Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis

Detalhes bibliográficos
Autor(a) principal: Cordeiro, Taps Aparecida Reis
Data de Publicação: 2020
Outros Autores: Martins, Helen Rodrigues, Franco, Diego Leoni, Santos, Fred Luciano Neves, Celedon, Paola Alejandra Fiorani, Cantuaria, Vinicius Lopes, Lana, Marta de, Reis, Alexandre Barbosa, Ferreira, Lucas Franco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44242
Resumo: Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15).
id CRUZ_fa9d68680c152c37dba381ca21dc3ffb
oai_identifier_str oai:www.arca.fiocruz.br:icict/44242
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Cordeiro, Taps Aparecida ReisMartins, Helen RodriguesFranco, Diego LeoniSantos, Fred Luciano NevesCeledon, Paola Alejandra FioraniCantuaria, Vinicius LopesLana, Marta deReis, Alexandre BarbosaFerreira, Lucas Franco2020-10-28T12:53:03Z2020-10-28T12:53:03Z2020CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020.0956-5663https://www.arca.fiocruz.br/handle/icict/4424210.1016/j.bios.2020.112573Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15).In this work, a dual detection system based on an impedimetric immunosensor was developed for the lust time for the simultaneous detection of anti-Tgpanosoma cruzi and anti-Leishmania infanturn antibodies in human and dog serum samples. The IBMP 8.1 and rLcilA/rLci2B recombinant antigens were inunobilized over the surface of dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a dilution of 1:10,240 and for anti-L. infant-tun up to 1:5120 in canine seams samples. Relative standard deviation (RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L infannun antigen (W2) samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 °C for 8 weeks, with activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as well as no cross-reactivity could be detected among sera with other infections. With this approach, immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter Phase-II studies.engElsevierChagasLeishmaniose visceralDetecção duplaImunossensor impedimétricoEletropolimerizaçãoChagasVisceral leishmaniasisDual detectionImpedimetric immunosensorElectropolymerizationImpedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/44242/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALCordeiro, T.A.R. Impedimetric immunosensor....pdfCordeiro, T.A.R. Impedimetric immunosensor....pdfapplication/pdf5200482https://www.arca.fiocruz.br/bitstream/icict/44242/2/Cordeiro%2c%20T.A.R.%20Impedimetric%20immunosensor....pdf0fa82fb7681d38814e282fca47cc5c80MD52TEXTCordeiro, T.A.R. Impedimetric immunosensor....pdf.txtCordeiro, T.A.R. Impedimetric immunosensor....pdf.txtExtracted texttext/plain8https://www.arca.fiocruz.br/bitstream/icict/44242/3/Cordeiro%2c%20T.A.R.%20Impedimetric%20immunosensor....pdf.txt8d1b69dd9bdc9df4a8073c7a8193c7afMD53icict/442422023-03-15 14:34:23.102oai:www.arca.fiocruz.br:icict/44242Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUw0KDQpBbmEgTWFyaWEgRmlzY2luYSBTYW1wYWlvLCBDUEY6IDA2NS40MzEuMDM1LTE1LCB2aW5jdWxhZG8gYSBDUHFHTSAtIENlbnRybyBkZSBQZXNxdWlzYXMgR29uw6dhbG8gTW9uaXoKCkFvIGFjZWl0YXIgb3MgVEVSTU9TIGUgQ09OREnDh8OVRVMgZGVzdGEgQ0VTU8ODTywgbyBBVVRPUiBlL291IFRJVFVMQVIgZGUgZGlyZWl0b3MKYXV0b3JhaXMgc29icmUgYSBPQlJBIGRlIHF1ZSB0cmF0YSBlc3RlIGRvY3VtZW50bzoKCigxKSBDRURFIGUgVFJBTlNGRVJFLCB0b3RhbCBlIGdyYXR1aXRhbWVudGUsIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiwgZW0KY2Fyw6F0ZXIgcGVybWFuZW50ZSwgaXJyZXZvZ8OhdmVsIGUgTsODTyBFWENMVVNJVk8sIHRvZG9zIG9zIGRpcmVpdG9zIHBhdHJpbW9uaWFpcyBOw4NPCkNPTUVSQ0lBSVMgZGUgdXRpbGl6YcOnw6NvIGRhIE9CUkEgYXJ0w61zdGljYSBlL291IGNpZW50w61maWNhIGluZGljYWRhIGFjaW1hLCBpbmNsdXNpdmUgb3MgZGlyZWl0b3MKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0KcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKCigyKSBBQ0VJVEEgcXVlIGEgY2Vzc8OjbyB0b3RhbCBuw6NvIGV4Y2x1c2l2YSwgcGVybWFuZW50ZSBlIGlycmV2b2fDoXZlbCBkb3MgZGlyZWl0b3MgYXV0b3JhaXMKcGF0cmltb25pYWlzIG7Do28gY29tZXJjaWFpcyBkZSB1dGlsaXphw6fDo28gZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvIGluY2x1aSwgZXhlbXBsaWZpY2F0aXZhbWVudGUsCm9zIGRpcmVpdG9zIGRlIGRpc3BvbmliaWxpemHDp8OjbyBlIGNvbXVuaWNhw6fDo28gcMO6YmxpY2EgZGEgT0JSQSwgZW0gcXVhbHF1ZXIgbWVpbyBvdSB2ZcOtY3VsbywKaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywKZGVjbGFtYcOnw6NvLCByZWNpdGHDp8OjbywgZXhwb3Npw6fDo28sIGFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywKZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGR1YmxhZ2VtLCBsZWdlbmRhZ2VtLCBpbmNsdXPDo28gZW0gbm92YXMgb2JyYXMgb3UKY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUKdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwoKKDMpIFJFQ09OSEVDRSBxdWUgYSBjZXNzw6NvIGFxdWkgZXNwZWNpZmljYWRhIGNvbmNlZGUgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETwpDUlVaIG8gZGlyZWl0byBkZSBhdXRvcml6YXIgcXVhbHF1ZXIgcGVzc29hIOKAkyBmw61zaWNhIG91IGp1csOtZGljYSwgcMO6YmxpY2Egb3UgcHJpdmFkYSwgbmFjaW9uYWwgb3UKZXN0cmFuZ2VpcmEg4oCTIGEgYWNlc3NhciBlIHV0aWxpemFyIGFtcGxhbWVudGUgYSBPQlJBLCBzZW0gZXhjbHVzaXZpZGFkZSwgcGFyYSBxdWFpc3F1ZXIKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwoKKDQpIERFQ0xBUkEgcXVlIGEgb2JyYSDDqSBjcmlhw6fDo28gb3JpZ2luYWwgZSBxdWUgw6kgbyB0aXR1bGFyIGRvcyBkaXJlaXRvcyBhcXVpIGNlZGlkb3MgZSBhdXRvcml6YWRvcywKcmVzcG9uc2FiaWxpemFuZG8tc2UgaW50ZWdyYWxtZW50ZSBwZWxvIGNvbnRlw7pkbyBlIG91dHJvcyBlbGVtZW50b3MgcXVlIGZhemVtIHBhcnRlIGRhIE9CUkEsCmluY2x1c2l2ZSBvcyBkaXJlaXRvcyBkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBvYnJpZ2FuZG8tc2UgYSBpbmRlbml6YXIgdGVyY2Vpcm9zIHBvcgpkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIGRlCmV2ZW50dWFpcyBkZXNwZXNhcyBxdWUgdmllcmVtIGEgc3Vwb3J0YXIsIGVtIHJhesOjbyBkZSBxdWFscXVlciBvZmVuc2EgYSBkaXJlaXRvcyBhdXRvcmFpcyBvdQpkaXJlaXRvcyBkZSB2b3ogb3UgaW1hZ2VtLCBwcmluY2lwYWxtZW50ZSBubyBxdWUgZGl6IHJlc3BlaXRvIGEgcGzDoWdpbyBlIHZpb2xhw6fDtWVzIGRlIGRpcmVpdG9zOwoKKDUpIEFGSVJNQSBxdWUgY29uaGVjZSBhIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08KT1NXQUxETyBDUlVaIGUgYXMgZGlyZXRyaXplcyBwYXJhIG8gZnVuY2lvbmFtZW50byBkbyByZXBvc2l0w7NyaW8gaW5zdGl0dWNpb25hbCBBUkNBLgoKQSBQb2zDrXRpY2EgSW5zdGl0dWNpb25hbCBkZSBBY2Vzc28gQWJlcnRvIGRhIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiByZXNlcnZhCmV4Y2x1c2l2YW1lbnRlIGFvIEFVVE9SIG9zIGRpcmVpdG9zIG1vcmFpcyBlIG9zIHVzb3MgY29tZXJjaWFpcyBzb2JyZSBhcyBvYnJhcyBkZSBzdWEgYXV0b3JpYQplL291IHRpdHVsYXJpZGFkZSwgc2VuZG8gb3MgdGVyY2Vpcm9zIHVzdcOhcmlvcyByZXNwb25zw6F2ZWlzIHBlbGEgYXRyaWJ1acOnw6NvIGRlIGF1dG9yaWEgZSBtYW51dGVuw6fDo28KZGEgaW50ZWdyaWRhZGUgZGEgT0JSQSBlbSBxdWFscXVlciB1dGlsaXphw6fDo28uCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaCnJlc3BlaXRhIG9zIGNvbnRyYXRvcyBlIGFjb3Jkb3MgcHJlZXhpc3RlbnRlcyBkb3MgQXV0b3JlcyBjb20gdGVyY2Vpcm9zLCBjYWJlbmRvIGFvcyBBdXRvcmVzCmluZm9ybWFyIMOgIEluc3RpdHVpw6fDo28gYXMgY29uZGnDp8O1ZXMgZSBvdXRyYXMgcmVzdHJpw6fDtWVzIGltcG9zdGFzIHBvciBlc3RlcyBpbnN0cnVtZW50b3MuCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
title Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
spellingShingle Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
Cordeiro, Taps Aparecida Reis
Chagas
Leishmaniose visceral
Detecção dupla
Imunossensor impedimétrico
Eletropolimerização
Chagas
Visceral leishmaniasis
Dual detection
Impedimetric immunosensor
Electropolymerization
title_short Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
title_full Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
title_fullStr Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
title_full_unstemmed Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
title_sort Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis
author Cordeiro, Taps Aparecida Reis
author_facet Cordeiro, Taps Aparecida Reis
Martins, Helen Rodrigues
Franco, Diego Leoni
Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Cantuaria, Vinicius Lopes
Lana, Marta de
Reis, Alexandre Barbosa
Ferreira, Lucas Franco
author_role author
author2 Martins, Helen Rodrigues
Franco, Diego Leoni
Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Cantuaria, Vinicius Lopes
Lana, Marta de
Reis, Alexandre Barbosa
Ferreira, Lucas Franco
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cordeiro, Taps Aparecida Reis
Martins, Helen Rodrigues
Franco, Diego Leoni
Santos, Fred Luciano Neves
Celedon, Paola Alejandra Fiorani
Cantuaria, Vinicius Lopes
Lana, Marta de
Reis, Alexandre Barbosa
Ferreira, Lucas Franco
dc.subject.other.pt_BR.fl_str_mv Chagas
Leishmaniose visceral
Detecção dupla
Imunossensor impedimétrico
Eletropolimerização
topic Chagas
Leishmaniose visceral
Detecção dupla
Imunossensor impedimétrico
Eletropolimerização
Chagas
Visceral leishmaniasis
Dual detection
Impedimetric immunosensor
Electropolymerization
dc.subject.en.pt_BR.fl_str_mv Chagas
Visceral leishmaniasis
Dual detection
Impedimetric immunosensor
Electropolymerization
description Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15).
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-28T12:53:03Z
dc.date.available.fl_str_mv 2020-10-28T12:53:03Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44242
dc.identifier.issn.pt_BR.fl_str_mv 0956-5663
dc.identifier.doi.none.fl_str_mv 10.1016/j.bios.2020.112573
identifier_str_mv CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020.
0956-5663
10.1016/j.bios.2020.112573
url https://www.arca.fiocruz.br/handle/icict/44242
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44242/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44242/2/Cordeiro%2c%20T.A.R.%20Impedimetric%20immunosensor....pdf
https://www.arca.fiocruz.br/bitstream/icict/44242/3/Cordeiro%2c%20T.A.R.%20Impedimetric%20immunosensor....pdf.txt
bitstream.checksum.fl_str_mv 36b51ef91c52b5338d9d29ba0cc807bc
0fa82fb7681d38814e282fca47cc5c80
8d1b69dd9bdc9df4a8073c7a8193c7af
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325089706442752

Registros relacionados