Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000100007 |
Resumo: | The objective of this study was to determine the presence of the colonization factor F42 in 168 strains of Escherichia coli isolated from diarrheic stools of newborn piglets. The presence of F42 in 12 (7.1%) strains was detected with the agglutination test. Through the Polymerase Chain Reaction (PCR) of F42 positive strains, gene encoding enterotoxins (ST-I, ST-II, LT-I and LT-II) were detected. The finding of ST-I/ST-II genes in 50% of the strains, ST-I (16%) and ST-II (25%) indicates a strong association of FC F42 with heat-stable enterotoxins (91%). In contrast, the thermolabile enterotoxin (LT-I and LT-II) genes were not detected. Serogroups of F42 positive strains were determined, serogroup O8 being the most prevalent (41,7%). Other serogroups, as there are O9, O11, O18, O32, O35, O98 and O101, were also identified. Thus, FC F42 was confirmed as an additional factor of virulence in the pathogenesis of porcine colibacillosis. |
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Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrheaEscherichia coliF42pigletsfimbriaediarrheaPCRThe objective of this study was to determine the presence of the colonization factor F42 in 168 strains of Escherichia coli isolated from diarrheic stools of newborn piglets. The presence of F42 in 12 (7.1%) strains was detected with the agglutination test. Through the Polymerase Chain Reaction (PCR) of F42 positive strains, gene encoding enterotoxins (ST-I, ST-II, LT-I and LT-II) were detected. The finding of ST-I/ST-II genes in 50% of the strains, ST-I (16%) and ST-II (25%) indicates a strong association of FC F42 with heat-stable enterotoxins (91%). In contrast, the thermolabile enterotoxin (LT-I and LT-II) genes were not detected. Serogroups of F42 positive strains were determined, serogroup O8 being the most prevalent (41,7%). Other serogroups, as there are O9, O11, O18, O32, O35, O98 and O101, were also identified. Thus, FC F42 was confirmed as an additional factor of virulence in the pathogenesis of porcine colibacillosis.Colégio Brasileiro de Patologia Animal - CBPA2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000100007Pesquisa Veterinária Brasileira v.25 n.1 2005reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2005000100007info:eu-repo/semantics/openAccessPenatti,Mário Paulo A.Silva,Alex S.Valadares,Geórgio F.Leite,Domingos S.eng2005-06-14T00:00:00Zoai:scielo:S0100-736X2005000100007Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2005-06-14T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
title |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
spellingShingle |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea Penatti,Mário Paulo A. Escherichia coli F42 piglets fimbriae diarrhea PCR |
title_short |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
title_full |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
title_fullStr |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
title_full_unstemmed |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
title_sort |
Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea |
author |
Penatti,Mário Paulo A. |
author_facet |
Penatti,Mário Paulo A. Silva,Alex S. Valadares,Geórgio F. Leite,Domingos S. |
author_role |
author |
author2 |
Silva,Alex S. Valadares,Geórgio F. Leite,Domingos S. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Penatti,Mário Paulo A. Silva,Alex S. Valadares,Geórgio F. Leite,Domingos S. |
dc.subject.por.fl_str_mv |
Escherichia coli F42 piglets fimbriae diarrhea PCR |
topic |
Escherichia coli F42 piglets fimbriae diarrhea PCR |
description |
The objective of this study was to determine the presence of the colonization factor F42 in 168 strains of Escherichia coli isolated from diarrheic stools of newborn piglets. The presence of F42 in 12 (7.1%) strains was detected with the agglutination test. Through the Polymerase Chain Reaction (PCR) of F42 positive strains, gene encoding enterotoxins (ST-I, ST-II, LT-I and LT-II) were detected. The finding of ST-I/ST-II genes in 50% of the strains, ST-I (16%) and ST-II (25%) indicates a strong association of FC F42 with heat-stable enterotoxins (91%). In contrast, the thermolabile enterotoxin (LT-I and LT-II) genes were not detected. Serogroups of F42 positive strains were determined, serogroup O8 being the most prevalent (41,7%). Other serogroups, as there are O9, O11, O18, O32, O35, O98 and O101, were also identified. Thus, FC F42 was confirmed as an additional factor of virulence in the pathogenesis of porcine colibacillosis. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000100007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000100007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2005000100007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.25 n.1 2005 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122228189888512 |