Identification and characterization of Aspergillus fumigatus isolates from broilers

Detalhes bibliográficos
Autor(a) principal: Spanamberg,Andréia
Data de Publicação: 2016
Outros Autores: Ferreiro,Laerte, Machado,Gustavo, Fraga,Cibele Floriano, Araujo,Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000700591
Resumo: Abstract: Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung's samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung's samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of β-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung's samples of broilers.
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spelling Identification and characterization of Aspergillus fumigatus isolates from broilersAspergillus fumigatusbroilersmolecular identificationmultiplex PCRgenotypingAbstract: Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung's samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung's samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of β-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung's samples of broilers.Colégio Brasileiro de Patologia Animal - CBPA2016-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000700591Pesquisa Veterinária Brasileira v.36 n.7 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2016000700005info:eu-repo/semantics/openAccessSpanamberg,AndréiaFerreiro,LaerteMachado,GustavoFraga,Cibele FlorianoAraujo,Ricardoeng2016-09-12T00:00:00Zoai:scielo:S0100-736X2016000700591Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-09-12T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Identification and characterization of Aspergillus fumigatus isolates from broilers
title Identification and characterization of Aspergillus fumigatus isolates from broilers
spellingShingle Identification and characterization of Aspergillus fumigatus isolates from broilers
Spanamberg,Andréia
Aspergillus fumigatus
broilers
molecular identification
multiplex PCR
genotyping
title_short Identification and characterization of Aspergillus fumigatus isolates from broilers
title_full Identification and characterization of Aspergillus fumigatus isolates from broilers
title_fullStr Identification and characterization of Aspergillus fumigatus isolates from broilers
title_full_unstemmed Identification and characterization of Aspergillus fumigatus isolates from broilers
title_sort Identification and characterization of Aspergillus fumigatus isolates from broilers
author Spanamberg,Andréia
author_facet Spanamberg,Andréia
Ferreiro,Laerte
Machado,Gustavo
Fraga,Cibele Floriano
Araujo,Ricardo
author_role author
author2 Ferreiro,Laerte
Machado,Gustavo
Fraga,Cibele Floriano
Araujo,Ricardo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spanamberg,Andréia
Ferreiro,Laerte
Machado,Gustavo
Fraga,Cibele Floriano
Araujo,Ricardo
dc.subject.por.fl_str_mv Aspergillus fumigatus
broilers
molecular identification
multiplex PCR
genotyping
topic Aspergillus fumigatus
broilers
molecular identification
multiplex PCR
genotyping
description Abstract: Aspergillosis is one of the main causes of mortality in birds. The pulmonary system is most frequently affected, with lesions observed in the air sacs and lungs of a wide variety of bird species. The aim of this study was to confirm by molecular methods the identification and the genetic diversity of Aspergillus fumigatus isolates of lung's samples from healthy broilers (Galus galus domesticus). Forty-four (9.5%) isolates of lung's samples were confirmed as A. fumigatus by polymerase chain reaction (PCR) multiplex (amplification of β-tub and rodA gene fragments). Microsatellite typing for A. fumigatus was used to analyse all avian isolates. Among them, 40 genotypes (90.9%) were observed only one time. The results showed a high variability and multiple genotypes of de A. fumigatus collected from lung's samples of broilers.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000700591
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000700591
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2016000700005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.36 n.7 2016
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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