Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20999 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
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Lima, Juliana Figueirêdo da CostaGuedes, Gabriela de Moraes RêgoLima, Juliana Falcão de AraújoLira, Laís Ariane de SiqueiraSantos, Fabiana Cristina FulcoArruda, Mercia Eliane deMontenegro, Lílian Maria LapaSchindler, Haiana Charifker2017-09-13T13:31:01Z2017-09-13T13:31:01Z2015LIMA, J. F. DA C. et al. Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine. Revista Da Sociedade Brasileira De Medicina Tropical, v. 48, n. 6, p. 731–738, dez. 2015.1678-9849https://www.arca.fiocruz.br/handle/icict/2099910.1590/0037-8682-0210-2015porDNA BacterianoMycobacterium tuberculosisReação em Cadeia da PolimeraseTuberculoseAdolescentAdultAgedDNA, BacterialFemaleHumansMaleMiddle AgedMycobacterium tuberculosisgeneticsisolation & purificationPolymerase Chain ReactionTuberculosisReproducibility of ResultsSensitivity and SpecificityYoung AdultAdolescenteAdultoIdosoDNA BacterianoFemininoHumanosMasculinoMeia-IdadeMycobacterium tuberculosisReação em Cadeia da PolimeraseReprodutibilidade dos TestesSensibilidade e EspecificidadeTuberculoseAdulto JovemSingle-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilMolecular analyses are auxiliary tools for detecting Koch's bacilli in clinical specimens from patients with suspected tuberculosis (TB). However, there are still no efficient diagnostic tests that combine high sensitivity and specificity and yield rapid results in the detection of TB. This study evaluated single-tube nested polymerase chain reaction (STNPCR) as a molecular diagnostic test with low risk of cross contamination for detecting Mycobacterium tuberculosis in clinical samples.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20999/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL26676498 2015 lim-sin.oa.pdf26676498 2015 lim-sin.oa.pdfapplication/pdf777675https://www.arca.fiocruz.br/bitstream/icict/20999/2/26676498%202015%20lim-sin.oa.pdfd2897e92d4eb2807c4eba9c6ebccb24dMD52TEXT26676498 2015 lim-sin.oa.pdf.txt26676498 2015 lim-sin.oa.pdf.txtExtracted texttext/plain36458https://www.arca.fiocruz.br/bitstream/icict/20999/3/26676498%202015%20lim-sin.oa.pdf.txtc52a33d8812c6b0aab87136cc2203baeMD53icict/209992018-08-15 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| dc.title.pt_BR.fl_str_mv |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| title |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| spellingShingle |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine Lima, Juliana Figueirêdo da Costa DNA Bacteriano Mycobacterium tuberculosis Reação em Cadeia da Polimerase Tuberculose Adolescent Adult Aged DNA, Bacterial Female Humans Male Middle Aged Mycobacterium tuberculosis genetics isolation & purification Polymerase Chain Reaction Tuberculosis Reproducibility of Results Sensitivity and Specificity Young Adult Adolescente Adulto Idoso DNA Bacteriano Feminino Humanos Masculino Meia-Idade Mycobacterium tuberculosis Reação em Cadeia da Polimerase Reprodutibilidade dos Testes Sensibilidade e Especificidade Tuberculose Adulto Jovem |
| title_short |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| title_full |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| title_fullStr |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| title_full_unstemmed |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| title_sort |
Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine |
| author |
Lima, Juliana Figueirêdo da Costa |
| author_facet |
Lima, Juliana Figueirêdo da Costa Guedes, Gabriela de Moraes Rêgo Lima, Juliana Falcão de Araújo Lira, Laís Ariane de Siqueira Santos, Fabiana Cristina Fulco Arruda, Mercia Eliane de Montenegro, Lílian Maria Lapa Schindler, Haiana Charifker |
| author_role |
author |
| author2 |
Guedes, Gabriela de Moraes Rêgo Lima, Juliana Falcão de Araújo Lira, Laís Ariane de Siqueira Santos, Fabiana Cristina Fulco Arruda, Mercia Eliane de Montenegro, Lílian Maria Lapa Schindler, Haiana Charifker |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Juliana Figueirêdo da Costa Guedes, Gabriela de Moraes Rêgo Lima, Juliana Falcão de Araújo Lira, Laís Ariane de Siqueira Santos, Fabiana Cristina Fulco Arruda, Mercia Eliane de Montenegro, Lílian Maria Lapa Schindler, Haiana Charifker |
| dc.subject.other.pt_BR.fl_str_mv |
DNA Bacteriano Mycobacterium tuberculosis Reação em Cadeia da Polimerase Tuberculose |
| topic |
DNA Bacteriano Mycobacterium tuberculosis Reação em Cadeia da Polimerase Tuberculose Adolescent Adult Aged DNA, Bacterial Female Humans Male Middle Aged Mycobacterium tuberculosis genetics isolation & purification Polymerase Chain Reaction Tuberculosis Reproducibility of Results Sensitivity and Specificity Young Adult Adolescente Adulto Idoso DNA Bacteriano Feminino Humanos Masculino Meia-Idade Mycobacterium tuberculosis Reação em Cadeia da Polimerase Reprodutibilidade dos Testes Sensibilidade e Especificidade Tuberculose Adulto Jovem |
| dc.subject.en.pt_BR.fl_str_mv |
Adolescent Adult Aged DNA, Bacterial Female Humans Male Middle Aged Mycobacterium tuberculosis genetics isolation & purification Polymerase Chain Reaction Tuberculosis Reproducibility of Results Sensitivity and Specificity Young Adult |
| dc.subject.decs.pt_BR.fl_str_mv |
Adolescente Adulto Idoso DNA Bacteriano Feminino Humanos Masculino Meia-Idade Mycobacterium tuberculosis Reação em Cadeia da Polimerase Reprodutibilidade dos Testes Sensibilidade e Especificidade Tuberculose Adulto Jovem |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil |
| publishDate |
2015 |
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2015 |
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2017-09-13T13:31:01Z |
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2017-09-13T13:31:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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LIMA, J. F. DA C. et al. Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine. Revista Da Sociedade Brasileira De Medicina Tropical, v. 48, n. 6, p. 731–738, dez. 2015. |
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https://www.arca.fiocruz.br/handle/icict/20999 |
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1678-9849 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0037-8682-0210-2015 |
| identifier_str_mv |
LIMA, J. F. DA C. et al. Single-tube nested PCR assay with in-house DNA extraction for Mycobacterium tuberculosis detection in blood and urine. Revista Da Sociedade Brasileira De Medicina Tropical, v. 48, n. 6, p. 731–738, dez. 2015. 1678-9849 10.1590/0037-8682-0210-2015 |
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