Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction

Detalhes bibliográficos
Autor(a) principal: Correa, Alejandro
Data de Publicação: 2018
Outros Autores: Ottoboni, Gabriel Salles, Senegaglia, Alexandra Cristina, Capriglione, Luiz Guilherme Achcar, Miyague, Nelson Itiro, Leite, Lidiane Maria Boldrini, Jamur, Valderez Ravaglio, Rebelatto, Carmen Lúcia Kuniyoshi, Olandoski, Márcia, Brofman, Paulo Roberto Slud
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40109
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Correa, AlejandroOttoboni, Gabriel SallesSenegaglia, Alexandra CristinaCapriglione, Luiz Guilherme AchcarMiyague, Nelson ItiroLeite, Lidiane Maria BoldriniJamur, Valderez RavaglioRebelatto, Carmen Lúcia KuniyoshiOlandoski, MárciaBrofman, Paulo Roberto Slud2020-02-20T18:31:35Z2020-02-20T18:31:35Z2018CORREA, Alejandro et al. Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction. Stem Cells International, p. 1–11, 2018.1687-9678https://www.arca.fiocruz.br/handle/icict/4010910.1155/2018/54124781687-9678porHindawiExpanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarctioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pharmacological approaches are partially effective in limiting infarct size. Cell therapies using a cell population enriched with endothelial progenitor cells (EPCs) CD133+ have opened new perspectives for the treatment of ischemic areas after infarction. This preclinical study evaluated the effect of intramyocardial transplantation of purified or expanded human umbilical cord blood-derived CD133+ cells on the recovery of rats following acute myocardial infarction (AMI). Histology studies, electrocardiogram, and fluorescence in situ hybridization (FISH) were used to evaluate heart recovery. Purified CD133+ cells, enriched in endothelial progenitor cells, when expanded in vitro acquired an endothelial-like cell phenotype expressing CD31 and von Willebrand factor (vWF). The group of infarcted rats that received expanded CD133+ cells had a more significant recovery of contraction performance and less heart remodeling than the group that received purified CD133+ cells. Either purified or expanded CD133+ cells were able to induce neovascularization in the infarcted myocardium in an equivalent manner. Few human cells were detected in the infarcted myocardium of the rats 28 days after transplantation suggesting that the effects observed might be related primarily to paracrine activity. Although both cell populations ameliorated the infarcted heart and are suitable for regeneration of the vascular system, expanded CD133+ cells are more beneficial and promising candidates for vascular regeneration.Endothelial Progenitor CellsMyocardial InfarctionTherapeuticsGuided Tissue RegenerationCélulas Progenitoras EndotelialesInfarto del MiocardioTerapéuticaRegeneración Tisular DirigidaCélulas Progenitoras EndoteliaisInfarto do MiocárdioTerapêuticaRegeneração Tecidual Guiadainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40109/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL5412478ok.pdf5412478ok.pdfapplication/pdf2305810https://www.arca.fiocruz.br/bitstream/icict/40109/2/5412478ok.pdf2150bf8afab087bc945e1e84cba39390MD52TEXT5412478ok.pdf.txt5412478ok.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
title Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
spellingShingle Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
Correa, Alejandro
Endothelial Progenitor Cells
Myocardial Infarction
Therapeutics
Guided Tissue Regeneration
Células Progenitoras Endoteliales
Infarto del Miocardio
Terapéutica
Regeneración Tisular Dirigida
Células Progenitoras Endoteliais
Infarto do Miocárdio
Terapêutica
Regeneração Tecidual Guiada
title_short Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
title_full Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
title_fullStr Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
title_full_unstemmed Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
title_sort Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction
author Correa, Alejandro
author_facet Correa, Alejandro
Ottoboni, Gabriel Salles
Senegaglia, Alexandra Cristina
Capriglione, Luiz Guilherme Achcar
Miyague, Nelson Itiro
Leite, Lidiane Maria Boldrini
Jamur, Valderez Ravaglio
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Márcia
Brofman, Paulo Roberto Slud
author_role author
author2 Ottoboni, Gabriel Salles
Senegaglia, Alexandra Cristina
Capriglione, Luiz Guilherme Achcar
Miyague, Nelson Itiro
Leite, Lidiane Maria Boldrini
Jamur, Valderez Ravaglio
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Márcia
Brofman, Paulo Roberto Slud
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Correa, Alejandro
Ottoboni, Gabriel Salles
Senegaglia, Alexandra Cristina
Capriglione, Luiz Guilherme Achcar
Miyague, Nelson Itiro
Leite, Lidiane Maria Boldrini
Jamur, Valderez Ravaglio
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Márcia
Brofman, Paulo Roberto Slud
dc.subject.en.pt_BR.fl_str_mv Endothelial Progenitor Cells
Myocardial Infarction
Therapeutics
Guided Tissue Regeneration
topic Endothelial Progenitor Cells
Myocardial Infarction
Therapeutics
Guided Tissue Regeneration
Células Progenitoras Endoteliales
Infarto del Miocardio
Terapéutica
Regeneración Tisular Dirigida
Células Progenitoras Endoteliais
Infarto do Miocárdio
Terapêutica
Regeneração Tecidual Guiada
dc.subject.es.pt_BR.fl_str_mv Células Progenitoras Endoteliales
Infarto del Miocardio
Terapéutica
Regeneración Tisular Dirigida
dc.subject.decs.pt_BR.fl_str_mv Células Progenitoras Endoteliais
Infarto do Miocárdio
Terapêutica
Regeneração Tecidual Guiada
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2020-02-20T18:31:35Z
dc.date.available.fl_str_mv 2020-02-20T18:31:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CORREA, Alejandro et al. Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction. Stem Cells International, p. 1–11, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40109
dc.identifier.issn.pt_BR.fl_str_mv 1687-9678
dc.identifier.doi.none.fl_str_mv 10.1155/2018/5412478
dc.identifier.eissn.none.fl_str_mv 1687-9678
identifier_str_mv CORREA, Alejandro et al. Expanded CD133+ Cells from Human Umbilical Cord Blood Improved Heart Function in Rats after Severe Myocardial Infarction. Stem Cells International, p. 1–11, 2018.
1687-9678
10.1155/2018/5412478
url https://www.arca.fiocruz.br/handle/icict/40109
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