Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.

Detalhes bibliográficos
Autor(a) principal: Pereira, Roberta Verciano
Data de Publicação: 2014
Outros Autores: Vieira, Helaine Graziele Santos, Oliveira, Victor Fernandes de, Gomes, Matheus de Souza, Passos, Liana Konovaloff Jannotti, Borges, William de Castro, Sá, Renata Guerra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9432
Resumo: Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brasil
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spelling Pereira, Roberta VercianoVieira, Helaine Graziele SantosOliveira, Victor Fernandes deGomes, Matheus de SouzaPassos, Liana Konovaloff JannottiBorges, William de CastroSá, Renata Guerra2015-02-04T17:13:45Z2015-02-04T17:13:45Z2014PEREIRA, Roberta Verciano et al. Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni. Mem Inst Oswaldo Cruz, v. 109, n. 1, p. 1-8, 2014.0074-0276https://www.arca.fiocruz.br/handle/icict/943210.1590/0074-0276130107engFundação Oswaldo CruzConservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Patos de Minas, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilSeveral genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and shown to play an important role in Ub-dependent processes, little is known about their role in S. mansoni. In this study, we analysed the Ub carboxyl-terminal hydrolase (UCHs) proteins found in the database of the parasite’s genome. An in silico analysis (GeneDB and MEROPS) identified three different UCH family members in the genome, SmUCH-L3, SmUCHL5 and SmBAP-1 and a phylogenetic analysis confirmed the evolutionary conservation of the proteins. We performed quantitative reverse transcription-polymerase chain reaction and observed a differential expression profile for all of the investigated transcripts between the cercariae and adult worm stages. These results were corroborated by low rates of Z-Arg-Leu-Arg-Gly-Gly-AMC hydrolysis in a crude extract obtained from cercariae in parallel with high Ub conjugate levels in the same extracts. We suggest that the accumulation of ubiquitinated proteins in the cercaria and early schistosomulum stages is related to a decrease in 26S proteasome activity. Taken together, our data suggest that UCH family members contribute to regulating the activity of the Ub-proteasome system during the life cycle of this parasite.deubiquitinating enzymesubiquitin C-terminal hydrolasedifferential expressiondeubiquitination activityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9432/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_038.pdf2014_038.pdfapplication/pdf960239https://www.arca.fiocruz.br/bitstream/icict/9432/2/2014_038.pdfc66206da0fb67290ca28f0d07e86b538MD52TEXT2014_038.pdf.txt2014_038.pdf.txtExtracted texttext/plain38584https://www.arca.fiocruz.br/bitstream/icict/9432/3/2014_038.pdf.txtb4dbd74b6cf95d5cbc587dce589d081aMD53icict/94322019-06-19 10:07:38.237oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
title Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
spellingShingle Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
Pereira, Roberta Verciano
deubiquitinating enzymes
ubiquitin C-terminal hydrolase
differential expression
deubiquitination activity
title_short Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
title_full Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
title_fullStr Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
title_full_unstemmed Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
title_sort Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.
author Pereira, Roberta Verciano
author_facet Pereira, Roberta Verciano
Vieira, Helaine Graziele Santos
Oliveira, Victor Fernandes de
Gomes, Matheus de Souza
Passos, Liana Konovaloff Jannotti
Borges, William de Castro
Sá, Renata Guerra
author_role author
author2 Vieira, Helaine Graziele Santos
Oliveira, Victor Fernandes de
Gomes, Matheus de Souza
Passos, Liana Konovaloff Jannotti
Borges, William de Castro
Sá, Renata Guerra
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Roberta Verciano
Vieira, Helaine Graziele Santos
Oliveira, Victor Fernandes de
Gomes, Matheus de Souza
Passos, Liana Konovaloff Jannotti
Borges, William de Castro
Sá, Renata Guerra
dc.subject.en.pt_BR.fl_str_mv deubiquitinating enzymes
ubiquitin C-terminal hydrolase
differential expression
deubiquitination activity
topic deubiquitinating enzymes
ubiquitin C-terminal hydrolase
differential expression
deubiquitination activity
description Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, Brasil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-04T17:13:45Z
dc.date.available.fl_str_mv 2015-02-04T17:13:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Roberta Verciano et al. Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni. Mem Inst Oswaldo Cruz, v. 109, n. 1, p. 1-8, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9432
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276130107
identifier_str_mv PEREIRA, Roberta Verciano et al. Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni. Mem Inst Oswaldo Cruz, v. 109, n. 1, p. 1-8, 2014.
0074-0276
10.1590/0074-0276130107
url https://www.arca.fiocruz.br/handle/icict/9432
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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