Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops

Detalhes bibliográficos
Autor(a) principal: Fernandes, Marcelo Ferreira
Data de Publicação: 2003
Outros Autores: Fernandes, Roberta Pereira Miranda, Hungria, Mariangela
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6654
Resumo: The objective of this work was to characterize genetically seven indigenous rhizobial strains from the coastal tableland of Sergipe, Brazil, with high efficiency of biological nitrogen fixation with the pigeonpea (Cajanus cajan) and cowpea (Vigna unguiculata) crops. The DNA amplification with the PCR (polymerase chain reaction) technique and the specific primer BOX indicated a high level of genetic diversity, with all isolates showing unique profiles of DNA. The BOX-PCR analysis also indicated that this method is efficient to characterize strains but not to define rhizobial species. The RFLP (restriction fragment length polymorphism) analysis of the 16S rRNA region and of the intergenic region between the 16S and the 23S rRNA genes with five restriction enzymes, as well as the partial sequencing of the 16S rRNA region allowed the classification of the strains into the genera Bradyrhizobium and Rhizobium. There was an agreement between the results obtained by the analysis of the 16S rRNA region, but one strain differed when the intergenic space was considered. The results obtained with strain R11 showed a high level of genetic diversity when compared to the described rhizobial species, including differences in several bases of the 16S rRNA region, and might indicate a new species.
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spelling Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea cropsCaracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupiCajanus cajan; Vigna unguiculata; molecular biology; nitrogen fixation; genetic markersCajanus cajan; Vigna unguiculata; biologia molecular; fixação de nitrogênio; marcador genéticoThe objective of this work was to characterize genetically seven indigenous rhizobial strains from the coastal tableland of Sergipe, Brazil, with high efficiency of biological nitrogen fixation with the pigeonpea (Cajanus cajan) and cowpea (Vigna unguiculata) crops. The DNA amplification with the PCR (polymerase chain reaction) technique and the specific primer BOX indicated a high level of genetic diversity, with all isolates showing unique profiles of DNA. The BOX-PCR analysis also indicated that this method is efficient to characterize strains but not to define rhizobial species. The RFLP (restriction fragment length polymorphism) analysis of the 16S rRNA region and of the intergenic region between the 16S and the 23S rRNA genes with five restriction enzymes, as well as the partial sequencing of the 16S rRNA region allowed the classification of the strains into the genera Bradyrhizobium and Rhizobium. There was an agreement between the results obtained by the analysis of the 16S rRNA region, but one strain differed when the intergenic space was considered. The results obtained with strain R11 showed a high level of genetic diversity when compared to the described rhizobial species, including differences in several bases of the 16S rRNA region, and might indicate a new species.O objetivo deste trabalho foi caracterizar geneticamente sete estirpes de rizóbios nativos dos tabuleiros costeiros de Sergipe com alta eficiência de fixação biológica do N2 em associação com guandu (Cajanus cajan) e caupi (Vigna unguiculata). A amplificação do DNA pela técnica de PCR (polymerase chain reaction) com o oligonucleotídeo específico BOX indicou um grau elevado de diversidade genética, uma vez que todas as estirpes apresentaram perfis únicos de DNA. A análise por BOX-PCR revelou, ainda, que essa metodologia é eficiente para diferenciar estirpes, mas não para a diferenciação de espécies de rizóbio. Pela técnica do RFLP (restriction fragment length polymorphism) da região do DNA que codifica o gene 16S rRNA e da região intergênica entre os genes 16S e 23S rRNA, com cinco enzimas de restrição, bem como pelo seqüenciamento parcial da região do 16S rRNA, foi possível classificar as estirpes nos gêneros Bradyrhizobium e Rhizobium. Houve coerência entre as análises envolvendo a região do 16S rRNA, mas o agrupamento com uma das estirpes diferiu pela análise do espaço intergênico. Os resultados obtidos com a estirpe R11 indicam variabilidade genética elevada em relação às espécies de rizóbios descritas, inclusive diferindo em diversas bases da região do 16S rRNA, e podem indicar uma nova espécie.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFernandes, Marcelo FerreiraFernandes, Roberta Pereira MirandaHungria, Mariangela2003-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6654Pesquisa Agropecuaria Brasileira; v.38, n.8, ago. 2003; 911-920Pesquisa Agropecuária Brasileira; v.38, n.8, ago. 2003; 911-9201678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6654/3711info:eu-repo/semantics/openAccess2014-08-11T19:22:24Zoai:ojs.seer.sct.embrapa.br:article/6654Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-11T19:22:24Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
Caracterização genética de rizóbios nativos dos tabuleiros costeiros eficientes em culturas do guandu e caupi
title Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
spellingShingle Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
Fernandes, Marcelo Ferreira
Cajanus cajan; Vigna unguiculata; molecular biology; nitrogen fixation; genetic markers
Cajanus cajan; Vigna unguiculata; biologia molecular; fixação de nitrogênio; marcador genético
title_short Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
title_full Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
title_fullStr Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
title_full_unstemmed Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
title_sort Genetic characterization of indigenous rhizobia strains from the coastal tableland efficient for the pigeonpea and cowpea crops
author Fernandes, Marcelo Ferreira
author_facet Fernandes, Marcelo Ferreira
Fernandes, Roberta Pereira Miranda
Hungria, Mariangela
author_role author
author2 Fernandes, Roberta Pereira Miranda
Hungria, Mariangela
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Fernandes, Marcelo Ferreira
Fernandes, Roberta Pereira Miranda
Hungria, Mariangela
dc.subject.por.fl_str_mv Cajanus cajan; Vigna unguiculata; molecular biology; nitrogen fixation; genetic markers
Cajanus cajan; Vigna unguiculata; biologia molecular; fixação de nitrogênio; marcador genético
topic Cajanus cajan; Vigna unguiculata; molecular biology; nitrogen fixation; genetic markers
Cajanus cajan; Vigna unguiculata; biologia molecular; fixação de nitrogênio; marcador genético
description The objective of this work was to characterize genetically seven indigenous rhizobial strains from the coastal tableland of Sergipe, Brazil, with high efficiency of biological nitrogen fixation with the pigeonpea (Cajanus cajan) and cowpea (Vigna unguiculata) crops. The DNA amplification with the PCR (polymerase chain reaction) technique and the specific primer BOX indicated a high level of genetic diversity, with all isolates showing unique profiles of DNA. The BOX-PCR analysis also indicated that this method is efficient to characterize strains but not to define rhizobial species. The RFLP (restriction fragment length polymorphism) analysis of the 16S rRNA region and of the intergenic region between the 16S and the 23S rRNA genes with five restriction enzymes, as well as the partial sequencing of the 16S rRNA region allowed the classification of the strains into the genera Bradyrhizobium and Rhizobium. There was an agreement between the results obtained by the analysis of the 16S rRNA region, but one strain differed when the intergenic space was considered. The results obtained with strain R11 showed a high level of genetic diversity when compared to the described rhizobial species, including differences in several bases of the 16S rRNA region, and might indicate a new species.
publishDate 2003
dc.date.none.fl_str_mv 2003-08-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6654
url https://seer.sct.embrapa.br/index.php/pab/article/view/6654
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6654/3711
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.38, n.8, ago. 2003; 911-920
Pesquisa Agropecuária Brasileira; v.38, n.8, ago. 2003; 911-920
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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