Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Schallig, Henk D. F. H.
Data de Publicação: 2002
Outros Autores: Cavalheiro, Marilene Canto, Silva, Eduardo S. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44226
Resumo: KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute). Biomedical Research. Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands.
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spelling Schallig, Henk D. F. H.Cavalheiro, Marilene CantoSilva, Eduardo S. da2020-10-27T14:26:17Z2020-10-27T14:26:17Z2002SCHALLIG, Henk D. F. H.; CAVALHEIRO, Marilene Canto; SILVA, Eduardo S. da. Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 7, p. 1015-1018, Oct. 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4422610.1590/S0074-027620020007000151678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Leishmaniose VisceralDiagnósticoLeishmania chagasiHumanosTesT de aglutinação diretaTeste de vareta rK39BrasilVisceral leishmaniasisDirect agglutination tesTrK39 dipstick testLeishmania chagasiDiagnosisHumanBrazilEvaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleKIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute). Biomedical Research. Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado de Minas Gerais. Fundação Educacional de Divinópolis. Centro de Pós-graduação e Pesquisas. Divinópolis, MG Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Leishmanioses. Belo Horizonte, MG, Brasil.The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients with other confirmed infectious diseases (n = 42). The DAT had a sensitivity and a specificity of 100%. The rK39 had a sensitivity of 85.7% and a specificity of 82%. Both tests were also used to screen blood samples of confirmed VL patients (n = 15) and serum samples of VL suspects (n = 61). The DAT found all blood samples of confirmed VL patients positive and tested 98.4% of the serum samples of the VL suspects positive. In contrast, rK39 detected in 9/15 blood samples (60%) antibodies against Leishmania chagasi and found 85.3% of the serum samples of the suspected patients positive. Although the rK39 dipstick is more rapid and user friendlier than the DAT, the latter has a superior sensitivity and specificity. Furthermore, the reagents used for DAT do not require cold storage, whereas the buffer of the rK39 must be stored at 4oC. Therefore, the DAT is the most suitable test for the sero-diagnosis of VL under field conditions.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44226/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarileneCanto_Cavalheiro_etal_IOC_2002.pdfMarileneCanto_Cavalheiro_etal_IOC_2002.pdfapplication/pdf45118https://www.arca.fiocruz.br/bitstream/icict/44226/2/MarileneCanto_Cavalheiro_etal_IOC_2002.pdfb285d82852285d206fef592387a44101MD52TEXTMarileneCanto_Cavalheiro_etal_IOC_2002.pdf.txtMarileneCanto_Cavalheiro_etal_IOC_2002.pdf.txtExtracted texttext/plain20652https://www.arca.fiocruz.br/bitstream/icict/44226/3/MarileneCanto_Cavalheiro_etal_IOC_2002.pdf.txt563239e5ad71a40f41e284ed73db8000MD53icict/442262020-10-28 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dc.title.pt_BR.fl_str_mv Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
title Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
spellingShingle Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
Schallig, Henk D. F. H.
Leishmaniose Visceral
Diagnóstico
Leishmania chagasi
Humanos
TesT de aglutinação direta
Teste de vareta rK39
Brasil
Visceral leishmaniasis
Direct agglutination tesT
rK39 dipstick test
Leishmania chagasi
Diagnosis
Human
Brazil
title_short Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
title_full Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
title_fullStr Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
title_full_unstemmed Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
title_sort Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis
author Schallig, Henk D. F. H.
author_facet Schallig, Henk D. F. H.
Cavalheiro, Marilene Canto
Silva, Eduardo S. da
author_role author
author2 Cavalheiro, Marilene Canto
Silva, Eduardo S. da
author2_role author
author
dc.contributor.author.fl_str_mv Schallig, Henk D. F. H.
Cavalheiro, Marilene Canto
Silva, Eduardo S. da
dc.subject.other.pt_BR.fl_str_mv Leishmaniose Visceral
Diagnóstico
Leishmania chagasi
Humanos
TesT de aglutinação direta
Teste de vareta rK39
Brasil
topic Leishmaniose Visceral
Diagnóstico
Leishmania chagasi
Humanos
TesT de aglutinação direta
Teste de vareta rK39
Brasil
Visceral leishmaniasis
Direct agglutination tesT
rK39 dipstick test
Leishmania chagasi
Diagnosis
Human
Brazil
dc.subject.en.pt_BR.fl_str_mv Visceral leishmaniasis
Direct agglutination tesT
rK39 dipstick test
Leishmania chagasi
Diagnosis
Human
Brazil
description KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute). Biomedical Research. Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-10-27T14:26:17Z
dc.date.available.fl_str_mv 2020-10-27T14:26:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHALLIG, Henk D. F. H.; CAVALHEIRO, Marilene Canto; SILVA, Eduardo S. da. Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 7, p. 1015-1018, Oct. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44226
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000700015
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SCHALLIG, Henk D. F. H.; CAVALHEIRO, Marilene Canto; SILVA, Eduardo S. da. Evaluation of the Direct Agglutination Test and the rK39 Dipstick Test for the Sero-diagnosis of Visceral Leishmaniasis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 7, p. 1015-1018, Oct. 2002.
0074-0276
10.1590/S0074-02762002000700015
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44226
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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