Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000600643 |
Resumo: | ABSTRACT: Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood. |
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Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord bloodMSCcaprineflow cytometrystem celllineage differentiationABSTRACT: Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood.Colégio Brasileiro de Patologia Animal - CBPA2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000600643Pesquisa Veterinária Brasileira v.37 n.6 2017reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/s0100-736x2017000600019info:eu-repo/semantics/openAccessMartins,Gabrielle R.Marinho,Rebeca C.Q. Bezerra-Junior,RosivaldoCâmara,Lilia M.C.Albuquerque-Pinto,Luiz C.Teixeira,Maria F.S.eng2017-09-04T00:00:00Zoai:scielo:S0100-736X2017000600643Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2017-09-04T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
title |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
spellingShingle |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood Martins,Gabrielle R. MSC caprine flow cytometry stem cell lineage differentiation |
title_short |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
title_full |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
title_fullStr |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
title_full_unstemmed |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
title_sort |
Isolation, culture and characterization of multipotent mesenchymal stem cells from goat umbilical cord blood |
author |
Martins,Gabrielle R. |
author_facet |
Martins,Gabrielle R. Marinho,Rebeca C. Q. Bezerra-Junior,Rosivaldo Câmara,Lilia M.C. Albuquerque-Pinto,Luiz C. Teixeira,Maria F.S. |
author_role |
author |
author2 |
Marinho,Rebeca C. Q. Bezerra-Junior,Rosivaldo Câmara,Lilia M.C. Albuquerque-Pinto,Luiz C. Teixeira,Maria F.S. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Martins,Gabrielle R. Marinho,Rebeca C. Q. Bezerra-Junior,Rosivaldo Câmara,Lilia M.C. Albuquerque-Pinto,Luiz C. Teixeira,Maria F.S. |
dc.subject.por.fl_str_mv |
MSC caprine flow cytometry stem cell lineage differentiation |
topic |
MSC caprine flow cytometry stem cell lineage differentiation |
description |
ABSTRACT: Mesenchymal stem cells (MSC) reside in small numbers in many adult tissues and organs, and play an active role in the homeostasis of these sites. Goat derived multipotent MSC have been established from bone marrow, adipose tissues and amniotic fluid. Umbilical cord blood (UCB) is considered an important source of these cells. However, the MSC isolation from the goat UCB has not been demonstrated. Therefore, the aim of the present study was to isolate, culture and characterize goat umbilical cord blood derived mesenchymal stem cells. MSC were isolated from UCB by Ficoll-Paque density centrifugation and cultured in DMEM supplemented with 10% or 20% FBS. FACS analysis was performed and induction lineage differentiation was made to characterize these cells. They exhibited two different populations in flow cytometry, and revealed the positive expression of CD90, CD44 and CD105, but negative staining for CD34 in larger cells, and positive stained for CD90 and CD105, but negative for CD44 and CD34 in the smaller cells. MSC from goat UCB showed capability to differentiate into chondrocytes and osteoblasts when incubated with specific differentiation medium. Present study established that goat mesenchymal stem cells can be derived successfully from umbilical cord blood. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000600643 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000600643 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0100-736x2017000600019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.37 n.6 2017 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122237087055872 |