Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.

Detalhes bibliográficos
Autor(a) principal: Flannery, Brendan
Data de Publicação: 2001
Outros Autores: Costa, Dirceu Joaquim, Carvalho, Fernanda Pinheiro, Guerreiro, Hygia, Matsunaga, James, Silva, Emilson Domingos da, Ferreira, Antonio Gomes Pinto, Riley, Lee Woodland, Reis, Mitermayer Galvão dos, Haake, David A, Ko, Albert Icksang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7569
Resumo: School of Public Health. University of California. Berkeley, California
id CRUZ_eb5e3cbc46f72d577a4bf8fd82351ffe
oai_identifier_str oai:www.arca.fiocruz.br:icict/7569
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Flannery, BrendanCosta, Dirceu JoaquimCarvalho, Fernanda PinheiroGuerreiro, HygiaMatsunaga, JamesSilva, Emilson Domingos daFerreira, Antonio Gomes PintoRiley, Lee WoodlandReis, Mitermayer Galvão dosHaake, David AKo, Albert Icksang2014-05-02T16:45:58Z2014-05-02T16:45:58Z2001FLANNERY, B. et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. Journal of Clinical Microbiology, v. 39, n. 9, p. 3303-3310, 2001 .0095-1137https://www.arca.fiocruz.br/handle/icict/756910.1128/JCM.39.9.3303–3310.2001engAmerican Society for MicrobiologyEvaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSchool of Public Health. University of California. Berkeley, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / School of Pharmacy. Federal University of Bahia. Salvador, BA, BrasilDivision of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, CaliforniaOswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, BrasilOswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, BrasilSchool of Public Health. University of California. Berkeley, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilDivision of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, CaliforniaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, New YorkThere is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis.Anticorpos Antibacterianos/sangueAntígenos de Bactérias/imunologiaEnsaio de Imunoadsorção Enzimática/métodosLeptospira/imunologiaLeptospirose/diagnósticoAntígenos de Bactérias/genéticaEnsaio de Imunoadsorção Enzimática/normasHumanosImunoglobulina G/sangueLipoproteínas/genéticaLipoproteínas/imunologiaProteínas Recombinantes/genéticaSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFlannery B Evaluation of Recombinant....pdfFlannery B Evaluation of Recombinant....pdfapplication/pdf136164https://www.arca.fiocruz.br/bitstream/icict/7569/1/Flannery%20B%20Evaluation%20of%20Recombinant....pdfce7720255e10d1e840fc729b87625769MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7569/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFlannery B Evaluation of Recombinant....pdf.txtFlannery B Evaluation of Recombinant....pdf.txtExtracted texttext/plain52292https://www.arca.fiocruz.br/bitstream/icict/7569/5/Flannery%20B%20Evaluation%20of%20Recombinant....pdf.txtb0b200be8d3665b67af6eb46d8c8a936MD55THUMBNAILFlannery B Evaluation of Recombinant....pdf.jpgFlannery B Evaluation of Recombinant....pdf.jpgGenerated Thumbnailimage/jpeg2185https://www.arca.fiocruz.br/bitstream/icict/7569/4/Flannery%20B%20Evaluation%20of%20Recombinant....pdf.jpg58f2554d48f90b375a61a2075faaa893MD54icict/75692023-03-15 14:33:02.122oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
title Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
spellingShingle Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
Flannery, Brendan
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
title_short Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
title_full Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
title_fullStr Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
title_full_unstemmed Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
title_sort Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
author Flannery, Brendan
author_facet Flannery, Brendan
Costa, Dirceu Joaquim
Carvalho, Fernanda Pinheiro
Guerreiro, Hygia
Matsunaga, James
Silva, Emilson Domingos da
Ferreira, Antonio Gomes Pinto
Riley, Lee Woodland
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
author_role author
author2 Costa, Dirceu Joaquim
Carvalho, Fernanda Pinheiro
Guerreiro, Hygia
Matsunaga, James
Silva, Emilson Domingos da
Ferreira, Antonio Gomes Pinto
Riley, Lee Woodland
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Flannery, Brendan
Costa, Dirceu Joaquim
Carvalho, Fernanda Pinheiro
Guerreiro, Hygia
Matsunaga, James
Silva, Emilson Domingos da
Ferreira, Antonio Gomes Pinto
Riley, Lee Woodland
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
topic Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
description School of Public Health. University of California. Berkeley, California
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2014-05-02T16:45:58Z
dc.date.available.fl_str_mv 2014-05-02T16:45:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FLANNERY, B. et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. Journal of Clinical Microbiology, v. 39, n. 9, p. 3303-3310, 2001 .
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7569
dc.identifier.issn.none.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.39.9.3303–3310.2001
identifier_str_mv FLANNERY, B. et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. Journal of Clinical Microbiology, v. 39, n. 9, p. 3303-3310, 2001 .
0095-1137
10.1128/JCM.39.9.3303–3310.2001
url https://www.arca.fiocruz.br/handle/icict/7569
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7569/1/Flannery%20B%20Evaluation%20of%20Recombinant....pdf
https://www.arca.fiocruz.br/bitstream/icict/7569/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7569/5/Flannery%20B%20Evaluation%20of%20Recombinant....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7569/4/Flannery%20B%20Evaluation%20of%20Recombinant....pdf.jpg
bitstream.checksum.fl_str_mv ce7720255e10d1e840fc729b87625769
7d48279ffeed55da8dfe2f8e81f3b81f
b0b200be8d3665b67af6eb46d8c8a936
58f2554d48f90b375a61a2075faaa893
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324774193070080

Registros relacionados