Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006 |
Resumo: | An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses. |
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Development and standardization of an indirect ELISA for the serological diagnosis of classical swine feverClassical swine feverclassical swine fever virusELISAserologyAn indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.Colégio Brasileiro de Patologia Animal - CBPA1999-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006Pesquisa Veterinária Brasileira v.19 n.3-4 1999reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X1999000300006info:eu-repo/semantics/openAccessParedes,Julio Cesar MuñozOliveira,Liliane GuimarãesBraga,Alexandre de CarvalhoTrevisol,Iara MariaRoehe,Paulo Micheleng2000-02-25T00:00:00Zoai:scielo:S0100-736X1999000300006Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2000-02-25T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
title |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
spellingShingle |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever Paredes,Julio Cesar Muñoz Classical swine fever classical swine fever virus ELISA serology |
title_short |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
title_full |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
title_fullStr |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
title_full_unstemmed |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
title_sort |
Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever |
author |
Paredes,Julio Cesar Muñoz |
author_facet |
Paredes,Julio Cesar Muñoz Oliveira,Liliane Guimarães Braga,Alexandre de Carvalho Trevisol,Iara Maria Roehe,Paulo Michel |
author_role |
author |
author2 |
Oliveira,Liliane Guimarães Braga,Alexandre de Carvalho Trevisol,Iara Maria Roehe,Paulo Michel |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Paredes,Julio Cesar Muñoz Oliveira,Liliane Guimarães Braga,Alexandre de Carvalho Trevisol,Iara Maria Roehe,Paulo Michel |
dc.subject.por.fl_str_mv |
Classical swine fever classical swine fever virus ELISA serology |
topic |
Classical swine fever classical swine fever virus ELISA serology |
description |
An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X1999000300006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.19 n.3-4 1999 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122227419185152 |