Insights into the transcriptome of oenocytes from Aedes aegypti pupae.

Detalhes bibliográficos
Autor(a) principal: Martins, Gustavo Ferreira
Data de Publicação: 2011
Outros Autores: Ortigão, José Marcelo Ramalho, Lobo, Neil Francis, Severson, David William, McDowel, Mary Ann, Pimenta, Paulo Filemon Paolucci
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8936
Resumo: Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, Brasil
id CRUZ_f85e7c5d5c0f183e2a95da2aab8a61ed
oai_identifier_str oai:www.arca.fiocruz.br:icict/8936
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Martins, Gustavo FerreiraOrtigão, José Marcelo RamalhoLobo, Neil FrancisSeverson, David WilliamMcDowel, Mary AnnPimenta, Paulo Filemon Paolucci2014-11-21T14:36:12Z2014-11-21T14:36:12Z2011MARTINS, Gustavo Ferreira et al. Insights into the transcriptome of oenocytes from Aedes aegypti pupae. Memorias do Instituto Oswaldo Cruz, v. 106, n. 3 p. 308-315, 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/893610.1590/S0074-02762011000300009engFundação Oswaldo Cruz.Insights into the transcriptome of oenocytes from Aedes aegypti pupae.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, BrasilKansas State University. Department of Entomology. Manhattan, Kansas, USAUniversity of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USAUniversity of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USAUniversity of Notre Dame. Center for Global Health and Infectious Diseases. Department of Biological Sciences. Notre Dame, IN, USAFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrasilOenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. Although oenocytes were identified over a century ago, little is known about their messenger RNA expression profiles. In this study, we investigated the transcriptome of Aedes aegypti oenocytes. We constructed a cDNA library from Ae. aegypti MOYO-R strain oenocytes collected from pupae and randomly sequenced 687 clones. After sequences editing and assembly, 326 high-quality contigs were generated. The most abundant transcripts identified corresponded to the cytochrome P450 superfamily, whose members have roles primarily related to detoxification and lipid metabolism. In addition, we identified 18 other transcripts with putative functions associated with lipid metabolism. One such transcript, a fatty acid synthase, is highly represented in the cDNA library of oenocytes. Moreover, oenocytes expressed several immunity-related genes and the majority of these genes were lysozymes. The transcriptional profile suggests that oenocytes play diverse roles, such as detoxification and lipid metabolism, and increase our understanding of the importance of oenocytes in Ae. aegypti homeostasis and immune competenceAedes aegyptioenocytestranscriptomecytochrome P450lipid metabolismdetoxificationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8936/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALInsights into the transcriptome of oenocytes from Aedes aegypti pupae.pdfInsights into the transcriptome of oenocytes from Aedes aegypti pupae.pdfapplication/pdf473207https://www.arca.fiocruz.br/bitstream/icict/8936/2/Insights%20into%20the%20transcriptome%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdfa8c9cdeeabe1e3f508b3bd6da2617f1bMD52TEXTInsights into the transcriptome of oenocytes from Aedes aegypti pupae.pdf.txtInsights into the transcriptome of oenocytes from Aedes aegypti pupae.pdf.txtExtracted texttext/plain41366https://www.arca.fiocruz.br/bitstream/icict/8936/3/Insights%20into%20the%20transcriptome%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdf.txta594e44b4e66188f869a118a3c200569MD53icict/89362019-06-19 10:11:44.411oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:11:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
title Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
spellingShingle Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
Martins, Gustavo Ferreira
Aedes aegypti
oenocytes
transcriptome
cytochrome P450
lipid metabolism
detoxification
title_short Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
title_full Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
title_fullStr Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
title_full_unstemmed Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
title_sort Insights into the transcriptome of oenocytes from Aedes aegypti pupae.
author Martins, Gustavo Ferreira
author_facet Martins, Gustavo Ferreira
Ortigão, José Marcelo Ramalho
Lobo, Neil Francis
Severson, David William
McDowel, Mary Ann
Pimenta, Paulo Filemon Paolucci
author_role author
author2 Ortigão, José Marcelo Ramalho
Lobo, Neil Francis
Severson, David William
McDowel, Mary Ann
Pimenta, Paulo Filemon Paolucci
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Gustavo Ferreira
Ortigão, José Marcelo Ramalho
Lobo, Neil Francis
Severson, David William
McDowel, Mary Ann
Pimenta, Paulo Filemon Paolucci
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
oenocytes
transcriptome
cytochrome P450
lipid metabolism
detoxification
topic Aedes aegypti
oenocytes
transcriptome
cytochrome P450
lipid metabolism
detoxification
description Universidade Federal de Viçosa. Departamento de Biologia Geral. Viçosa, MG, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-21T14:36:12Z
dc.date.available.fl_str_mv 2014-11-21T14:36:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Gustavo Ferreira et al. Insights into the transcriptome of oenocytes from Aedes aegypti pupae. Memorias do Instituto Oswaldo Cruz, v. 106, n. 3 p. 308-315, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8936
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762011000300009
identifier_str_mv MARTINS, Gustavo Ferreira et al. Insights into the transcriptome of oenocytes from Aedes aegypti pupae. Memorias do Instituto Oswaldo Cruz, v. 106, n. 3 p. 308-315, 2011.
0074-0276
10.1590/S0074-02762011000300009
url https://www.arca.fiocruz.br/handle/icict/8936
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8936/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/8936/2/Insights%20into%20the%20transcriptome%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdf
https://www.arca.fiocruz.br/bitstream/icict/8936/3/Insights%20into%20the%20transcriptome%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
a8c9cdeeabe1e3f508b3bd6da2617f1b
a594e44b4e66188f869a118a3c200569
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324721238933504

Registros relacionados