Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis

Bibliographic Details
Main Author: Pinto, P. Faria
Publication Date: 2006
Other Authors: Meirelles, M. N. L., Lenzi, Henrique Leonel, Mota, Ester Maria, Penido, M. L. O., Coelho, Paulo Marcos Zach, Vasconcelos, Eveline Gomes
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/3746
Summary: Acknowledgments to Dr Jair AK Aguiar for revising the manuscript.
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spelling Pinto, P. FariaMeirelles, M. N. L.Lenzi, Henrique LeonelMota, Ester MariaPenido, M. L. O.Coelho, Paulo Marcos ZachVasconcelos, Eveline Gomes2012-01-27T14:20:46Z2012-01-27T14:20:46Z2006FARIA-PINTO, P. et al. Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis. Memórias do Instituto Oswaldo Cruz. v.101, supl.1, p. 359-363, 2006.0074-0276https://www.arca.fiocruz.br/handle/icict/374610.1590/S0074-02762006000900059Acknowledgments to Dr Jair AK Aguiar for revising the manuscript.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Célula. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil.We have previously showed that Schistosoma mansoni ATP-diphosphohydrolase and Solanum tuberosum potato apyrase share epitopes and the vegetable protein has immunostimulatory properties. Here, it was verified the in situ cross-immunoreactivity between mice NTPDases and anti-potato apyrase antibodies produced in rabbits, using confocal microscopy. Liver samples were taken from Swiss Webster mouse 8 weeks after infection with S. mansoni cercariae, and anti-potato apyrase and TRITC-conjugated anti-rabbit IgG antibody were tested on cryostat sections. The results showed that S. mansoni egg ATP diphosphohydrolase isoforms, developed by anti-potato apyrase, are expressed in miracidial and egg structures, and not in granulomatous cells and hepatic structures (hepatocytes, bile ducts, and blood vessels). Therefore, purified potato apyrase when inoculated in rabbit generates polyclonal sera containing anti-apyrase antibodies that are capable of recognizing specifically S. mansoni ATP diphosphohydrolase epitopes, but not proteins from mammalian tissues, suggesting that autoantibodies are not induced during potato apyrase immunization. A phylogenetic tree obtained for the NTPDase family showed that potato apyrase had lower homology with mammalian NTPDases 1-4, 7, and 8. Further analysis of potato apyrase epitopes could implement their potential use in schistosomiasis experimental modelsengFundação Oswaldo Cruz. Instituto Oswaldo CruzCross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCross-immunoreactivitySchistosoma mansoni ATP diphosphohydrolaseMammalian NTPDasesPotato apyrase - granulomaAdenosina TrifosfatasesImunologiaApiraseSchistosoma mansoniEsquistossomose mansoniAdenosina TrifosfatasesDados de Sequência MolecularModelos Animais de Doençasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCross-immunoreactivity between anti-potato apyrase antibodies.pdfCross-immunoreactivity between anti-potato apyrase antibodies.pdfapplication/pdf1082530https://www.arca.fiocruz.br/bitstream/icict/3746/1/Cross-immunoreactivity%20between%20anti-potato%20apyrase%20antibodies.pdf5c5e0325b0a802ea4936ed0cccbde9deMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3746/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTCross-immunoreactivity between anti-potato apyrase antibodies.pdf.txtCross-immunoreactivity between anti-potato apyrase antibodies.pdf.txtExtracted texttext/plain17995https://www.arca.fiocruz.br/bitstream/icict/3746/5/Cross-immunoreactivity%20between%20anti-potato%20apyrase%20antibodies.pdf.txta6ef848658258216f413b4ba761c1b09MD55THUMBNAILCross-immunoreactivity between anti-potato apyrase antibodies.pdf.jpgCross-immunoreactivity between anti-potato apyrase antibodies.pdf.jpgGenerated Thumbnailimage/jpeg2228https://www.arca.fiocruz.br/bitstream/icict/3746/4/Cross-immunoreactivity%20between%20anti-potato%20apyrase%20antibodies.pdf.jpg6bf0344270802cc2a68ad921e859caaaMD54icict/37462018-04-06 11:25:52.046oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T14:25:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
title Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
spellingShingle Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
Pinto, P. Faria
Cross-immunoreactivity
Schistosoma mansoni ATP diphosphohydrolase
Mammalian NTPDases
Potato apyrase - granuloma
Adenosina Trifosfatases
Imunologia
Apirase
Schistosoma mansoni
Esquistossomose mansoni
Adenosina Trifosfatases
Dados de Sequência Molecular
Modelos Animais de Doenças
title_short Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
title_full Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
title_fullStr Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
title_full_unstemmed Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
title_sort Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis
author Pinto, P. Faria
author_facet Pinto, P. Faria
Meirelles, M. N. L.
Lenzi, Henrique Leonel
Mota, Ester Maria
Penido, M. L. O.
Coelho, Paulo Marcos Zach
Vasconcelos, Eveline Gomes
author_role author
author2 Meirelles, M. N. L.
Lenzi, Henrique Leonel
Mota, Ester Maria
Penido, M. L. O.
Coelho, Paulo Marcos Zach
Vasconcelos, Eveline Gomes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto, P. Faria
Meirelles, M. N. L.
Lenzi, Henrique Leonel
Mota, Ester Maria
Penido, M. L. O.
Coelho, Paulo Marcos Zach
Vasconcelos, Eveline Gomes
dc.subject.en.pt_BR.fl_str_mv Cross-immunoreactivity
Schistosoma mansoni ATP diphosphohydrolase
Mammalian NTPDases
Potato apyrase - granuloma
topic Cross-immunoreactivity
Schistosoma mansoni ATP diphosphohydrolase
Mammalian NTPDases
Potato apyrase - granuloma
Adenosina Trifosfatases
Imunologia
Apirase
Schistosoma mansoni
Esquistossomose mansoni
Adenosina Trifosfatases
Dados de Sequência Molecular
Modelos Animais de Doenças
dc.subject.decs.pt_BR.fl_str_mv Adenosina Trifosfatases
Imunologia
Apirase
Schistosoma mansoni
Esquistossomose mansoni
Adenosina Trifosfatases
Dados de Sequência Molecular
Modelos Animais de Doenças
description Acknowledgments to Dr Jair AK Aguiar for revising the manuscript.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2012-01-27T14:20:46Z
dc.date.available.fl_str_mv 2012-01-27T14:20:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA-PINTO, P. et al. Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis. Memórias do Instituto Oswaldo Cruz. v.101, supl.1, p. 359-363, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3746
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000900059
identifier_str_mv FARIA-PINTO, P. et al. Cross-immunoreactivity between anti-potato apyrase antibodies and mammalian ATP diphosphohydrolases: potential use of the vegetal protein in experimental schistosomiasis. Memórias do Instituto Oswaldo Cruz. v.101, supl.1, p. 359-363, 2006.
0074-0276
10.1590/S0074-02762006000900059
url https://www.arca.fiocruz.br/handle/icict/3746
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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