Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Vitor Márcio
Data de Publicação: 2019
Outros Autores: Miranda, Julia Bahia, Marcelino, Andreza Pain, Andrade, Hélida Monteiro de, Reis, Ilka Afonso, Cardoso, Mariana Santos, Gontijo, Célia Maria Ferreira, Paz, Gustavo Fontes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37960
Resumo: Andreza Pain Marcelino. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
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spelling Ribeiro, Vitor MárcioMiranda, Julia BahiaMarcelino, Andreza PainAndrade, Hélida Monteiro deReis, Ilka AfonsoCardoso, Mariana SantosGontijo, Célia Maria FerreiraPaz, Gustavo Fontes2019-12-16T12:44:19Z2019-12-16T12:44:19Z2019RIBEIRO, Vitor Márcio et al. Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs. Veterinary Parasitology, v. 274, p. 1-6, 2019.0304-4017https://www.arca.fiocruz.br/handle/icict/3796010.1016/j.vetpar.2019.08.014Andreza Pain Marcelino. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.2020-12-16Santo Agostinho Hospital Veterinário. Belo Horizonte, MG, Brasil / Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, Brazil / Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Estatística. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brasil.Visceral leishmaniasis (VL) is a zoonosis caused by the parasite Leishmania infantum and the dog is its main reservoir in rural and urban areas. The diagnosis of infection is mainly based on the presence of anti-Leishmania IgG antibodies in the serum of infected dogs. In this study, the sensitivity and specificity of qualitative rapid tests (RTs) dual path platform (DPP) Bio-Manguinhos, rapid enzyme-linked immunosorbent assay (ELISA) IDEXX, Kalazar Detect and ALERE, as well as quantitative ELISA Bio-Manguinhos and in-house indirect immunofluorescence assay (IFA) tests were analyzed in sera from infected and uninfected dogs. Serial dilutions of the in-house IFA were compared with RTs and ELISA Bio-Manguinhos. The results showed that none of the tests reached 100% sensitivity and specificity. There was no statistical difference between the analyzed RTs. The most sensitive test was the DPP Bio-Manguinhos (97.9%), while the rapid ELISA IDEXX showed higher specificity (100%). In the treatment setting of infected and/or diseased animals, quantitative tests for monitoring the evolution of antibody titers are required, which indicates the maintenance of in-house IFA in animal handling. Furthermore, we demonstrate that the RTs present higher sensitivity in serum samples with superior antibody titers obtained in the in-house IFA. However, the RTs exhibited false negatives in samples with low titers of antibodies. Among the RTs, only the DPP Bio-Manguinhos presented better performance in this situation. Therefore, the use of RTs for the diagnosis of VL in dogs with low titers of antibodies, such as asymptomatic, should be carefully evaluated.engElsevierPerformance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCanine leishmaniasisSerological diagnosisRapid testsEnzyme-linked immunosorbent assayIndirect immunofluorescence assayinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
title Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
spellingShingle Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
Ribeiro, Vitor Márcio
Canine leishmaniasis
Serological diagnosis
Rapid tests
Enzyme-linked immunosorbent assay
Indirect immunofluorescence assay
title_short Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
title_full Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
title_fullStr Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
title_full_unstemmed Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
title_sort Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs
author Ribeiro, Vitor Márcio
author_facet Ribeiro, Vitor Márcio
Miranda, Julia Bahia
Marcelino, Andreza Pain
Andrade, Hélida Monteiro de
Reis, Ilka Afonso
Cardoso, Mariana Santos
Gontijo, Célia Maria Ferreira
Paz, Gustavo Fontes
author_role author
author2 Miranda, Julia Bahia
Marcelino, Andreza Pain
Andrade, Hélida Monteiro de
Reis, Ilka Afonso
Cardoso, Mariana Santos
Gontijo, Célia Maria Ferreira
Paz, Gustavo Fontes
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Vitor Márcio
Miranda, Julia Bahia
Marcelino, Andreza Pain
Andrade, Hélida Monteiro de
Reis, Ilka Afonso
Cardoso, Mariana Santos
Gontijo, Célia Maria Ferreira
Paz, Gustavo Fontes
dc.subject.en.pt_BR.fl_str_mv Canine leishmaniasis
Serological diagnosis
Rapid tests
Enzyme-linked immunosorbent assay
Indirect immunofluorescence assay
topic Canine leishmaniasis
Serological diagnosis
Rapid tests
Enzyme-linked immunosorbent assay
Indirect immunofluorescence assay
description Andreza Pain Marcelino. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-12-16T12:44:19Z
dc.date.available.fl_str_mv 2019-12-16T12:44:19Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Vitor Márcio et al. Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs. Veterinary Parasitology, v. 274, p. 1-6, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37960
dc.identifier.issn.pt_BR.fl_str_mv 0304-4017
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetpar.2019.08.014
identifier_str_mv RIBEIRO, Vitor Márcio et al. Performance of different serological tests in the diagnosis of natural infection by Leishmania infantum in dogs. Veterinary Parasitology, v. 274, p. 1-6, 2019.
0304-4017
10.1016/j.vetpar.2019.08.014
url https://www.arca.fiocruz.br/handle/icict/37960
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/37960/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/37960/2/ve_Ribeiro_Vitor_etal_INI_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/37960/3/ve_Ribeiro_Vitor_etal_INI_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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