Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26332 |
Resumo: | Ministerio de Ciencia e Innovación FISPI14/00366 and FISPI14/00366 (FEDER FUNDING) and the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). CNPq |
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Garde, EstherRamírez, LauraCorvo, LauraSolana, José CMartín, M ElenaGonzález, Víctor MGómez Nieto, CarlosBarral, Aldina Maria PradoBarral Netto, ManoelRequena, José MIborra, SalvadorSoto, Manuel2018-05-08T19:00:15Z2018-05-08T19:00:15Z2018GARDE, E. et al. Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis. Frontiers in Cellular and Infection Microbiology, v.8, abr. 2018.2235-2988https://www.arca.fiocruz.br/handle/icict/2633210.3389/fcimb.2018.00112Ministerio de Ciencia e Innovación FISPI14/00366 and FISPI14/00366 (FEDER FUNDING) and the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). CNPqUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainInstituto Ramón y Cajal de Investigación Sanitaria. Hospital Ramón y Cajal. Departamento de Bioquímica-Investigación. Madrid, SpainInstituto Ramón y Cajal de Investigación Sanitaria. Hospital Ramón y Cajal. Departamento de Bioquímica-Investigación. Madrid, SpainUniversity of Extremadura. Veterinary Faculty. Parasitology Unit. LeishmanCeres Laboratory. Cáceres, SpainFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainCentro Nacional de Investigaciones Cardiovasculares. Department of Vascular Biology and Inflammation. Immunobiology of Inflammation Laboratory. Madrid, Spain / Universidad Complutense de Madrid. School of Medicine. Department of Immunology. Madrid, Spain / Health Research Institute. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias, Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Consejo Superior de Investigaciones Científicas. Madrid, SpainDifferent members of intracellular protein families are recognized by the immune system of the vertebrate host infected by parasites of the genus Leishmania. Here, we have analyzed the antigenic and immunogenic properties of the Leishmania eIF2 and eIF2B translation initiation factors. An in silico search in Leishmania infantum sequence databases allowed the identification of the genes encoding the α, β, and γ subunits and the α, β, and δ subunits of the putative Leishmania orthologs of the eukaryotic initiation factors F2 (LieIF2) or F2B (LieIF2B), respectively. The antigenicity of these factors was analyzed by ELISA using recombinant versions of the different subunits. Antibodies against the different LieIF2 and LieIF2B subunits were found in the sera from human and canine visceral leishmaniasis patients, and also in the sera from hamsters experimentally infected with L. infantum. In L. infantum (BALB/c) and Leishmania major (BALB/c or C57BL/6) challenged mice, a moderate humoral response against these protein factors was detected. Remarkably, these proteins elicited an IL-10 production by splenocytes derived from infected mice independently of the Leishmania species employed for experimental challenge. When DNA vaccines based on the expression of the LieIF2 or LieIF2B subunit encoding genes were administered in mice, an antigen-specific secretion of IFN-γ and IL-10 cytokines was observed. Furthermore, a partial protection against murine CL development due to L. major infection was generated in the vaccinated mice. Also, in this work we show that the LieIF2α subunit and the LieIF2Bβ and δ subunits have the capacity to stimulate IL-10 secretion by spleen cells from naïve mice. B-lymphocytes were identified as the major producers of this anti-inflammatory cytokine. Taking into account the data found in this study, it may be hypothesized that these proteins act as virulence factors implicated in the induction of humoral responses as well as in the production of the down-regulatory IL-10 cytokine, favoring a pathological outcome. Therefore, these proteins might be considered markers of disease.engFrontiers MediaLeishmaniaAantígenosInterleucina-10Leishmaniose visceralFatores de iniciação da traduçãoModelos murinos experimentaisVacinasLeishmaniaAntigensInterleukin-10Visceral leishmaniasisTranslation initiation factorsExperimental murine modelsVaccinesAnalysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26332/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGarde E Analysis of the Antigenic and Prophylactic....pdfGarde E Analysis of the Antigenic and 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| dc.title.pt_BR.fl_str_mv |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| title |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| spellingShingle |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis Garde, Esther Leishmania Aantígenos Interleucina-10 Leishmaniose visceral Fatores de iniciação da tradução Modelos murinos experimentais Vacinas Leishmania Antigens Interleukin-10 Visceral leishmaniasis Translation initiation factors Experimental murine models Vaccines |
| title_short |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| title_full |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| title_fullStr |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| title_full_unstemmed |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| title_sort |
Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis |
| author |
Garde, Esther |
| author_facet |
Garde, Esther Ramírez, Laura Corvo, Laura Solana, José C Martín, M Elena González, Víctor M Gómez Nieto, Carlos Barral, Aldina Maria Prado Barral Netto, Manoel Requena, José M Iborra, Salvador Soto, Manuel |
| author_role |
author |
| author2 |
Ramírez, Laura Corvo, Laura Solana, José C Martín, M Elena González, Víctor M Gómez Nieto, Carlos Barral, Aldina Maria Prado Barral Netto, Manoel Requena, José M Iborra, Salvador Soto, Manuel |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Garde, Esther Ramírez, Laura Corvo, Laura Solana, José C Martín, M Elena González, Víctor M Gómez Nieto, Carlos Barral, Aldina Maria Prado Barral Netto, Manoel Requena, José M Iborra, Salvador Soto, Manuel |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Aantígenos Interleucina-10 Leishmaniose visceral Fatores de iniciação da tradução Modelos murinos experimentais Vacinas |
| topic |
Leishmania Aantígenos Interleucina-10 Leishmaniose visceral Fatores de iniciação da tradução Modelos murinos experimentais Vacinas Leishmania Antigens Interleukin-10 Visceral leishmaniasis Translation initiation factors Experimental murine models Vaccines |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Antigens Interleukin-10 Visceral leishmaniasis Translation initiation factors Experimental murine models Vaccines |
| description |
Ministerio de Ciencia e Innovación FISPI14/00366 and FISPI14/00366 (FEDER FUNDING) and the Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). CNPq |
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2018 |
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2018-05-08T19:00:15Z |
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2018-05-08T19:00:15Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
GARDE, E. et al. Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis. Frontiers in Cellular and Infection Microbiology, v.8, abr. 2018. |
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https://www.arca.fiocruz.br/handle/icict/26332 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fcimb.2018.00112 |
| identifier_str_mv |
GARDE, E. et al. Analysis of the Antigenic and Prophylactic Properties of the Leishmania Translation Initiation Factors eIF2 and eIF2B in Natural and Experimental Leishmaniasis. Frontiers in Cellular and Infection Microbiology, v.8, abr. 2018. 2235-2988 10.3389/fcimb.2018.00112 |
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https://www.arca.fiocruz.br/handle/icict/26332 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Frontiers Media |
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Frontiers Media |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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