Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil

Detalhes bibliográficos
Autor(a) principal: Avelar, Daniel Moreira de
Data de Publicação: 2019
Outros Autores: Carvalho, Débora Moreira, Rabello, Ana Lucia Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39803
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
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spelling Avelar, Daniel Moreira deCarvalho, Débora MoreiraRabello, Ana Lucia Teles2020-02-07T17:11:50Z2020-02-07T17:11:50Z2019AVELAR, Daniel Moreira de; CARVALHO, Débora Moreira; RABELLO, Ana Lucia Teles. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International, v. 1, p. 1-7, 2019.2314-6141https://www.arca.fiocruz.br/handle/icict/3980310.1155/2019/8240784engHindawi Pub. CoDevelopment and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.Visceral leishmaniasis (VL) is considered a major public health concern in Brazil and several regions of the world. A recent advance in the diagnosis of infectious diseases was the development of loop-mediated isothermal amplification (LAMP). The aim of this study was to develop and evaluate a new LAMP assay for detection of K26 antigen-coding gene of L. donovani complex. A total of 219 blood samples of immunocompetent patients, including 114 VL cases and 105 non-VL cases, were analyzed for the diagnosis of VL in the present study. Diagnostic accuracy was calculated against a combination of parasitological and/or serological tests as a reference standard. The results were compared to those of kDNA Leishmania-PCR. The detection limit for the K26-Lamp assay was 1fg L. infantum purified DNA and 100 parasites/mL within 60 min of amplification time with visual detection for turbidity. The assay was specific for L. donovani complex. Sensitivity, specificity, and accuracy were 98.2%, 98.1%, and 98.2%, respectively, for K26-LAMP and 100%, 100%, and 100%, respectively, for kDNA Leishmania-PCR. Excellent agreement was observed between K26-LAMP and kDNA Leishmania-PCR assays (K = 0.96). A highly sensitive and specific LAMP assay targeting K26 antigen-coding gene of L. donovani complex was developed for diagnosis in peripheral blood samples of VL patients.Visceral leishmaniasisL. donovaniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/39803/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDevelopment_and_Clinical_Evaluation_of_Loop-Mediat.pdfDevelopment_and_Clinical_Evaluation_of_Loop-Mediat.pdfapplication/pdf2250805https://www.arca.fiocruz.br/bitstream/icict/39803/2/Development_and_Clinical_Evaluation_of_Loop-Mediat.pdfce0d44382fff6ea78478cd40f2f41843MD52TEXTDevelopment_and_Clinical_Evaluation_of_Loop-Mediat.pdf.txtDevelopment_and_Clinical_Evaluation_of_Loop-Mediat.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
title Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
spellingShingle Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
Avelar, Daniel Moreira de
Visceral leishmaniasis
L. donovani
title_short Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
title_full Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
title_fullStr Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
title_full_unstemmed Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
title_sort Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
author Avelar, Daniel Moreira de
author_facet Avelar, Daniel Moreira de
Carvalho, Débora Moreira
Rabello, Ana Lucia Teles
author_role author
author2 Carvalho, Débora Moreira
Rabello, Ana Lucia Teles
author2_role author
author
dc.contributor.author.fl_str_mv Avelar, Daniel Moreira de
Carvalho, Débora Moreira
Rabello, Ana Lucia Teles
dc.subject.en.pt_BR.fl_str_mv Visceral leishmaniasis
L. donovani
topic Visceral leishmaniasis
L. donovani
description Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-02-07T17:11:50Z
dc.date.available.fl_str_mv 2020-02-07T17:11:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AVELAR, Daniel Moreira de; CARVALHO, Débora Moreira; RABELLO, Ana Lucia Teles. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International, v. 1, p. 1-7, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39803
dc.identifier.issn.pt_BR.fl_str_mv 2314-6141
dc.identifier.doi.none.fl_str_mv 10.1155/2019/8240784
identifier_str_mv AVELAR, Daniel Moreira de; CARVALHO, Débora Moreira; RABELLO, Ana Lucia Teles. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International, v. 1, p. 1-7, 2019.
2314-6141
10.1155/2019/8240784
url https://www.arca.fiocruz.br/handle/icict/39803
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Pub. Co
publisher.none.fl_str_mv Hindawi Pub. Co
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/39803/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/39803/2/Development_and_Clinical_Evaluation_of_Loop-Mediat.pdf
https://www.arca.fiocruz.br/bitstream/icict/39803/3/Development_and_Clinical_Evaluation_of_Loop-Mediat.pdf.txt
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