Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables
Main Author: | |
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Publication Date: | 2012 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Pesquisa Veterinária Brasileira (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000300002 |
Summary: | Leptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues. |
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Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variablesLeptospira spp.sheeptissue samplesisolationPCRslaughterhouseLeptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues.Colégio Brasileiro de Patologia Animal - CBPA2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000300002Pesquisa Veterinária Brasileira v.32 n.3 2012reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2012000300002info:eu-repo/semantics/openAccessSilva,Rodrigo Costa daCosta,Veruska Maia daShimabukuro,Fabio HirotoRichini-Pereira,Virgínia BodelãoMenozzi,Benedito DonizeteLangoni,Hélioeng2016-12-20T00:00:00Zoai:scielo:S0100-736X2012000300002Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-12-20T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
title |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
spellingShingle |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables Silva,Rodrigo Costa da Leptospira spp. sheep tissue samples isolation PCR slaughterhouse |
title_short |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
title_full |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
title_fullStr |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
title_full_unstemmed |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
title_sort |
Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables |
author |
Silva,Rodrigo Costa da |
author_facet |
Silva,Rodrigo Costa da Costa,Veruska Maia da Shimabukuro,Fabio Hiroto Richini-Pereira,Virgínia Bodelão Menozzi,Benedito Donizete Langoni,Hélio |
author_role |
author |
author2 |
Costa,Veruska Maia da Shimabukuro,Fabio Hiroto Richini-Pereira,Virgínia Bodelão Menozzi,Benedito Donizete Langoni,Hélio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Rodrigo Costa da Costa,Veruska Maia da Shimabukuro,Fabio Hiroto Richini-Pereira,Virgínia Bodelão Menozzi,Benedito Donizete Langoni,Hélio |
dc.subject.por.fl_str_mv |
Leptospira spp. sheep tissue samples isolation PCR slaughterhouse |
topic |
Leptospira spp. sheep tissue samples isolation PCR slaughterhouse |
description |
Leptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000300002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2012000300002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.32 n.3 2012 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122231824252928 |