Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs

Detalhes bibliográficos
Autor(a) principal: Piffer, Itamar Antônio
Data de Publicação: 2014
Outros Autores: Ross, Richard Francis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/15894
Resumo: Direct and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of mycoplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test.
id EMBRAPA-4_4aa8d6384d5b3e4131af032274097bc2
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/15894
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungsTécnicas de imunofluorescência para detecção de Mycoplasma hyopneumoniae em pulmões de suínosmycoplasmal pneumonia; enzootic pneumonia; diagnosispneumonia micoplásmica; pneumonia enzoótica; diagnósticoDirect and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of mycoplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test.Compararam-se as técnicas de imunofluorescência direta e indireta e de cultivo na detecção de Mycoplasma hyopneumoniae em pulmões de suínos infectados experimentalmente. Estes pulmões foram obtidos de animais SPF após seis a sete semanas de início de um período de contato com outros animais SPF inoculados experimentalmente com o agente. Uma boa associação foi encontrada entre o isolamento de M. hyopneumoniae, resultados positivos em ambas as técnicas de imunofluorescência e lesões macroscópicas e microscópicas típicas de pneumonia micoplásmica dos suínos. Tanto a imunofluorescência direta como indireta foram igualmente eficientes na detecção de M. hyopneumoniae em secções de pulmão. Uma coloração azo de fundo reduziu a fluorescência inespecífica e possibilitou um bom contraste de cor sem reduzir a sensibilidade do método indireto de imunofluorescência.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPiffer, Itamar AntônioRoss, Richard Francis2014-10-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/15894Pesquisa Agropecuaria Brasileira; v.20, n.8, ago. 1985; 877-882Pesquisa Agropecuária Brasileira; v.20, n.8, ago. 1985; 877-8821678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/15894/9982info:eu-repo/semantics/openAccess2014-04-17T11:58:54Zoai:ojs.seer.sct.embrapa.br:article/15894Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-17T11:58:54Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
Técnicas de imunofluorescência para detecção de Mycoplasma hyopneumoniae em pulmões de suínos
title Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
spellingShingle Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
Piffer, Itamar Antônio
mycoplasmal pneumonia; enzootic pneumonia; diagnosis
pneumonia micoplásmica; pneumonia enzoótica; diagnóstico
title_short Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
title_full Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
title_fullStr Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
title_full_unstemmed Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
title_sort Immunofluorescence technique for detection of Mycoplasma hyopneumoniae in swine lungs
author Piffer, Itamar Antônio
author_facet Piffer, Itamar Antônio
Ross, Richard Francis
author_role author
author2 Ross, Richard Francis
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Piffer, Itamar Antônio
Ross, Richard Francis
dc.subject.por.fl_str_mv mycoplasmal pneumonia; enzootic pneumonia; diagnosis
pneumonia micoplásmica; pneumonia enzoótica; diagnóstico
topic mycoplasmal pneumonia; enzootic pneumonia; diagnosis
pneumonia micoplásmica; pneumonia enzoótica; diagnóstico
description Direct and indirect immunofluorescence techniques and a culture method were compared for detection of Mycoplasma hyopneumoniae in lungs of experimentally infected swine. Pneumonic lungs were obtained from SPF pigs that had been exposed by contact 6 to 7 weeks previously to other SPF pigs which had been inoculated with the organism. A good association was found between isolation of M. hyopneumoniae, positive immunofluorescence test results and presence of macroscopic or microscopic lesions typical of mycoplasmal pneumonia of swine. Direct and indirect immunofluorescence tests were equally efficient for detection of M. hyopneumoniae in sections of lung. An azo dye counterstain reduced nonspecific fluorescence and provided a good color contrast without reducing sensitivity of the indirect immunofluorescence test.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-13
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15894
url https://seer.sct.embrapa.br/index.php/pab/article/view/15894
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/15894/9982
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.20, n.8, ago. 1985; 877-882
Pesquisa Agropecuária Brasileira; v.20, n.8, ago. 1985; 877-882
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416707570663424

Registros relacionados