Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8466 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa |
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Santos, Théo de AraújoRodríguez, Nilda EPontes, Sara MouraDixt, Upasna GaurAbánades, Daniel RuizBozza, Patrícia TorresWilson, Mary EditheBorges, Valeria de Matos2014-09-29T16:39:00Z2014-09-29T16:39:00Z2014SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014.0022-1899https://www.arca.fiocruz.br/handle/icict/846610.1093/infdis/jiu299engOxford University PressRole of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, IowaUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, BrasilUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaCentro de Investigaciones Biológicas, CSIC. Department of Chemical and Physical Biology. Madrid, SpainFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversity of Iowa and the Iowa City VA Medical Center. Iowa City, IowaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute for Investigation in Immunology, iii-INCT. National Institute of Science and Technology. São Paulo, SP, BrasilLipid bodies (LB; lipid droplets) are cytoplasmic organelles involved in lipid metabolism. Mammalian LBs display an important role in host-pathogen interactions, but the role of parasite LBs in biosynthesis of prostaglandin F2α (PGF2α) has not been investigated. We report herein that LBs increased in abundance during development of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2α synthase (PGFS). The amount of parasite LBs and PGF2α were modulated by exogenous arachidonic acid. During macrophage infection, LBs were restricted to parasites inside the parasitophorous vacuoles (PV). We detected PGF2α receptor (FP) on the Leishmania PV surface. The blockage of FP with AL8810, a selective antagonist, hampered Leishmania infection, whereas the irreversible inhibition of cyclooxygenase with aspirin increased the parasite burden. These data demonstrate novel functions for parasite-derived LBs and PGF2α in the cellular metabolism of Leishmania and its evasion of the host immune response.Leishmania infantum chagasiLipid dropletsProstaglandin F2αPGF2α synthaseMacrophage infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8466/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSantos TA Role....pdfSantos TA Role....pdfapplication/pdf797145https://www.arca.fiocruz.br/bitstream/icict/8466/2/Santos%20TA%20Role....pdff1a53c60daadd7bee2a4bf3a5e90fd71MD52TEXTSantos TA Role....pdf.txtSantos TA Role....pdf.txtExtracted texttext/plain43968https://www.arca.fiocruz.br/bitstream/icict/8466/3/Santos%20TA%20Role....pdf.txtb8db70de7a0f3baa04a50548c245f733MD53icict/84662023-09-14 15:53:26.491oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T18:53:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| title |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| spellingShingle |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence Santos, Théo de Araújo Leishmania infantum chagasi Lipid droplets Prostaglandin F2α PGF2α synthase Macrophage infection |
| title_short |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| title_full |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| title_fullStr |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| title_full_unstemmed |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| title_sort |
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence |
| author |
Santos, Théo de Araújo |
| author_facet |
Santos, Théo de Araújo Rodríguez, Nilda E Pontes, Sara Moura Dixt, Upasna Gaur Abánades, Daniel Ruiz Bozza, Patrícia Torres Wilson, Mary Edithe Borges, Valeria de Matos |
| author_role |
author |
| author2 |
Rodríguez, Nilda E Pontes, Sara Moura Dixt, Upasna Gaur Abánades, Daniel Ruiz Bozza, Patrícia Torres Wilson, Mary Edithe Borges, Valeria de Matos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Théo de Araújo Rodríguez, Nilda E Pontes, Sara Moura Dixt, Upasna Gaur Abánades, Daniel Ruiz Bozza, Patrícia Torres Wilson, Mary Edithe Borges, Valeria de Matos |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania infantum chagasi Lipid droplets Prostaglandin F2α PGF2α synthase Macrophage infection |
| topic |
Leishmania infantum chagasi Lipid droplets Prostaglandin F2α PGF2α synthase Macrophage infection |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / University of Iowa and the Iowa City VA Medical Center. Iowa City, Iowa |
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2014 |
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2014-09-29T16:39:00Z |
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2014-09-29T16:39:00Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014. |
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https://www.arca.fiocruz.br/handle/icict/8466 |
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0022-1899 |
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10.1093/infdis/jiu299 |
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SANTOS, T. A. et al. Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence. Journal of Infectious Diseases, jun. 2014. 0022-1899 10.1093/infdis/jiu299 |
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Oxford University Press |
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Oxford University Press |
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