Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media

Detalhes bibliográficos
Autor(a) principal: Ristow,Paula
Data de Publicação: 2006
Outros Autores: Silva,Marlei Gomes, Fonseca,Leila de Souza, Lilenbaum,Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2006000100001
Resumo: Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map.
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spelling Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and mediaParatuberculosisbovinediagnosismycobacteriumParatuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map.Colégio Brasileiro de Patologia Animal - CBPA2006-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2006000100001Pesquisa Veterinária Brasileira v.26 n.1 2006reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2006000100001info:eu-repo/semantics/openAccessRistow,PaulaSilva,Marlei GomesFonseca,Leila de SouzaLilenbaum,Waltereng2006-10-06T00:00:00Zoai:scielo:S0100-736X2006000100001Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2006-10-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
title Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
spellingShingle Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
Ristow,Paula
Paratuberculosis
bovine
diagnosis
mycobacterium
title_short Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
title_full Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
title_fullStr Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
title_full_unstemmed Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
title_sort Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
author Ristow,Paula
author_facet Ristow,Paula
Silva,Marlei Gomes
Fonseca,Leila de Souza
Lilenbaum,Walter
author_role author
author2 Silva,Marlei Gomes
Fonseca,Leila de Souza
Lilenbaum,Walter
author2_role author
author
author
dc.contributor.author.fl_str_mv Ristow,Paula
Silva,Marlei Gomes
Fonseca,Leila de Souza
Lilenbaum,Walter
dc.subject.por.fl_str_mv Paratuberculosis
bovine
diagnosis
mycobacterium
topic Paratuberculosis
bovine
diagnosis
mycobacterium
description Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map.
publishDate 2006
dc.date.none.fl_str_mv 2006-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2006000100001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2006000100001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2006000100001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.26 n.1 2006
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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