1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19662 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil. |
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Granato, Marcela QueirozGonçalves, Diego de SouzaSeabra, Sergio HenriqueMcCann, MalachyDevereux, MichaelSantos, André Luis Souza dosKneipp, Lucimar Ferreira2017-06-29T12:36:59Z2017-06-29T12:36:59Z2017GRANATO, Marcela Queiroz; et al. 1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa. Front Microbiol., v.8, Article 76, 9p, Jan. 2017.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/1966210.3389/fmicb.2017.00076engFrontiers MediaCromoblastomicoseAtividade antifúngicaDrogas à base de metalPhialophora verrucosa1,10-fenantrolina-5,6-dionaPhialophora verrucosa1,10-phenanthroline-5,6-dionemetal-based drugsantifungal activitychromoblastomycosis1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil / Universidade Federal do Ri de Janeiro. Instituto de Química. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Cultura de Células. Duque de Caxias, RJ, Brasil.National University of Ireland. Maynooth Universit. Chemistry Department. Maynooth, Ireland.Dublin Institute of Technolog. , Focas Research Institute. Centre for Biomimetic and Therapeutic Research. Dublin, Ireland.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases. Rio de Janeiro, RJ, Brasil / Universidade Federal do Ri de Janeiro. Instituto de Química. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Phialophora verrucosa is a dematiaceous fungus able to cause chromoblastomycosis, phaeohyphomycosis and mycetoma. All these fungal diseases are extremely difficult to treat and often refractory to the current therapeutic approaches. Therefore, there is an urgent necessity to develop new antifungal agents to combat these mycoses. In this context, the aim of the present work was to investigate the effect of 1,10-phenanthroline-5,6-dione (phendione) and its metal-based derivatives [Ag(phendione)2]ClO4 = ([Ag(phendione)2](+)) and [Cu(phendione)3](ClO4)2.4H2O = ([Cu(phendione)3](2+)) on crucial physiological events of P. verrucosa conidial cells. Using the CLSI protocol, we have shown that phendione, [Ag(phendione)2](+) and [Cu(phendione)3](2+) were able to inhibit fungal proliferation, presenting MIC/IC50 values of 12.0/7.0, 4.0/2.4, and 5.0/1.8 μM, respectively. [Cu(phendione)3](2+) had fungicidal action and when combined with amphotericin B, both at sub-MIC (½ × MIC) concentrations, significantly reduced (~40%) the fungal growth. Cell morphology changes inflicted by phendione and its metal-based derivatives was corroborated by scanning electron microscopy, which revealed irreversible ultrastructural changes like surface invaginations, cell disruption and shrinkages. Furthermore, [Cu(phendione)3](2+) and [Ag(phendione)2](+) were able to inhibit metallopeptidase activity secreted by P. verrucosa conidia by approximately 85 and 40%, respectively. Ergosterol content was reduced (~50%) after the treatment of P. verrucosa conidial cells with both phendione and [Ag(phendione)2](+). To different degrees, all of the test compounds were able to disturb the P. verrucosa conidia-into-mycelia transformation. Phendione and its Ag(+) and Cu(2+) complexes may represent a promising new group of antimicrobial agents effective at inhibiting P. verrucosa growth and morphogenesis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19662/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarcela_granato_etal_IOC_2017.pdfmarcela_granato_etal_IOC_2017.pdfapplication/pdf2241233https://www.arca.fiocruz.br/bitstream/icict/19662/2/marcela_granato_etal_IOC_2017.pdfa1cd1bf248de0fc64aad02ebd88c1f03MD52TEXTmarcela_granato_etal_IOC_2017.pdf.txtmarcela_granato_etal_IOC_2017.pdf.txtExtracted texttext/plain46646https://www.arca.fiocruz.br/bitstream/icict/19662/3/marcela_granato_etal_IOC_2017.pdf.txt42e7f61bee08fbad1a9d64d2498ee5afMD53icict/196622018-08-15 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| dc.title.pt_BR.fl_str_mv |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| title |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| spellingShingle |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa Granato, Marcela Queiroz Cromoblastomicose Atividade antifúngica Drogas à base de metal Phialophora verrucosa 1,10-fenantrolina-5,6-diona Phialophora verrucosa 1,10-phenanthroline-5,6-dione metal-based drugs antifungal activity chromoblastomycosis |
| title_short |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| title_full |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| title_fullStr |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| title_full_unstemmed |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| title_sort |
1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa |
| author |
Granato, Marcela Queiroz |
| author_facet |
Granato, Marcela Queiroz Gonçalves, Diego de Souza Seabra, Sergio Henrique McCann, Malachy Devereux, Michael Santos, André Luis Souza dos Kneipp, Lucimar Ferreira |
| author_role |
author |
| author2 |
Gonçalves, Diego de Souza Seabra, Sergio Henrique McCann, Malachy Devereux, Michael Santos, André Luis Souza dos Kneipp, Lucimar Ferreira |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Granato, Marcela Queiroz Gonçalves, Diego de Souza Seabra, Sergio Henrique McCann, Malachy Devereux, Michael Santos, André Luis Souza dos Kneipp, Lucimar Ferreira |
| dc.subject.other.pt_BR.fl_str_mv |
Cromoblastomicose Atividade antifúngica Drogas à base de metal Phialophora verrucosa 1,10-fenantrolina-5,6-diona |
| topic |
Cromoblastomicose Atividade antifúngica Drogas à base de metal Phialophora verrucosa 1,10-fenantrolina-5,6-diona Phialophora verrucosa 1,10-phenanthroline-5,6-dione metal-based drugs antifungal activity chromoblastomycosis |
| dc.subject.en.pt_BR.fl_str_mv |
Phialophora verrucosa 1,10-phenanthroline-5,6-dione metal-based drugs antifungal activity chromoblastomycosis |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil. |
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2017 |
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2017-06-29T12:36:59Z |
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2017-06-29T12:36:59Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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GRANATO, Marcela Queiroz; et al. 1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa. Front Microbiol., v.8, Article 76, 9p, Jan. 2017. |
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https://www.arca.fiocruz.br/handle/icict/19662 |
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1664-302X |
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10.3389/fmicb.2017.00076 |
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GRANATO, Marcela Queiroz; et al. 1,10-Phenanthroline-5,6-Dione-Based Compounds Are Effective in Disturbing Crucial Physiological Events of Phialophora verrucosa. Front Microbiol., v.8, Article 76, 9p, Jan. 2017. 1664-302X 10.3389/fmicb.2017.00076 |
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