DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes

Detalhes bibliográficos
Autor(a) principal: Mendes-Aguiar, Carolina de O.
Data de Publicação: 2023
Outros Autores: Kitahara-Oliveira, Milene Yoko, Almeida, Ana Cristina Oliveira de, Pereira-Oliveira, Marcia, Oliveira Neto, Manoel Paes de, Pirmez, Claude, Sampaio, Elizabeth Pereira, Gomes-Silva, Adriano, Da-Cruz, Alda Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57316
Resumo: This is a preliminary version of the accepted article
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spelling Mendes-Aguiar, Carolina de O.Kitahara-Oliveira, Milene YokoAlmeida, Ana Cristina Oliveira dePereira-Oliveira, MarciaOliveira Neto, Manoel Paes dePirmez, ClaudeSampaio, Elizabeth PereiraGomes-Silva, AdrianoDa-Cruz, Alda Maria2023-03-09T14:00:39Z2023-03-09T14:00:39Z2023MENDES-AGUIAR, Carolina de O. et al. DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, p. 1-17, Feb. 2023.0074-0276https://www.arca.fiocruz.br/handle/icict/5731610.1590/0074-02760220044This is a preliminary version of the accepted articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio Grande do Norte. Instituto de Medicina Tropical do Rio Grande do Norte. Natal, RN, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia, DMIP. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Micobacterioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Disease, NIH, Bethesda, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Micobacterioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia, DMIP. Rio de Janeiro, RJ, Brasil.BACKGROUND: Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES: In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS: DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mocktransfected (DC-SIGNNEG) were monitored by flow cytometry at 2h, 24h and 48h in coculture. RESULTS: In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DCSIGNlow, while Lb binding was similar in these populations. CONCLUSION: Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DCSIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp infection.engFiocruz/IOChttps://www.arca.fiocruz.br/handle/icict/58973DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDC-SIGN receptorCutaneous leishmaniasisInflammatory infiltrateLeishmania (Viannia) braziliensisLeishmania (Leishmania) amazonensisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57316/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarolineAguiar_ClaudePrimiez_etal_IOC_2023.pdfCarolineAguiar_ClaudePrimiez_etal_IOC_2023.pdfapplication/pdf352620https://www.arca.fiocruz.br/bitstream/icict/57316/2/CarolineAguiar_ClaudePrimiez_etal_IOC_2023.pdf1c00605278d4db6933b354a1e3760153MD52icict/573162023-06-07 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dc.title.en_US.fl_str_mv DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
title DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
spellingShingle DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
Mendes-Aguiar, Carolina de O.
DC-SIGN receptor
Cutaneous leishmaniasis
Inflammatory infiltrate
Leishmania (Viannia) braziliensis
Leishmania (Leishmania) amazonensis
title_short DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
title_full DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
title_fullStr DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
title_full_unstemmed DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
title_sort DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes
author Mendes-Aguiar, Carolina de O.
author_facet Mendes-Aguiar, Carolina de O.
Kitahara-Oliveira, Milene Yoko
Almeida, Ana Cristina Oliveira de
Pereira-Oliveira, Marcia
Oliveira Neto, Manoel Paes de
Pirmez, Claude
Sampaio, Elizabeth Pereira
Gomes-Silva, Adriano
Da-Cruz, Alda Maria
author_role author
author2 Kitahara-Oliveira, Milene Yoko
Almeida, Ana Cristina Oliveira de
Pereira-Oliveira, Marcia
Oliveira Neto, Manoel Paes de
Pirmez, Claude
Sampaio, Elizabeth Pereira
Gomes-Silva, Adriano
Da-Cruz, Alda Maria
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendes-Aguiar, Carolina de O.
Kitahara-Oliveira, Milene Yoko
Almeida, Ana Cristina Oliveira de
Pereira-Oliveira, Marcia
Oliveira Neto, Manoel Paes de
Pirmez, Claude
Sampaio, Elizabeth Pereira
Gomes-Silva, Adriano
Da-Cruz, Alda Maria
dc.subject.en.en_US.fl_str_mv DC-SIGN receptor
Cutaneous leishmaniasis
Inflammatory infiltrate
Leishmania (Viannia) braziliensis
Leishmania (Leishmania) amazonensis
topic DC-SIGN receptor
Cutaneous leishmaniasis
Inflammatory infiltrate
Leishmania (Viannia) braziliensis
Leishmania (Leishmania) amazonensis
description This is a preliminary version of the accepted article
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-03-09T14:00:39Z
dc.date.available.fl_str_mv 2023-03-09T14:00:39Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MENDES-AGUIAR, Carolina de O. et al. DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, p. 1-17, Feb. 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57316
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760220044
identifier_str_mv MENDES-AGUIAR, Carolina de O. et al. DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, p. 1-17, Feb. 2023.
0074-0276
10.1590/0074-02760220044
url https://www.arca.fiocruz.br/handle/icict/57316
dc.language.iso.fl_str_mv eng
language eng
dc.relation.hasversion.none.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58973
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dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57316/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57316/2/CarolineAguiar_ClaudePrimiez_etal_IOC_2023.pdf
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