Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000400586 |
Resumo: | ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. In our study, we showed that MALDI-TOF MS coupled with Biotyper may be an alternative method for large-scale identification of bacteria isolated from milk samples compared to classical microbiological routine protocols. |
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Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass SpectrometryRapid identificationbovine mastitispathogensMALDI-TOF MSMass Spectrometryprotein fingerprintingsubclinical mastitiscattlebacteriosesABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. In our study, we showed that MALDI-TOF MS coupled with Biotyper may be an alternative method for large-scale identification of bacteria isolated from milk samples compared to classical microbiological routine protocols.Colégio Brasileiro de Patologia Animal - CBPA2018-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000400586Pesquisa Veterinária Brasileira v.38 n.4 2018reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-4821info:eu-repo/semantics/openAccessBraga,Patrícia A.C.Gonçalves,Juliano L.Barreiro,Juliana R.Ferreira,Christina R.Tomazi,TiagoEberlin,Marcos N.Santos,Marcos V.eng2018-06-28T00:00:00Zoai:scielo:S0100-736X2018000400586Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2018-06-28T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
title |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
spellingShingle |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry Braga,Patrícia A.C. Rapid identification bovine mastitis pathogens MALDI-TOF MS Mass Spectrometry protein fingerprinting subclinical mastitis cattle bacterioses |
title_short |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
title_full |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
title_fullStr |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
title_full_unstemmed |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
title_sort |
Rapid identification of bovine mastitis pathogens by MALDI-TOF Mass Spectrometry |
author |
Braga,Patrícia A.C. |
author_facet |
Braga,Patrícia A.C. Gonçalves,Juliano L. Barreiro,Juliana R. Ferreira,Christina R. Tomazi,Tiago Eberlin,Marcos N. Santos,Marcos V. |
author_role |
author |
author2 |
Gonçalves,Juliano L. Barreiro,Juliana R. Ferreira,Christina R. Tomazi,Tiago Eberlin,Marcos N. Santos,Marcos V. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Braga,Patrícia A.C. Gonçalves,Juliano L. Barreiro,Juliana R. Ferreira,Christina R. Tomazi,Tiago Eberlin,Marcos N. Santos,Marcos V. |
dc.subject.por.fl_str_mv |
Rapid identification bovine mastitis pathogens MALDI-TOF MS Mass Spectrometry protein fingerprinting subclinical mastitis cattle bacterioses |
topic |
Rapid identification bovine mastitis pathogens MALDI-TOF MS Mass Spectrometry protein fingerprinting subclinical mastitis cattle bacterioses |
description |
ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able to identify bacteria at the genus, species and even at subspecies level. With the aim of large-scale identification of pathogens causing mastitis by this platform, a total of 305 isolates of bacteria identified from cows with subclinical mastitis were analyzed by conventional microbiological culture (MC) as well as by MALDI-TOF MS coupled with Biotyper data processing. Approximately 89% of the identifications performed by MALDI-TOF MS were consistent with results obtained by MC. From the remaining isolates (11%), 6.3% of isolates were classified as misidentified (discordance for both genus and species level), and 4.7% showed identification agreement at the genus level but not at the species level, being classified as unidentified at species level. The disagreement results were mostly associated with identification of Streptococcus and Enterococcus species probably due to the narrow phenotypic similarity between these two genera. These disagreement results suggest that biochemical assays might be prone to identification errors and, MALDI-TOF MS therefore may be an alternative to overcome incorrect species-specific identification. Standard microbiological methods for bovine mastitis diagnosis are time consuming, laborious and prone to errors for some bacteria genera. In our study, we showed that MALDI-TOF MS coupled with Biotyper may be an alternative method for large-scale identification of bacteria isolated from milk samples compared to classical microbiological routine protocols. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000400586 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018000400586 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-4821 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.38 n.4 2018 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122238339055616 |