Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil

Bibliographic Details
Main Author: Silva, Tatiane
Publication Date: 2008
Other Authors: Nogueira, Paulo Afonso, Magalhães, Gleiciene Félix, Grava, Andréa Fagundes, Silva, Luiz Hildebrando Pereira da, Orlandi, Patricia Puccinelli
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/1933
Summary: Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
id CRUZ_f609a49b7a5908c10ae92ea464ba3158
oai_identifier_str oai:www.arca.fiocruz.br:icict/1933
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Silva, TatianeNogueira, Paulo AfonsoMagalhães, Gleiciene FélixGrava, Andréa FagundesSilva, Luiz Hildebrando Pereira daOrlandi, Patricia Puccinelli2011-04-22T18:07:15Z2011-04-22T18:07:15Z2008SILVA, Tatiane et al. Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 731-733, Nov. 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/1933engCharacterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Leônidas e Maria Deane. Manaus. AM, Brasil.The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.Shigella sppBrazilMultiple antibiotic resistanceFiarrheaIpa genesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCharacterization of Shigella spp.pdfCharacterization of Shigella spp.pdfapplication/pdf289083https://www.arca.fiocruz.br/bitstream/icict/1933/1/Characterization%20of%20Shigella%20spp.pdf97ca99184537e2189c34ad0371098209MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/1933/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTCharacterization of Shigella spp.pdf.txtCharacterization of Shigella spp.pdf.txtExtracted texttext/plain12405https://www.arca.fiocruz.br/bitstream/icict/1933/5/Characterization%20of%20Shigella%20spp.pdf.txt0c5f52d3e537aa9b38949a213050a303MD55THUMBNAILCharacterization of Shigella spp.pdf.jpgCharacterization of Shigella spp.pdf.jpgGenerated Thumbnailimage/jpeg2382https://www.arca.fiocruz.br/bitstream/icict/1933/4/Characterization%20of%20Shigella%20spp.pdf.jpg302ea396635671b83e7760a0fe40121aMD54icict/19332018-04-06 09:14:36.979oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:14:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
title Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
spellingShingle Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Silva, Tatiane
Shigella spp
Brazil
Multiple antibiotic resistance
Fiarrhea
Ipa genes
title_short Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
title_full Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
title_fullStr Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
title_full_unstemmed Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
title_sort Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
author Silva, Tatiane
author_facet Silva, Tatiane
Nogueira, Paulo Afonso
Magalhães, Gleiciene Félix
Grava, Andréa Fagundes
Silva, Luiz Hildebrando Pereira da
Orlandi, Patricia Puccinelli
author_role author
author2 Nogueira, Paulo Afonso
Magalhães, Gleiciene Félix
Grava, Andréa Fagundes
Silva, Luiz Hildebrando Pereira da
Orlandi, Patricia Puccinelli
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Tatiane
Nogueira, Paulo Afonso
Magalhães, Gleiciene Félix
Grava, Andréa Fagundes
Silva, Luiz Hildebrando Pereira da
Orlandi, Patricia Puccinelli
dc.subject.en.pt_BR.fl_str_mv Shigella spp
Brazil
Multiple antibiotic resistance
Fiarrhea
Ipa genes
topic Shigella spp
Brazil
Multiple antibiotic resistance
Fiarrhea
Ipa genes
description Centro de Pesquisa em Medicina Tropical. Porto Velho, RO, Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2011-04-22T18:07:15Z
dc.date.available.fl_str_mv 2011-04-22T18:07:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Tatiane et al. Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 731-733, Nov. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/1933
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv SILVA, Tatiane et al. Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil. Memória do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 7, p. 731-733, Nov. 2008.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/1933
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/1933/1/Characterization%20of%20Shigella%20spp.pdf
https://www.arca.fiocruz.br/bitstream/icict/1933/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/1933/5/Characterization%20of%20Shigella%20spp.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/1933/4/Characterization%20of%20Shigella%20spp.pdf.jpg
bitstream.checksum.fl_str_mv 97ca99184537e2189c34ad0371098209
e095249ac7cacefbfe39684dfe45e706
0c5f52d3e537aa9b38949a213050a303
302ea396635671b83e7760a0fe40121a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324933866029056

Similar Items