Field diagnosis and genotyping of chikungunya virus using a dried reverse transcription loop-mediated isothermal amplification (LAMP) assay and MinION sequencing

• Autor(a) principal: Hayashida, Kyoko
• Data de Publicação: 2019
• Outros Autores: Orba, Yasuko, Sequeira, Patricia C., Sugimoto, Chihiro, Hall, William W., Eshita, Yuki, Suzuki, Yutaka, Runtuwene, Lucky, Brasil, Patricia, Calvet, Guilherme, Rodrigues, Cintia D. S., Santos, Carolina C. dos, Mares-Guia, Maria A. M., Yamagishi, Junya, Filippis, Ana M. B. de, Sawa, Hirofumi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
• Texto Completo: https://www.arca.fiocruz.br/handle/icict/61017
• topic: Chikungunya virus (CHIKV); Genome; MinION sequencing; Transcription loop-mediated isothermal amplification (RT-LAMP)

This study was funded by AMED program of the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID; JP18fm0108008) and Research Program on Emerging and Re-emerging Infectious Diseases (JP18fk0108108) and Japan Society for the Promotion of Science (JSPS) KAKENHI (16H05805). This study was also supported by Grants-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (16H06429, 16H06431, 16K21723) and OSIMO foundation. The Flavivirus Laboratory was funded by Faperj (Fundac¸ão Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro) under the grant no. E-26/2002.930/2016, by European Union’s Horizon 2020 program under grant agreements ZIKACTION no. 734857 and ZIKAPLAN no. 734548. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.