Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics

Detalhes bibliográficos
Autor(a) principal: Veras, Dyana Leal
Data de Publicação: 2015
Outros Autores: Lopes, Ana Catarina de Souza, da Silva, Grasielle Vaz, Gonçalves, Gabriel Gazzoni Araújo, de Freitas, Catarina Fernandes, de Lima, Fernanda Cristina Gomes, Maciel, Maria Amélia Vieira, Feitosa, Ana Paula Sampaio, Alves, Luiz Carlos, Brayner, Fábio André
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/21030
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil
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spelling Veras, Dyana LealLopes, Ana Catarina de Souzada Silva, Grasielle VazGonçalves, Gabriel Gazzoni Araújode Freitas, Catarina Fernandesde Lima, Fernanda Cristina GomesMaciel, Maria Amélia VieiraFeitosa, Ana Paula SampaioAlves, Luiz CarlosBrayner, Fábio André2017-09-15T19:56:54Z2017-09-15T19:56:54Z2015VERAS, D. L. et al. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. TheScientificWorldJournal, v. 2015, p. 572128, 2015.1537-744Xhttps://www.arca.fiocruz.br/handle/icict/2103010.1155/2015/572128porGenes BacterianosAntibacterianosKlebsiella pneumoniaeMicrobiotaAnti-Bacterial AgentsGenes, BacterialHumansKlebsiella pneumoniaedrug effectsgeneticsisolation & purificationultrastructureMicrobial Sensitivity TestsMicrobiotaSequence Analysis, DNAbeta-LactamasesGenes BacterianosAntibacterianosHumanosKlebsiella pneumoniaeMicrobiotaAnálise de Sequência de DNAbeta-LactamasesUltrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibioticsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilThe aim of this study was to characterize the ultrastructural effects caused by β-lactam antibiotics in Klebsiella pneumoniae isolates. Three K. pneumoniae clinical isolates were selected for the study with resistance profiles for third-generation cephalosporins, aztreonam, and/or imipenem and with different resistance genes for extended-spectrum β-lactamases (ESBL) or Klebsiella pneumoniae carbapenemase (KPC). Two K. pneumoniae isolates obtained from the microbiota, which were both resistant to amoxicillin and ampicillin, were also analyzed. In accordance with the susceptibility profile, the clinical isolates were subjected to subminimum inhibitory concentrations (sub-MICs) of cefotaxime, ceftazidime, aztreonam, and imipenem and the isolates from the microbiota to ampicillin and amoxicillin, for analysis by means of scanning and transmission electron microscopy. The K. pneumoniae isolates showed different morphological and ultrastructural changes after subjection to β-lactams tested at different concentrations, such as cell filamentation, loss of cytoplasmic material, and deformation of dividing septa. Our results demonstrate that K. pneumoniae isolates harboring different genes that encode for β-lactamases show cell alterations when subjected to different β-lactam antibiotics, thus suggesting that they possess residual activity in vitro, despite the phenotypic resistance presented in the isolates analyzed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/21030/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL26491715 2015 ver-ult.oa.pdf26491715 2015 ver-ult.oa.pdfapplication/pdf8055906https://www.arca.fiocruz.br/bitstream/icict/21030/2/26491715%202015%20ver-ult.oa.pdfd0c5d33236d1445ee513bdbb27fe6714MD52TEXT26491715 2015 ver-ult.oa.pdf.txt26491715 2015 ver-ult.oa.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
title Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
spellingShingle Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
Veras, Dyana Leal
Genes Bacterianos
Antibacterianos
Klebsiella pneumoniae
Microbiota
Anti-Bacterial Agents
Genes, Bacterial
Humans
Klebsiella pneumoniae
drug effects
genetics
isolation & purification
ultrastructure
Microbial Sensitivity Tests
Microbiota
Sequence Analysis, DNA
beta-Lactamases
Genes Bacterianos
Antibacterianos
Humanos
Klebsiella pneumoniae
Microbiota
Análise de Sequência de DNA
beta-Lactamases
title_short Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
title_full Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
title_fullStr Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
title_full_unstemmed Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
title_sort Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics
author Veras, Dyana Leal
author_facet Veras, Dyana Leal
Lopes, Ana Catarina de Souza
da Silva, Grasielle Vaz
Gonçalves, Gabriel Gazzoni Araújo
de Freitas, Catarina Fernandes
de Lima, Fernanda Cristina Gomes
Maciel, Maria Amélia Vieira
Feitosa, Ana Paula Sampaio
Alves, Luiz Carlos
Brayner, Fábio André
author_role author
author2 Lopes, Ana Catarina de Souza
da Silva, Grasielle Vaz
Gonçalves, Gabriel Gazzoni Araújo
de Freitas, Catarina Fernandes
de Lima, Fernanda Cristina Gomes
Maciel, Maria Amélia Vieira
Feitosa, Ana Paula Sampaio
Alves, Luiz Carlos
Brayner, Fábio André
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Veras, Dyana Leal
Lopes, Ana Catarina de Souza
da Silva, Grasielle Vaz
Gonçalves, Gabriel Gazzoni Araújo
de Freitas, Catarina Fernandes
de Lima, Fernanda Cristina Gomes
Maciel, Maria Amélia Vieira
Feitosa, Ana Paula Sampaio
Alves, Luiz Carlos
Brayner, Fábio André
dc.subject.other.pt_BR.fl_str_mv Genes Bacterianos
Antibacterianos
Klebsiella pneumoniae
Microbiota
topic Genes Bacterianos
Antibacterianos
Klebsiella pneumoniae
Microbiota
Anti-Bacterial Agents
Genes, Bacterial
Humans
Klebsiella pneumoniae
drug effects
genetics
isolation & purification
ultrastructure
Microbial Sensitivity Tests
Microbiota
Sequence Analysis, DNA
beta-Lactamases
Genes Bacterianos
Antibacterianos
Humanos
Klebsiella pneumoniae
Microbiota
Análise de Sequência de DNA
beta-Lactamases
dc.subject.en.pt_BR.fl_str_mv Anti-Bacterial Agents
Genes, Bacterial
Humans
Klebsiella pneumoniae
drug effects
genetics
isolation & purification
ultrastructure
Microbial Sensitivity Tests
Microbiota
Sequence Analysis, DNA
beta-Lactamases
dc.subject.decs.pt_BR.fl_str_mv Genes Bacterianos
Antibacterianos
Humanos
Klebsiella pneumoniae
Microbiota
Análise de Sequência de DNA
beta-Lactamases
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2017-09-15T19:56:54Z
dc.date.available.fl_str_mv 2017-09-15T19:56:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VERAS, D. L. et al. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. TheScientificWorldJournal, v. 2015, p. 572128, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/21030
dc.identifier.issn.pt_BR.fl_str_mv 1537-744X
dc.identifier.eissn.none.fl_str_mv 10.1155/2015/572128
identifier_str_mv VERAS, D. L. et al. Ultrastructural Changes in Clinical and Microbiota Isolates of Klebsiella pneumoniae Carriers of Genes bla SHV, bla TEM, bla CTX-M, or bla KPC When Subject to β-Lactam Antibiotics. TheScientificWorldJournal, v. 2015, p. 572128, 2015.
1537-744X
10.1155/2015/572128
url https://www.arca.fiocruz.br/handle/icict/21030
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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