Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee

Detalhes bibliográficos
Autor(a) principal: Pereira, Gabriella Santos
Data de Publicação: 2012
Outros Autores: Padilha, Lilian, Von Pinho, Edila Vilela Resende, Teixeira, Rita de Kássia Siqueira, de Carvalho, Carlos Henrique Siqueira, Maluf, Mirian Peres, de Carvalho, Bruna Line
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/10935
Resumo: The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty‑six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 rogenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST‑SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter‑ and intraspecific polymorphism in C. arabica and C. racemosa.
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spelling Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffeeMarcadores microssatélites na análise de resistência ao bicho‑mineiro em café arábicaCoffea arabica; Leucoptera coffeella; EST-SSR; genotypesCoffea arabica; Leucoptera coffeella; EST-SSR; genótiposThe objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty‑six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 rogenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST‑SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter‑ and intraspecific polymorphism in C. arabica and C. racemosa.O objetivo deste trabalho foi analisar genótipos de cafeeiro (Coffea arabica) resistentes ao bicho-mineiro (Leucoptera coffeella), com uso de marcadores microssatélites. Foram avaliados 66 locos, dos quais 63 foram obtidos do banco de sequências expressas (EST) de café. Esses locos foram amplificados em “bulks” de progênies F5 de 'Siriema' (C. arabica x C. racemosa) resistentes e suscetíveis ao ataque da praga, em oito acessos de C. racemosa, e em uma população F6 de 'Siriema' com 91 indivíduos que segregam para resistência ao bicho-mineiro. Os polimorfismos foram verificados em dois locos de marcadores microssatélites (SSR), nos “bulks” das progênies suscetíveis. Os dois alelos polimórficos estiveram presentes em torno de 70% dos genótipos suscetíveis em F5 e em, aproximadamente, 90% dos indivíduos suscetíveis em F6. Entretanto, os marcadores EST‑SSR polimórficos entre populações contrastantes para a resistência ao bicho-mineiro não foram correlacionados às características avaliadas. Os marcadores SSR apresentam polimorfismo inter e intraespecífico em C. arabica e C. racemosa.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraConsórcio Pesquisa Café (CBP&D Café), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig).Consórcio Pesquisa Café (CBP&D Café), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e a Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig).Pereira, Gabriella SantosPadilha, LilianVon Pinho, Edila Vilela ResendeTeixeira, Rita de Kássia Siqueirade Carvalho, Carlos Henrique SiqueiraMaluf, Mirian Peresde Carvalho, Bruna Line2012-02-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10935Pesquisa Agropecuaria Brasileira; v.46, n.12, dez. 2011; 1650-1656Pesquisa Agropecuária Brasileira; v.46, n.12, dez. 2011; 1650-16561678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/10935/6699info:eu-repo/semantics/openAccess2014-05-12T18:58:50Zoai:ojs.seer.sct.embrapa.br:article/10935Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-05-12T18:58:50Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
Marcadores microssatélites na análise de resistência ao bicho‑mineiro em café arábica
title Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
spellingShingle Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
Pereira, Gabriella Santos
Coffea arabica; Leucoptera coffeella; EST-SSR; genotypes
Coffea arabica; Leucoptera coffeella; EST-SSR; genótipos
title_short Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_full Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_fullStr Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_full_unstemmed Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
title_sort Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee
author Pereira, Gabriella Santos
author_facet Pereira, Gabriella Santos
Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
de Carvalho, Carlos Henrique Siqueira
Maluf, Mirian Peres
de Carvalho, Bruna Line
author_role author
author2 Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
de Carvalho, Carlos Henrique Siqueira
Maluf, Mirian Peres
de Carvalho, Bruna Line
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consórcio Pesquisa Café (CBP&D Café), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig).
Consórcio Pesquisa Café (CBP&D Café), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) e a Fundação de Amparo a Pesquisa do Estado de Minas Gerais (Fapemig).
dc.contributor.author.fl_str_mv Pereira, Gabriella Santos
Padilha, Lilian
Von Pinho, Edila Vilela Resende
Teixeira, Rita de Kássia Siqueira
de Carvalho, Carlos Henrique Siqueira
Maluf, Mirian Peres
de Carvalho, Bruna Line
dc.subject.por.fl_str_mv Coffea arabica; Leucoptera coffeella; EST-SSR; genotypes
Coffea arabica; Leucoptera coffeella; EST-SSR; genótipos
topic Coffea arabica; Leucoptera coffeella; EST-SSR; genotypes
Coffea arabica; Leucoptera coffeella; EST-SSR; genótipos
description The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty‑six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 rogenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST‑SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter‑ and intraspecific polymorphism in C. arabica and C. racemosa.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-10
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10935
url https://seer.sct.embrapa.br/index.php/pab/article/view/10935
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10935/6699
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.12, dez. 2011; 1650-1656
Pesquisa Agropecuária Brasileira; v.46, n.12, dez. 2011; 1650-1656
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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