141, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9915
dc.identifier.issn.none.fl_str_mv 1079-9907
identifier_str_mv RODRIGUES, M. M.; SOARES, M. B. P.; VASCONCELOS, J. R. C. Endogenous interleukin-4 downregulates the type 1 CD4 T cell-mediated immune response induced by intramuscular DNA immunization. Journal of Interferon Cytokine Research, v. 22, n. 11, p. 1137-1141, 2002.
1079-9907
url https://www.arca.fiocruz.br/handle/icict/9915
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc
publisher.none.fl_str_mv Mary Ann Liebert, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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