Avaliação de polimorfismos genéticos em HIF1A e expressão da proteína HIF-1α durante a compressão ortodôntica

• Main Author: Teodoro, Vinicius Broska
• Publication Date: 2020
• Format: Master thesis
• Language: por
• Source: Repositório do Centro Universitário Braz Cubas
• Download full: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2120
• topic: Odontologia; Hipóxia; Técnicas de movimentação dentária; Polimorfismo genético; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

Many genes and signaling molecules are involved in orthodontic tooth movement (OTM) with mechanically and hypoxically stabilized HIF-1α having been shown to play a decisive role in PDL signaling during OTM. Therefore, this in vitro study aimed to investigate, if genetic polymorphisms in HIF1A (Hypoxia-inducible factor α-subunits) influence the expression pattern of HIF-1α protein during simulated orthodontic compressive pressure. Samples from human periodontal ligament (hPDL) fibroblasts were used and their DNA was genotyped using real time PCR for the genetic polymorphisms rs2301113 and rs2057482 in HIF1A. For cell culture and protein expression experiments, six hPDL fibroblast cell lines were selected based on the patients’ genotype. To simulate orthodontic compressive pressure in hPDL fibroblasts, a force of 2 g/cm2 was applied under cell culture conditions for 48h. Protein expression was evaluated by Western Blot. Paired t-tests were used to compare HIF-1α expression with and without compressive pressure application and T tests to compare expression among the genotypes in rs2057482 and rs2301113 (p<0.05). The expression of HIF-1α protein was significantly enhanced by compressive pressure application regardless of the genotype (p<0.0001). The genotypes in the genetic polymorphisms rs2301113 and rs2057482 were not associated with HIF-1α protein expression. In conclusion, the genetic polymorphisms in HIF1A did not impact on the expression pattern of HIF-1α protein by hPDL fibroblasts during simulated orthodontic compressive pressure.